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001-es BibID:	BIBFORM054788
Első szerző:	Damjanova, Ivelina
Cím:	From farm to fork follow-up of thermotolerant campylobacters throughout the broiler production chain and in human cases in a Hungarian county during a ten-months period / I. Damjanova, M. Jakab, T. Farkas, J. Mészáros, Zs. Galántai, I. Turcsányi, A. Bistyák, Á. Juhász, J. Pászti, I. Kiss, G. Kardos
Dátum:	2011
ISSN:	0168-1605
Tárgyszavak:	Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	International Journal Of Food Microbiology. - 150 : 2-3 (2011), p. 95-102. -
További szerzők:	Jakab M. Farkas T. Mészáros József Galántai Zsuzsanna Turcsányi I. Bistyák Andrea Juhász Á. Pászti Judit Kiss I. Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus)
Pályázati támogatás:	GVOP 3.1.1-2004-0472/3.0 GVOP
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001-es BibID:	BIBFORM004817
Első szerző:	Nógrády Noémi
Cím:	Prevalence and characterization of Salmonella infantis isolates originating from different points of the broiler chicken-human food chain in Hungary / Nógrády Noémi, Kardos Gábor, Bistyák Andrea, Turcsányi Ibolya, Mészáros Júlia, Galántai Zsuzsanna, Juhász Ágnes, Samu Péterné, Kaszanyitzky J. Éva, Pászti Judit, Kiss István
Dátum:	2008
Megjegyzések:	During the 10-month study period Salmonella contamination of broiler houses and the flocks reared in three farms (A, B and C), the slaughter houses where the flocks were slaughtered, as well as the carcass and retail raw meat products originating from them was investigated. In the broiler farm A five consecutive flocks, in the B and C farms one flock was sampled. Environmental samples were taken prior to the introductions. Environmental, drinking water, feed and faecal samples were collected regularly using standard methods. Before and during processing of the flocks, environmental and carcass samples were taken at the abattoirs. Salmonella contamination of the carcass, retail meat, as well as stool samples of farm and abattoir workers and from human illnesses registered in the same period and region were also examined. Isolation, sero-, phage- and antibiotic resistance typing, class 1 integron and plasmid profiling of the strains were performed; their genetic relationship was assessed by PFGE. Although the broiler house and the faecal samples of the 5 flocks of the farm A were negative for Salmonella, S. infantis was isolated from 20?100% of the abattoir carcass samples. The retail raw meat samples were 0?100% S. infantis positive. The environmental samples of farm B were Salmonella negative, but the examined flock was contaminated: S. infantis was identified from 43% of the faecal samples. This serotype was identified in 100% of the carcass and retail raw meat samples. From environmental samples taken before the arrival of the 1-day-old chicks in the broiler house C, S. infantis was cultured. S. infantis prevalence in the faecal samples was 35% and all the carcass and retail raw meat samples were S. infantis contaminated. Altogether 164 S. infantis strains were isolated out of which 145 were further characterized. The vast majority (142/145) of the strains belonged to phage types 217 and 213. All but one were characterized by the nalidixic acid-streptomycin-sulphonamide-tetracycline resistances, had an 885 bp class 1 integron and a large plasmid of > 168 kb in size. The strains showed ? 88.7% genetic similarity. The results obtained shows that the same multi-drug resistant S. infantis clone was spread from the examined broiler farms contaminating the slaughter and the retail meat and appeared in the human illnesses of the examined region that was earlier detected as the dominant clone characteristic of the broiler and human population of the whole country.
Tárgyszavak:	Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk PFGE broiler flocks
Megjelenés:	International Journal of Food Microbiology. - 127 : 1-2 (2008), p. 162-167. -
További szerzők:	Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Bistyák Andrea Turcsányi Ibolya Mészáros Júlia Galántai Zsuzsanna Juhász Ágnes Samu Péter Kaszanyitzky J. Éva Pászti Judit Kiss István (mikrobiológus)
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001-es BibID:	BIBFORM008947
Első szerző:	Tóth István (Budapest)
Cím:	Virulence genes and molecular typing of different groups of Escherichia coli O157 strains in cattle / Tóth István, Schmidt Herbert, Kardos Gábor, Lancz Zsuzsanna, Creuzburg Kristina, Damjanova Ivelina, Pászti Judit, Beutin Lothar, Nagy Béla
Dátum:	2009
Megjegyzések:	Characterization of an Escherichia coli O157 strain collection (n = 42) derived from healthy Hungarian cattle revealed the existence of diverse pathotypes. Enteropathogenic E. coli (EPEC; eae positive) appeared to be the most frequent pathotype (n = 22 strains), 11 O157 strains were typical enterohemorrhagic E. coli (EHEC; stx and eae positive), and 9 O157 strains were atypical, with none of the key stx and eae virulence genes detected. EHEC and EPEC O157 strains all carried eae-gamma, tir-gamma, tccP, and paa. Other virulence genes located on the pO157 virulence plasmid and different O islands (O island 43 [OI-43] and OI-122), as well as espJ and espM, also characterized the EPEC and EHEC O157 strains with similar frequencies. However, none of these virulence genes were detected by PCR in atypical O157 strains. Interestingly, five of nine atypical O157 strains produced cytolethal distending toxin V (CDT-V) and carried genes encoding long polar fimbriae. Macro-restriction fragment enzyme analysis (pulsed-field gel electrophoresis) revealed that these E. coli O157 strains belong to four main clusters. Multilocus sequence typing analysis revealed that five housekeeping genes were identical in EHEC and EPEC O157 strains but were different in the atypical O157 strains. These results suggest that the Hungarian bovine E. coli O157 strains represent at least two main clones: EHEC/EPEC O157:H7/NM (nonmotile) and atypical CDT-V-producing O157 strains with H antigens different from H7. The CDT-V-producing O157 strains represent a novel genogroup. The pathogenic potential of these strains remains to be elucidated.
Tárgyszavak:	Agrártudományok Állatorvosi tudományok idegen nyelvű folyóiratközlemény külföldi lapban E. coli O157
Megjelenés:	Applied and Environmental Microbiology. - 75 : 19 (2009), p. 6282-6291. -
További szerzők:	Schmidt, Herbert Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Lancz Zsuzsanna Creuzburg, Kristina Damjanova, Ivelina Pászti Judit Beutin, Lothar Nagy Béla (Budapest)
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