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001-es BibID:	BIBFORM009630
Első szerző:	Hádáné Birkó Zsuzsanna (molekuláris genetikus)
Cím:	Lack of A-factor production induces the expression of nutrient scavenging and stress-related proteins in Streptomyces griseus / Birkó Zsuzsanna, Swiatek Magdalena, Szájli Emília, Medzihradszky F. Katalin, Vijgenboom Erik, Penyige András, Keserű Judit, van Wezel Gilles P., Biró Sándor
Dátum:	2009
ISSN:	1535-9476 (Print)
Megjegyzések:	The small gamma-butyrolactone A-factor is an important autoregulatory signaling molecule for the soil-inhabiting streptomycetes. Starvation is a major trigger for development,and nutrients are provided by degradation of the vegetative mycelium via a process of programmed cell death, reusing proteins, nucleic acids, and cell wall material. The A-factor regulon includes many extracellular hydrolases. Here we show via proteomics analysis that many nutrientscavenging and stress-related proteins were overexpressed in an A-factor non-producing mutant of Streptomyces griseus B-2682. Transcript analysis showed that this is primarily due to differential transcription of the target genes during early development. The targets include proteins relating to nutrient stress and environmental stress and an orthologue of the Bacillus sporulation control protein Spo0M. The enhanced expression of these proteins underlines the stress that is generated by the absence of A-factor. Wild-type developmental gene expression was restored to the A-factor non-producing mutant by the signaling protein Factor C in line with our earlier observation that Factor C triggers A-factor production.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban C-faktor Streptomyces griseus proteomika
Megjelenés:	Molecular and Cellular Proteomics : MCP - 8 : 10 (2009), p. 2396-2403. -
További szerzők:	Swiatek, Magdalena Szájli Emília Medzihradszky-Fölkl Katalin Vijgenboom, Erik Penyige András (1954-) (molekuláris genetikus) Keserű Judit (1976-) (molekuláris genetikus) Wezel, Gilles P., van Biró Sándor (1949-) (molekuláris genetikus)
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001-es BibID:	BIBFORM001540
Első szerző:	Hádáné Birkó Zsuzsanna (molekuláris genetikus)
Cím:	The secreted signaling protein factor C triggers the A-factor response regulon in streptomyces griseus : overlapping signaling routes / Birkó Z., Bialek S., Buzás K., Szájli E., Traag B. A., Medzihradszky K. F., Rigali S., Vijgenboom E., Penyige A., Kele Z., van Wezel G. P., Bíró S.
Dátum:	2007
Megjegyzések:	Members of the prokaryotic genus Streptomyces produce over 60% of all known antibiotics and a wide range of industrial enzymes. A leading theme in microbiology is which signals are received and transmitted by these organisms to trigger the onset of morphological differentiation and antibiotic production. The small -butyrolactone A-factor is an important autoregulatory signaling molecule in streptomycetes, and A-factor mutants are blocked in development and antibiotic production. In this study we showed that heterologous expression of the 324-amino acid secreted regulatory protein Factor C resulted in restoration of development and enhanced antibiotic production of an A-factor-deficient bald mutant of Streptomyces griseus, although the parental strain lacks an facC gene. Proteome analysis showed that in the facC transformant the production of several secreted proteins that belong to the A-factor regulon was restored. HPLC-MS/MS analysis indicated that this was due to restoration of A-factor production to wild-type levels in the transformant. This indicates a connection between two highly divergent types of signaling molecules and possible interplay between their regulatory networks.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Streptomyces szignalizáció
Megjelenés:	Molecular and Cellular Proteomics : MCP - 6 : 7 (2007), p. 1248-1256. -
További szerzők:	Bialek, Sylwia Buzás Krisztina Szájli Emília Traag, Bjorn A. Medzihradszky-Fölkl Katalin Rigali, Sebastien Vijgenboom, Erik Kele Zoltán Wezel, Gilles P., van Biró Sándor (1949-) (molekuláris genetikus) Penyige András (1954-) (molekuláris genetikus)
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001-es BibID:	BIBFORM039004
035-os BibID:	PMID:19851727
Első szerző:	Penyige András (molekuláris genetikus)
