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001-es BibID:	BIBFORM004678
035-os BibID:	(scopus)0035153667 (wos)000171533400007
Első szerző:	Barabás György (sejtbiológus, molekuláris genetikus)
Cím:	N-Alkane uptake and utilisation by Streptomyces strains / Barabas, G., Vargha, G., Szabo, I. M., Penyige, A., Damjanovich, S., Szollosi, J., Matko, J., Hirano, T., Matyus, A., Szabo, I.
Dátum:	2001
Megjegyzések:	Streptomyces strains isolated from the Kuwait Burgan oil field were defined as S. griseoflavus, S. parvus, and S. plicatus utilised n-hexadecane, n-octadecane (purified fractions of mineral oil), kerosene, and crude oil as sole carbon and energy sources. The strains were incubated with n-alkanes and increase of the fatty acid content with chain length equivalent to the employed n-alkanes was observed. Signal transducing GTP-binding proteins (GBPs) play an important role in n-alkane uptake in streptomycetes. Specific activators of GBPs increased the uptake of hydrocarbons. Using the hydrophobic fluorescent dye diphenylhexatrien (DPH) as a probe, it was found that the microviscosity of the hydrophobic inner region of the cellular membrane is significantly lower in hydrocarbon utilisers than in non-utilisers. This difference probably reflects differences in the fatty acid composition of the strains. When cultures were grown in n-alkane containing media, electron microscopy revealed that the hydrocarbon utilisers showed less-electron dense areas as inclusions in the cytoplasm. Soil samples inoculated with Streptomyces strains eliminated hydrocarbons much faster than those not containing these strains, serving as control. When inorganic medium was supplied with n-hexadecane-1-14C as sole carbon and energy source, radioactive CO2 was detected. Since streptomycetes have not been used until now for oil elimination, though they are known as abundant soil bacteria tolerating extreme conditions, their possible use for bioremediation of hydrocarbon contaminated soils is discussed.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Alkanes analogs and derivatives analysis Biodegradation Cell Membrane Cell Membrane Permeability chemistry Cytoplasm Diphenylhexatriene Dyes Fatty Acids Fluorescent Dyes genetics GTP-Binding Proteins Human Hungary Hydrocarbons metabolism Microscopy physiology Streptomyces Support,Non-U.S.Gov't ultrastructure
Megjelenés:	Antonie Van Leeuwenhoek. - 79 : 3-4 (2001), p. 269-276. -
További szerzők:	Vargha György (1951-) (orvos) Szabó István M. Penyige András (1954-) (molekuláris genetikus) Damjanovich Sándor (1936-2017) (biofizikus) Szöllősi János (1953-) (biofizikus) Matkó János (1952-) (biológus) Hirano, Tadashi Mátyus Anita Szabó István
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001-es BibID:	BIBFORM045832
035-os BibID:	PMID:2127670
Első szerző:	Szabó István
Cím:	Effect of aminoglycoside antibiotics on the autolytic enzyme of Streptomyces griseus / Szabó István, Penyige András, Barabás György, Barabás Judit
Dátum:	1990
ISSN:	0302-8933
Megjegyzések:	The isolated cell wall of Streptomyces griseus 52-1 strain labelled with fluorescein isothiocyanate (FITC) and containing wall-bound autolytic enzyme was lysed as a function of different cations. The autolysis was accelerated by aminoglycoside antibiotics (streptomycin and the structurally closely related neomycin) which have a polycationic character. Since this strain is a streptomycin producer it is suggested that streptomycin may have a regulatory function on autolysis.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Streptomyces griseus autolytic enzyme streptomycin
Megjelenés:	Archives of Microbiology. - 155 : 1 (1990), p. 99-102. -
További szerzők:	Penyige András (1954-) (molekuláris genetikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Barabás Judit
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