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001-es BibID:	BIBFORM096437
Első szerző:	Nagy Zsófia (molekuláris biológus)
Cím:	Effect of E2 and long control region polymorphisms on disease severity in human papillomavirus type 11 mediated mucosal disease : protein modelling and functional analysis / Zsófia Nagy, Zoltán Pethő, Gábor Kardos, Tamás Major, Attila Szűcs, Krisztina Szarka
Dátum:	2021
ISSN:	1567-1348
Megjegyzések:	Interaction of the long control region (LCR) and the E2 protein of HPV11s was studied by in silico modelling and in vitro functional analysis. Genomes of HPV11s from fifteen (six known and nine novel) patients (two solitary papillomas, eleven respiratory papillomatoses of different severity, one condyloma acuminatum and one cervical atypia) were sequenced; E2 polymorphisms were analysed in silico by protein modelling. E2 and LCR variants were cloned into pcDNA3.1+ expression vector and into pALuc reporter vector, respectively, transfected to HEp2 cells alone or in different combinations and the luciferase activity was measured. In the E2, the ubiquitous polymorphism K308R caused stronger binding between the dimers but did not alter DNA binding; E2s with this polymorphism were significantly less efficient than the reference in promoting LCR activity. The unique polymorphism Q86K changed the negative surface charge of E2 (Q86) to positive (K86). The unique polymorphisms S245F and N247T in the hinge region disrupt a probable phosphorylation site in a RXXS motif targeted by protein kinase A and B, but do not affect directly the amino acids critical to nuclear transport. Both unique patterns partly restored the LCR activating potential disrupted by K308R. A unique E2/E4 ORF with a 58-bp deletion leading to a frameshift and an early stop codon resulted in a practically nonfunctional E2, and was associated with a papillomatosis with dysplasia. When testing existing LCR-E2 combinations, LCR with intrinsically lower enhancer capacity was only marginally activated by its E2 (R308 and the deletion mutant), and did not significantly exceed the activity of the reference LCR without E2. Combined with more potent LCRs associated with more severe disease, the activity was significantly higher, but still significantly lower than LCRs with reference E2. In summary, LCR-E2 interaction determined by their polymorphisms may explain, at least partly, differences in disease severity.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Low-risk human papillomavirus Recurrent respiratory papillomatosis Genome polymorphisms Long control region Transactivating potential
Megjelenés:	Infection Genetics and Evolution. - 93 (2021), p. 1-13. -
További szerzők:	Pethő Zoltán (1989-) (orvos) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Major Tamás (1973-) (fül-orr-gégész) Szűcs Attila (1970-) (fül-orr-gégész) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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001-es BibID:	BIBFORM040793
Első szerző:	Szládek Györgyi
Cím:	High co-prevalence of genogroup 1 TT virus and human papillomavirus is associated with poor clinical outcome of laryngeal carcinoma / Szladek G., Juhasz A., Kardos G., Szoke K., Major T., Sziklai I., Tar I., Marton I., Konya J., Gergely L., Szarka K.
Dátum:	2005
ISSN:	0021-9746
Megjegyzések:	BACKGROUND: The aetiology and factors leading to the progression of laryngeal cancer are still unclear. Although human papillomavirus (HPV) has been suggested to play a role, reports concerning the effect of HPV infection on tumour development are controversial. Recently, transfusion transmitted virus (TTV) was suggested to play a role in certain infections as a causative or coinfecting agent. AIMS: To investigate whether the development and progression of laryngeal squamous cell carcinoma is associated with coinfection with TTV and HPV. METHODS: The prevalence of TTV and HPV was investigated using the polymerase chain reaction in tissue samples from 40 healthy individuals, 10 patients with recurrent papillomatosis, five patients with papillomatosis with malignant transformation, and 25 patients with laryngeal carcinoma. The obtained prevalence data were compared and analysed statistically. RESULTS: In the 11 patients with carcinoma who had metastasis or relapse there was a high rate of coinfection with genogroup 1 TTV and HPV (eight of 11), whereas in the 14 without tumour progression no coinfection was found. Coinfection was associated with significantly lower tumour free survival in patients with carcinoma (p < 0.001). Furthermore, four of five patients who had papillomatosis with malignant transformation were coinfected with genogroup 1 TTV and HPV. CONCLUSIONS: Although the nature of cooperation between HPV and TTV needs to be investigated further, coinfection with genogroup 1 TTV and HPV appears to be associated with poor clinical outcome in laryngeal cancer.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal Of Clinical Pathology. - 58 : 4 (2005), p. 402-405. -
További szerzők:	Juhász Attila (1970-) (szakorvos, klinikai mikrobiológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Szőke Krisztina Major Tamás (1973-) (fül-orr-gégész) Sziklai István (1954-) (fül-orr-gégész) Tar Ildikó (1967-) (fogszakorvos) Márton Ildikó (1954-) (fogszakorvos) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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