Cím:	Analysis and identification of ADP-ribosylated proteins of Streptomyces coelicolor M145 / András Penyige, Judit Keseru, Ferenc Fazakas, Iván Schmelczer, Krisztina Szirák, György Barabás, Sándor Biró
Dátum:	2009
ISSN:	1225-8873
Megjegyzések:	Mono-ADP-ribosylation is the enzymatic transfer of ADP-ribose from NAD(+) to acceptor proteins catalyzed by ADP-ribosyltransferases. Using m-aminophenylboronate affinity chromatography, 2D-gel electrophoresis, in-gel digestion and MALDI-TOF analysis we have identified eight in vitro ADP-ribosylated proteins in Streptomyces coelicolor, which can be classified into three categories: (i) secreted proteins; (ii) metabolic enzymes using NAD(+)/NADH or NADP(+)/NADPH as coenzymes; and (iii) other proteins. The secreted proteins could be classified into two functional categories: SCO2008 and SC05477 encode members of the family of periplasmic extracellular solute-binding proteins, and SCO6108 and SC01968 are secreted hydrolases. Dehydrogenases are encoded by SC04824 and SC04771. The other targets are GlnA (glutamine synthetase I., SC02198) and SpaA (starvation-sensing protein encoded by SC07629). SCO2008 protein and GlnA had been identified as ADP-ribosylated proteins in previous studies. With these results we provided experimental support for a previous suggestion that ADP-ribosylation may regulate membrane transport and localization of periplasmic proteins. Since ADP-ribosylation results in inactivation of the target protein, ADP-ribosylation of dehydrogenases might modulate crucial primary metabolic pathways in Streptomyces. Several of the proteins identified here could provide a strong connection between protein ADP-ribosylation and the regulation of morphological differentiation in S. coelicolor.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban protein ADP-ribosylation Streptomyces coelicolor MALDI-TOF 2-D PAGE egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal Of Microbiology. - 47 : 5 (2009), p. 549-556. -
További szerzők:	Fazakas Ferenc (1969-) (molekuláris biológus) Schmelczer Iván Keserű Judit (1976-) (molekuláris genetikus) Szirák Krisztina (1973-) (molekuláris genetikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Biró Sándor (1949-) (molekuláris genetikus)
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001-es BibID:	BIBFORM039003
035-os BibID:	PMID:22752904
Első szerző:	Szirák Krisztina (molekuláris genetikus)
Cím:	Disruption of SCO5461 gene coding for a mono-ADP-ribosyltransferase enzyme produces a conditional pleiotropic phenotype affecting morphological differentiation and antibiotic production in Streptomyces coelicolor / Krisztina Szirák, Judit Keserű, Sándor Biró, Iván Schmelczer, György Barabás, András Penyige
Dátum:	2012
ISSN:	1225-8873
Megjegyzések:	The SCO5461 gene of Streptomyces coelicolor A3(2) codes for an ADP-ribosyltransferase enzyme that is predicted to be a transmembrane protein with an extracellular catalytic domain. PCR-targeted disruption of the gene resulted in a mutant that differentiated normally on complex SFM medium; however, morphological differentiation in minimal medium was significantly delayed and this phenotype was even more pronounced on osmotically enhanced minimal medium. The mutant did not sporulate when it was grown on R5 medium, however the normal morphological differentiation was restored when the strain was cultivated beside the wild-type S. coelicolor M145 strain. Comparison of the pattern of ADP-ribosylated proteins showed a difference between the mutant and the wild type, fewer modified proteins were present in the cellular crude extract of the mutant strain. These results support our previous suggestions that protein ADP-ribosylation is involved in the regulation of differentiation and antibiotic production and secretion in Streptomyces.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Streptomyces egyetemen (Magyarországon) készült közlemény mono-ADP-ribosyltransferase protein ADP-ribosylation morphological differentiation actinorhodin production and secretion
Megjelenés:	Journal of Microbiology. - 50 : 3 (2012), p. 409-418. -
További szerzők:	Schmelczer Iván Keserű Judit (1976-) (molekuláris genetikus) Biró Sándor (1949-) (molekuláris genetikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Penyige András (1954-) (molekuláris genetikus)
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