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001-es BibID:BIBFORM046867
Első szerző:Itoh, Masayoshi
Cím:Proteins that interact with GTP in Streptomyces griseus and its possible implication in morphogenesis / Itoh Masayoshi, Penyige Andras, Okamoto Susumu, Ochi Kozo
Dátum:1996
ISSN:0378-1097
Megjegyzések:By cross-linking with [?-32P]GTP or [?-32P]GTP with or without UV treatment, several proteins of Streptomyces griseus were shown to interact with GTP in specific ways. After gel electrophoresis, 19 bands of radioactivity were found; 12 bands were assigned as GTP-binding proteins and 6 bands as phosphorylated proteins. One band was assumed to be a guanylylated protein. The profile of radioactive bands was similar between cells prepared from liquid or solid culture, but markedly different between growth phases. A mutant (strain M-1) defective in aerial mycelium formation, which was originally found as a decoyinine-resistant isolate, was found to have a different profile of phosphorylated proteins.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Fems Microbiology Letters. - 135 : 2-3 (1996), p. 311-316. -
További szerzők:Penyige András (1954-) (molekuláris genetikus) Okamoto, Susumu Ochi, Kozo
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2.

001-es BibID:BIBFORM045833
035-os BibID:PMID:2120108
Első szerző:Penyige András (molekuláris genetikus)
Cím:ADP-ribosylation of membrane proteins of Streptomyces griseus strain 52-1 / Penyige, A., Barabas, Gy., Szabo, I., Ensign, J. C.
Dátum:1990
ISSN:0378-1097
Megjegyzések:Membranes purified from cells of Streptomyces griseus strain 52-1 possess and ADP-ribosyltransferase acrivity. The enzyme transfers the DP-ribose moiety of NAD to one major membrane protein of Mr 32000 and 2?3 minot proteins of larger molecular weights. The effects of inhibitors on the ADP-ribosyltransferase activity proves that the reaction is enzymatic and suggests that the enzyme ADP-ribosylates the guanidine group of arginine. The kinetics of liberation of ADP-ribose during alkaline hydrolysis of the modified proteins is consistent with the arginine-ADP-ribose bond. This is the first report of ADP-ribosylation of proteins in a Gram-positive bacterium.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
ADP-ribosylation
NAD-glycohydrolase
Protein modification
Streptomyces griseus
Megjelenés:Fems Microbiology Letters. - 57 : 3 (1990), p. 293-297. -
További szerzők:Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Szabó I. Ensign, J. C.
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3.

001-es BibID:BIBFORM004636
035-os BibID:(scopus)0031763323 (wos)000077220800013
Első szerző:Radwan, S. S.
Cím:Hydrocarbon uptake by streptomyces / Radwan, S. S., Barabas, G., Sorkhoh, N. A., Damjanovich, S., Szabo, I., Szollosi, J., Matko, J., Penyige, A., Hirano, T., Szabo, I. M.
Dátum:1998
ISSN:378-1097
Megjegyzések:Oligocarbophilic Streptomyces strains capable of hydrocarbon uptake and utilization were isolated from the polluted desert of Kuwait and used in this study. Transmission electron-microscopy of hyphae revealed that they become enriched with large less electron dense areas in the cytoplasm, when biomass samples were incubated with alkanes. The Streptomyces isolate could utilize n-hexadecane as sole carbon and energy source and their fatty acid content showed an increase in the fatty acids with chain length equivalent to those of the alkane substrates. Fluorescence measurements of diphenylhexatriene dissolved in the representative alkane, n-hexadecane, showed that the kinetics of hydrocarbon uptake are quite different in hydrocarbon-utilizer compared with non-utilizer Streptomyces strain. Microviscosity of the cellular membrane of the utilizer strain was also different from that of the non-utilizer control strain Streptomyces griseus after incubation in the presence of n-hexadecane. Very likely the hydrocarbon utilizer transported these compounds more efficiently across their membranes and accumulated them as inclusions in the cytoplasm
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Alkanes
Biomass
Cell Membrane
Cytoplasm
Desert Climate
Diphenylhexatriene
Environmental Microbiology
Environmental Pollution
Extraction and Processing Industry
Fatty Acids
Fluorescence
isolation and purification
Kinetics
Kuwait
metabolism
Petroleum
Streptomyces
Support
ultrastructure
Non-U.S.Gov't
Megjelenés:FEMS Microbiology Letters. - 169 : 1 (1998), p. 87-94. -
További szerzők:Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Sorkhoh, N. A. Damjanovich Sándor (1936-2017) (biofizikus) Szabó I. Szöllősi János (1953-) (biofizikus) Matkó János (1952-) (biológus) Penyige András (1954-) (molekuláris genetikus) Hirano, Tadashi Szabó I. M.
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