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001-es BibID:	BIBFORM074287
Első szerző:	Soltész Beáta (molekuláris biológus)
Cím:	Cell-free miRNAs as potential biomarkers in the diagnosis of ovarian cancer / Beáta Soltész, Orsolya Biró, János Lukács, András Penyige, Róbert Póka, Bálint Nagy
Dátum:	2018
Megjegyzések:	cause of cancer mortality among women due to the poor early diagnostic tools for the detection of this type of aggressive tumour. Cell-free microRNAs appear to be potential candidate biomarker in this early diagnosis.Materials and methods: Eleven ovarian cancer patients and 11 healthy controls were involved into the study. EDTA-anticoagulated blood was drawn; the microRNA was isolated with miRNeasy Mini kit (Qiagen, Germany) and cDNA was synthesised. The miR-103; miR-125, miR-193b and miR-200b expression were determined by miRCURY Universal RT microRNA PCR Starter kit (Exiqon, USA). The results were normalised to the miRNA-93 reference gene. Student t-test was used for the statistical calculations.Results: Significant difference in the expression of miR-103 (p=0.001), miR-193b (p=0.0236), miR-125 (p=0.029) and miR-200b (p=0.0273) was detected among the cancer patients and controls.Conclusion: The expression of four different cell-free microRNAs was determined from the plasma of patients with ovarian cancer and healthy controls. Significant difference was found in the expression of the analysed microRNAs. In the near future, our study will be extended on higher number of cases.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt ovarian cancer mirNA cell-free
Megjelenés:	Biomedical Papers. - 162 : Suppl. 1 (2018), p. S13. -
További szerzők:	Biró Orsolya (molekuláris biológus) Lukács János (1975-) (szülész-nőgyógyász, genetikus) Penyige András (1954-) (molekuláris genetikus) Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus) Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM074285
Első szerző:	Szilágyi Melinda (biológus)
Cím:	MiR200a, miR200b and miR200c are promising candidate biomarkers in epithelial ovarian cancer / Melinda Szilágyi-Bónizs, Éva Márton, János Lukács, Beáta Soltész, Eszter Janka, András Penyige, Róbert Póka, Bálint Nagy
Dátum:	2018
Megjegyzések:	Ovarian cancer is the fifth most common form of cancer death among women. Developing a fast, reliable blood test would facilitate the early detection of ovarian cancer, which would also contribute to the improvement of survival chances. Screening circulating miRNAs proved to be reliable biomarkers in various cancers. MiRNAs are also dysregulated in ovarian cancer, however, only a few publications focus on their diagnostic role. We screened 6 miRNAs in the plasma samples of healthy (n=30), previously untreated epithelial ovarian carcinoma patients (n=21, FIGO stages III or IV) and patients with benign masses (n=14). The relative amount of miRNAs was detected by qRT-PCR.The expression levels of miR200a, miR200b and miR200c were elevated in the malignant samples compared to the healthy donors (p<0.001). The level of miR200a was also higher in malignant than benign samples (p<0.01). However, no significant difference was detected in the expression of miR205, miR483 and let7f. ROC-AUC was the highest in the case of miR200a (0.875, 95%CI=0.76-0.99) and it was still promising in the case of miR200b (0.85, 95%CI=0.72-0.98) and miR200c (0.83, 95%CI=0.70-0.97). Spearman's rank correlation was relatively high between the expression values of miR200b and miR200c (rs = 0.858; p<0.001) and these miRNAs shared several target genes involved in cancer development according to target prediction analysis. The agreement of diagnostic tests based on miR200s and CA125 or HE4 proved to be weak according to Cohen's kappa values.We conclude that miR200a, miR200b and miR200c might be promising complementary markers of CA125 and HE4 in ovarian cancer. Moreover miR200a is also a reliable biomarker in the differential diagnosis of benign tumors.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt ovarian cancer cell-free mtDNA miRNA
Megjelenés:	Biomedical Papers. - 162 : Suppl. 1 (2018), p. S11. -
További szerzők:	Márton Éva (1992-) (biológus) Lukács János (1975-) (szülész-nőgyógyász, genetikus) Soltész Beáta (1987-) (molekuláris biológus) Janka Eszter Anna (1989-) (bőrgyógyász, népegészségügyi szakember) Penyige András (1954-) (molekuláris genetikus) Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus) Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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