Találati lista | Tudóstér

001-es BibID:	BIBFORM001077
Első szerző:	Peles Ferenc (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
Cím:	Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary / F. Peles, M. Wagner, L. Varga, I. Hein, P. Rieck, K. Gutser, P. Keresztúri, G. Kardos, I. Turcsányi, B. Béri, A. Szabó
Dátum:	2007
Megjegyzések:	Staphylococcus aureus is a major foodborne pathogen due to its capability to produce a wide range of heat-stable enterotoxins. The primary purpose of this research was to characterize S. aureus isolates recovered from mammary quarter milk of mastitic cows and from bulk tank milk produced on Hungarian dairy farms of different sizes. Macrorestriction analysis of chromosomal DNA from S. aureus isolates was performed using the restriction enzyme SmaI followed by pulsed-field gel electrophoresis (PFGE). The prevalence rates of nine S. aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and of the toxic shock syndrome toxin 1 gene (tst) were determined by multiplex polymerase chain reaction (PCR). The bulk tank milks of 14 out of 20 farms were contaminated with S. aureus at levels of up to 6.0x103 CFU/ml. Farm size had no significant effect (PN0.05) on the S. aureus counts in bulk milk. The prevalence rates of penicillin resistance were 88.9% and 20.0% among the S. aureus recovered from mastitic quarter milk and bulk tank milk, respectively. After phenotypic characterization, a total of 59 S. aureus isolates were selected for genotyping. PFGE analysis revealed 22 distinct pulsotypes, including 14 main types and 8 subtypes, at a similarity level of 86%. Only one or two main types were observed on each of the farms tested, indicating a lack of genetic diversity among S. aureus isolates within farms, and there were only two pulsotypes which occurred on more than one farm. The PFGE patterns showed genetic relatedness between the S. aureus strains recovered from quarter milk and bulk milk on two large farms, implying that on farms having a high number of mastitic cows, S. aureus from infected udders may contaminate bulk milk and, subsequently, raw milk products. Sixteen (27.1%) of the S. aureus isolates tested by multiplex PCR were found to be positive for enterotoxin genes, with 15 of them carrying just one gene and one strain carrying two genes (seg and sei). The most commonly detected toxin genes were seb, sea, and sec, whereas none of our isolates possessed the see, seh, sej, or tst genes. On 75% of the dairy farms surveyed, no enterotoxigenic staphylococci were recovered from either mastitic quarter milk or bulk tank milk.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban milk Enterotoxin gene Staphylococcus aureus PFGE PCR Pulsotype
Megjelenés:	International Journal of Food Microbiology. - 118 : 2 (2007), p. 186-193. -
További szerzők:	Wagner, Martin Varga László Hein, Ingeborg Rieck, Petra Gutser, Klaus Keresztúri Péter (1975-) (biológus-ökológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Turcsányi Ibolya Béri Béla (1951-) (agrármérnök) Szabó András (1947-) (biológus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL DOI
Borító:
Saját polcon:

001-es BibID:	BIBFORM090744
035-os BibID:	(cikkazonosító)104
Első szerző:	Petróczki Flóra Mária (élelmiszer-biztonsági és -minőségi mérnök)
Cím:	Occurrence and Characteristics of Staphylococcus aureus in a Hungarian Dairy Farm during a Control Program / Flóra M. Petróczki, Ákos Pásztor, Kata D. Szűcs, Károly Pál, Gábor Kardos, Ervin Albert, Brigitta Horváth, Erika Ungvári, Béla Béri, Ferenc Peles
Dátum:	2021
ISSN:	2076-0817
Megjegyzések:	In this research, our aim was to assess the occurrence of Staphylococcus aureus in a Hungarian large-scale dairy farm during the S. aureus control program conducted in the course of our studies. Furthermore, the phenotypic and genotypic properties of the isolates (type of haemolysis, antibiotic susceptibility, staphylococcal enterotoxin (SE) gene carrying ability and spa type) were determined. S. aureus was detected in all bulk tank milk samples collected during this study. Two different spa types were identified among the 17 strains isolated in the farm. A total of 14 of the 17 studied strains (82%) showed ?-haemolysis on blood agar, 2/17 strains (12%) expressed double zone and 1/17 strains (6%) showed weak ?-haemolysis. All strains were susceptible to most antibiotics tested (cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, tetracycline and trimethoprim/sulphamethoxazole), but all strains were resistant to penicillin G. A total of 11 of the 17 strains (65%) were found to harbour seg, sei, selm, seln, selo genes; 4/17 strains (24%) harboured sei, selm, seln, selo genes and 2/17 strains (11%) harboured sei gene. Since the new SEs/SEls can also cause foodborne outbreaks potentially and all strains were found to be resistant to penicillin G, it is essential to decrease and keep the prevalence of S. aureus low in the dairy farm and the implementation of the S. aureus control program is also highly justified. The results showed that the S. aureus count decreased by the end of our studies, so the control program was proved to be effective.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk antibiotic resistance bulk milk enterotoxin microbiology spa typing Staphylococcus aureus
Megjelenés:	Pathogens. - 10 : 2 (2021), p. 1-12. -
További szerzők:	Pásztor Ákos Szűcs Kata Dorina (1992-) (biomérnök, biotechnológus) Pál Károly (1978-) (mikrobiológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Albert Ervin (1988-) (állatorvos) Horváth Brigitta (1992-) (okleveles táplálkozástudományi szakember, dietetikus) Ungvári Erika Béri Béla (1951-) (agrármérnök) Peles Ferenc (1979-) (mikrobiológia, élelmiszer-mikrobiológia, minőségügy)
Pályázati támogatás:	EFOP-3.6.3-VEKOP-16-2017-00008 EFOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: