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1.

001-es BibID:BIBFORM091232
035-os BibID:(cikkazonosító)127 (Wos)000622069100001 (Scopus)85100582621
Első szerző:Balázs Bence (PhD hallgató)
Cím:The Role of Uniform Meropenem Usage in Acinetobacter baumannii Clone Replacement / Balázs Bence, Tóth Zoltán, Nagy Fruzsina, Kovács Renátó, Tóth Hajnalka, Nagy József Bálint, Tóth Ákos, Szarka Krisztina, Majoros László, Kardos Gábor
Dátum:2021
ISSN:2079-6382
Tárgyszavak:Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Antibiotics. - 10 : 2 (2021), p. 1-12. -
További szerzők:Tóth Zoltán (1990-) (molekuláris biológus) Nagy Fruzsina (1991-) (molekuláris biológus) Kovács Renátó László (1987-) (molekuláris biológus) Tóth Hajnalka (1993-) (általános orvos) Nagy József Bálint (1993-) (molekuláris biológus) Tóth Ákos Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus) Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus)
Pályázati támogatás:EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
Bolyai Research Scholarship of the Hungarian Academy of Sciences
Egyéb
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2.

001-es BibID:BIBFORM070600
Első szerző:Ebrahimi, Fatemeh
Cím:Comparison of rates of fecal colonization with extended-spectrum beta-lactamase-producing enterobacteria among patients in different wards, outpatients and medical students / Ebrahimi Fatemeh, Mózes Julianna, Monostori Júlia, Gorácz Orsolya, Fésűs Adina, Majoros László, Szarka Krisztina, Kardos Gábor
Dátum:2016
ISSN:0385-5600
Tárgyszavak:Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Microbiology And Immunology. - 60 : 5 (2016), p. 285-294. -
További szerzők:Mózes Julianna (1982-) (orvosdiagnosztikai laboratóriumi analitikus, okleveles népegészségügyi szakember) Monostori Júlia Kungler-Gorácz Orsolya (1985-) (gyógyszerész) Fésüs Adina (1978-) (gyógyszerész) Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus)
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3.

001-es BibID:BIBFORM062414
Első szerző:Ebrahimi, Fatemeh
Cím:Asymptomatic faecal carriage of ESBL producing Enterobacteriaceae in Hungarian healthy individuals and in long-term care applicants : a shift towards CTX-M producers in the community / Fatemeh Ebrahimi, Julianna Mózes, Júlia Mészáros, Ágnes Juhász, László Majoros, Krisztina Szarka, Gábor Kardos
Dátum:2016
Megjegyzések:Background: Faecal carriage of extended-spectrum beta- lactamase (ESBL) producing Enterobacteriaceae28 in healthy individuals was examined and compared to previous results obtained in such individuals a few29 years earlier.30 Methods: Faecal samples from 779 individuals screened for employment purposes and from 22531 applicants to long-term care (LTC) were screened between November 2013 and May 2014.32 Results: The overall rate of fecal carriage was 3.0% (30/1004). The carriage rate was significantly higher33 in applicants for LTC (5.3% vs. 2.3%; p=0.019). All isolates carried CTX-M ESBLs, with an34 overwhelming dominance of blaCTX-M-15 (84.4%) in both groups and in both E. coli and Klebsiella35 pneumoniae.36 Conclusions: The prevalences were comparable to those in the earlier study, but a marked decrease of the37 diversity of ESBL genes in E. coli from the employment screening group was found, suggesting that the38 ESBL-producing isolates originating from diverse sources are being replaced by highly successful blaCTX39M-15 producing strains.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
extended-spectrum beta-lactamases, Klebsiella pneumoniae, Escherichia coli
Megjelenés:Infectious Diseases 48 : 7 (2016), p. 557-559. -
További szerzők:Mózes Julianna (1982-) (orvosdiagnosztikai laboratóriumi analitikus, okleveles népegészségügyi szakember) Mészáros Júlia Juhász Ágnes Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus)
Pályázati támogatás:TÁMOP 4.2.4. A/2-11-1-2012-0001
TÁMOP
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4.

001-es BibID:BIBFORM031960
Első szerző:Fehér Enikő (molekuláris biológus, mikrobiológus)
Cím:Comparison of diversity of torque teno virus 1 in different mucosal tissues and disorders / Fehér Enikő, Kardos Gábor, Gáll Tamás, Kis Andrea, Gergely Lajos, Szarka Krisztina
Dátum:2011
ISSN:1217-8950
Megjegyzések:Diversity of TTV1 was assessed in the head and neck region in patients with potentially malignant (oral lichen planus, oral leukoplakia) and malignant lesions (oral and laryngeal squamous cell cancers) and was compared to that found in the uterine cervix (cervical atypia and cervical cancer) by directly sequencing the NG061-063 segment of ORF1. These sequences were classified by the formerly used genogroupgenotype system as well as by the newly accepted species classification by aligning with the corresponding region of the type sequences of the 29 TTV species. All sequences obtained during the study clustered together with the TTV1 type sequence; to express diversity within TTV1, genotypes and subtypes of the former classification were used.The commonest subtypes were 2c followed by 2b, 1a and 1b. Subtypes 2b and 2c were evenly distributed among cervical samples; subtype 1a was more frequent in patients with cervical atypia or cancer. Subtypes 2c was more frequent than 2b in head and neck lesions. In conclusion, genotype and even subtype distribution may be important in association with diseases, therefore using this classification for characterization of intraspecies diversity of TTV1 is proposed.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény hazai lapban
Megjelenés:Acta Microbiologica et Immunologica Hungarica 58 : 4 (2011), p. 319-337. -
További szerzők:Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Kis Andrea (1981-) (molekuláris biológus, mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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5.

001-es BibID:BIBFORM047703
035-os BibID:PMID:23649705
Első szerző:Gáll Tamás (molekuláris biológus, mikrobiológus)
Cím:Genomic differences in the background of different severity in juvenile-onset respiratory papillomatoses associated with human papillomavirus type 11 / Tamás Gáll, Andrea Kis, Tímea Zsófia Tatár, Gábor Kardos, Lajos Gergely, Krisztina Szarka
Dátum:2013
ISSN:0300-8584
Megjegyzések:This study aimed to compare complete genome sequences of human papillomavirus (HPV) type 11 from two solitary papillomas (considered minimally aggressive), two moderately (six and nine episodes) and two highly aggressive (30 and 33 episodes) juvenile-onset respiratory papillomatoses. Genomic regions were sequenced using the Sanger method; sequences were compared to available GenBank genomes. Activity of the long control region (LCR) was assessed in HEp-2 cell line using luciferase assays and compared to that of the reference (GenBank Accession Number M14119). Site-directed mutagenesis was performed to confirm the association of polymorphisms with differences in LCR activity. Eleven alterations resulted in amino acid changes in different open reading frames. A72E in E1 and Q86K in E2 proteins were exclusively present in a moderately aggressive disease, L1 alterations A476V and S486F were unique to a severe papillomatosis. HPV11s in both solitary papillomas had identical LCRs containing a T7546C polymorphism, which strongly attenuated LCR activity, as confirmed by site-directed mutagenesis. This strong attenuator polymorphism was also present in the other four genomes showing significantly higher activities, but in these other alterations with demonstrable but statistically not significant attenuating (A7413C, 7509 T deletion) or enhancing (C7479T, T7904A) effect on transactivating potential (as demonstrated by site-directed mutagenesis) were also detected. LCR activities corresponded well to severity, excepting the highly aggressive papillomatosis with the L1 alterations. Presence of intratypic variants cannot explain differences in severity of respiratory papillomatoses associated with HPV11; virulence seems to be determined by the interaction of multiple genetic differences.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Medical Microbiology and Immunology. - 202 : 5 (2013), p. 353-363. -
További szerzők:Kis Andrea (1981-) (molekuláris biológus, mikrobiológus) Tatár Tímea Zsófia Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
Pályázati támogatás:OTKA 73145
OTKA
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6.

001-es BibID:BIBFORM101129
035-os BibID:(cikkazonosító)340 (WoS)000762112800001 (Scopus)85125243628
Első szerző:Girardi Petra
Cím:Evaluation of the Safety, Tolerability and Immunogenicity of ShigETEC, an Oral Live Attenuated Shigella-ETEC Vaccine in Placebo-Controlled Randomized Phase 1 Trial / Petra Girardi, Shushan Harutyunyan, Irene Neuhauser, Katharina Glaninger, Orsolya Korda, Gábor Nagy, Eszter Nagy, Valéria Szijártó, Denes Pall, Krisztina Szarka, Gábor Kardos, Tamás Henics, Frank J. Malinoski
Dátum:2022
ISSN:2076-393X
Megjegyzések:Shigella spp. and enterotoxigenic Escherichia coli (ETEC) cause high morbidity and mortality worldwide, yet no licensed vaccines are available to prevent corresponding infections. A live attenuated non-invasive Shigella vaccine strain lacking LPS O-antigen and expressing the ETEC toxoids, named ShigETEC was characterized previously in non-clinical studies. Methods: ShigETEC was evaluated in a two-staged, randomized, double-blind and placebo-controlled Phase I clinical trial. A single dose of increasing amounts of the vaccine was given to determine the maximum tolerated dose and increasing number of immunizations were administered with an interval based on the duration of shedding observed. Results: Oral immunization with ShigETEC was well tolerated and safe up to 4-time dosing with 5 ? 1010 colony forming units. ShigETEC induced robust systemic immune responses against the Shigella vaccine strain, with IgA serum antibody dominance, as well as mucosal antibody responses evidenced by specific IgA in stool samples and in ALS (Antibodies in Lymphocyte Supernatant). Anti- ETEC toxin responses were detected primarily in the 4-times immunized cohort and for the heat-labile toxin correlated with neutralizing capacity. Conclusion: ShigETEC is a promising vaccine candidate that is scheduled for further testing in controlled human challenge studies for efficacy as well as in children in endemic setting for safety and immunogenicity.
Tárgyszavak:Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Shigella
enterotoxigenic E. coli
vaccine
oral
Phase 1 clinical study
ETEC toxins
Megjelenés:Vaccines. - 10 : 2 (2022), p. 1-15. -
További szerzők:Harutyunyan, Shushan Neuhauser, Irene Glaninger, Katharina Korda Orsolya Nagy Gábor Nagy Eszter (Arsanis Inc. Vienna, Austria) Szíjártó Valéria Páll Dénes (1967-) (belgyógyász, kardiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Henics Tamás Malinoski, Frank J.
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7.

001-es BibID:BIBFORM096437
Első szerző:Nagy Zsófia (molekuláris biológus)
Cím:Effect of E2 and long control region polymorphisms on disease severity in human papillomavirus type 11 mediated mucosal disease : protein modelling and functional analysis / Zsófia Nagy, Zoltán Pethő, Gábor Kardos, Tamás Major, Attila Szűcs, Krisztina Szarka
Dátum:2021
ISSN:1567-1348
Megjegyzések:Interaction of the long control region (LCR) and the E2 protein of HPV11s was studied by in silico modelling and in vitro functional analysis. Genomes of HPV11s from fifteen (six known and nine novel) patients (two solitary papillomas, eleven respiratory papillomatoses of different severity, one condyloma acuminatum and one cervical atypia) were sequenced; E2 polymorphisms were analysed in silico by protein modelling. E2 and LCR variants were cloned into pcDNA3.1+ expression vector and into pALuc reporter vector, respectively, transfected to HEp2 cells alone or in different combinations and the luciferase activity was measured. In the E2, the ubiquitous polymorphism K308R caused stronger binding between the dimers but did not alter DNA binding; E2s with this polymorphism were significantly less efficient than the reference in promoting LCR activity. The unique polymorphism Q86K changed the negative surface charge of E2 (Q86) to positive (K86). The unique polymorphisms S245F and N247T in the hinge region disrupt a probable phosphorylation site in a RXXS motif targeted by protein kinase A and B, but do not affect directly the amino acids critical to nuclear transport. Both unique patterns partly restored the LCR activating potential disrupted by K308R. A unique E2/E4 ORF with a 58-bp deletion leading to a frameshift and an early stop codon resulted in a practically nonfunctional E2, and was associated with a papillomatosis with dysplasia. When testing existing LCR-E2 combinations, LCR with intrinsically lower enhancer capacity was only marginally activated by its E2 (R308 and the deletion mutant), and did not significantly exceed the activity of the reference LCR without E2. Combined with more potent LCRs associated with more severe disease, the activity was significantly higher, but still significantly lower than LCRs with reference E2. In summary, LCR-E2 interaction determined by their polymorphisms may explain, at least partly, differences in disease severity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Low-risk human papillomavirus
Recurrent respiratory papillomatosis
Genome polymorphisms
Long control region
Transactivating potential
Megjelenés:Infection Genetics and Evolution. - 93 (2021), p. 1-13. -
További szerzők:Pethő Zoltán (1989-) (orvos) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Major Tamás (1973-) (fül-orr-gégész) Szűcs Attila (1970-) (fül-orr-gégész) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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8.

001-es BibID:BIBFORM082111
Első szerző:Szinai Mihály
Cím:Comparative analysis of human papillomavirus type 6 complete genomes originated from head and neck and anogenital disorders / Szinai Mihály, Nagy Zsófia, Máté Petra, Kovács Dávid, Laczkó Levente, Kardos Gábor, Sápy Tamás, Szűcs Attila, Szarka Krisztina
Dátum:2019
ISSN:1567-1348
Megjegyzések:It is increasingly recognized that fundamental differences exist between high-risk and low-risk human papillomavirus (HPV) genotypes regarding interactions with the host. This study aims to join the recently emerging efforts to uncover these differences at the complete genome level and to study how they may influence the disease caused. Sixteen samples of thirteen patients with various HPV6-mediated benign mucosal disorders (nine recurrent respiratory papillomatoses with 2-8 recurrences, one condyloma acuminatum and three premalignant lesions of the genital mucosa) were sampled to determine the complete virus genomes. We collected the 197 HPV6 complete genomes deposited in the GenBank for cluster analysis to determine (sub)lineages. Genome polymorphisms were determined against the reference sequences of the (sub)lineages. Genome polymorphisms of the long control region (LCR) were tested for putative transcription factor binding sites; their functional analysis was performed by transient transfection of cloned whole LCRs into HEp-2 cells using a luciferase reporter system. Genomes from the same patients were always identical. Three, nine and one patients carried HPV6 lineage A, sublineage B1 and B2 variants, respectively. The three lineage A sequences were highly similar to each other, but distinct from the reference genome. A unique non-synonymous single nucleotide polymorphism (SNP) was found in the E5a open reading frame (ORF). Sublineage B1 genomes were more diverse, exhibited unique non-synonymous SNPs in the LCR and the E2/E4, L1, L2 ORFs. LCR activity of lineage A and sublineage B1 differed significantly; activity of one sublineage B1 LCR exhibiting two unique SNPs was significantly higher than that of other B1 LCR variants, close to the mean of LCR activities of lineage A variants. Different HPV6 lineages showed marked differences in variability patterns of the different genome regions. This may be involved in the differences in their distribution in different diseases or patient populations.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Cervical atypia
Condyloma acuminatum
Genome polymorphisms
Intratypic variation
Low-risk human papillomavirus
Recurrent respiratory papillomatosis
Megjelenés:INFECTION GENETICS AND EVOLUTION. - 71 (2019), p. 140-150. -
További szerzők:Nagy Zsófia (1991-) (molekuláris biológus) Máté Petra Kovács Dávid (1989-) (fül-orr-gégész) Laczkó Levente (1992-) (biológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Sápy Tamás (1970-) (szülész-nőgyógyász) Szűcs Attila (1970-) (fül-orr-gégész) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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9.

001-es BibID:BIBFORM040793
Első szerző:Szládek Györgyi
Cím:High co-prevalence of genogroup 1 TT virus and human papillomavirus is associated with poor clinical outcome of laryngeal carcinoma / Szladek G., Juhasz A., Kardos G., Szoke K., Major T., Sziklai I., Tar I., Marton I., Konya J., Gergely L., Szarka K.
Dátum:2005
ISSN:0021-9746
Megjegyzések:BACKGROUND: The aetiology and factors leading to the progression of laryngeal cancer are still unclear. Although human papillomavirus (HPV) has been suggested to play a role, reports concerning the effect of HPV infection on tumour development are controversial. Recently, transfusion transmitted virus (TTV) was suggested to play a role in certain infections as a causative or coinfecting agent. AIMS: To investigate whether the development and progression of laryngeal squamous cell carcinoma is associated with coinfection with TTV and HPV. METHODS: The prevalence of TTV and HPV was investigated using the polymerase chain reaction in tissue samples from 40 healthy individuals, 10 patients with recurrent papillomatosis, five patients with papillomatosis with malignant transformation, and 25 patients with laryngeal carcinoma. The obtained prevalence data were compared and analysed statistically. RESULTS: In the 11 patients with carcinoma who had metastasis or relapse there was a high rate of coinfection with genogroup 1 TTV and HPV (eight of 11), whereas in the 14 without tumour progression no coinfection was found. Coinfection was associated with significantly lower tumour free survival in patients with carcinoma (p < 0.001). Furthermore, four of five patients who had papillomatosis with malignant transformation were coinfected with genogroup 1 TTV and HPV. CONCLUSIONS: Although the nature of cooperation between HPV and TTV needs to be investigated further, coinfection with genogroup 1 TTV and HPV appears to be associated with poor clinical outcome in laryngeal cancer.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Clinical Pathology. - 58 : 4 (2005), p. 402-405. -
További szerzők:Juhász Attila (1970-) (szakorvos, klinikai mikrobiológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Szőke Krisztina Major Tamás (1973-) (fül-orr-gégész) Sziklai István (1954-) (fül-orr-gégész) Tar Ildikó (1967-) (fogszakorvos) Márton Ildikó (1954-) (fogszakorvos) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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10.

001-es BibID:BIBFORM095312
Első szerző:Tóth Hajnalka (általános orvos)
Cím:Evolution of the Gram-Negative Antibiotic Resistance Spiral over Time : a Time-Series Analysis / Hajnalka Tóth, Gyula Buchholcz, Adina Fésüs, Bence Balázs, József Bálint Nagy, László Majoros, Krisztina Szarka, Gábor Kardos
Dátum:2021
ISSN:2079-6382
Megjegyzések:We followed up the interplay between antibiotic use and resistance over time in a tertiary-care hospital in Hungary. Dynamic relationships between monthly time-series of antibiotic consumption data (defined daily doses per 100 bed-days) and of incidence densities of Gram-negative bacteria (Escherichia coli, Klebsiella spp., Pseudomonas aeruginosa, and Acinetobacter baumannii) resistant to cephalosporins or carbapenems were followed using vector autoregressive models sequentially built of time-series ending in 2015, 2016, 2017, 2018, and 2019. Relationships with Gram-negative bacteria as a group were fairly stable across years. At species level, association of cephalosporin use and cephalosporin resistance of E. coli was shown in 2015?2017, leading to increased carbapenem use in these years. Association of carbapenem use and carbapenem resistance, as well as of carbapenem resistance and colistin use in case of A. baumannii, were consistent throughout; associations in case of Klebsiella spp. were rarely found; associations in case of P. aeruginosa varied highly across years. This highlights the importance of temporal variations in the interplay between changes in selection pressure and occurrence of competing resistant species.
Tárgyszavak:Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
vector autoregressive models
antibiotic consumption
antibiotic resistance
Escherichia coli
Klebsiella spp.
Pseudomonas aeruginosa
Acinetobacter baumannii
cephalosporin resistance
carbapenem resistance
colistin
Megjelenés:Antibiotics-Basel. - 10 : 6 (2021), p. 1-10. -
További szerzők:Buchholcz Gyula (1961-) (aneszteziológus) Fésüs Adina (1978-) (gyógyszerész) Balázs Bence (1991-) (PhD hallgató) Nagy József Bálint (1993-) (molekuláris biológus) Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus)
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11.

001-es BibID:BIBFORM076404
035-os BibID:(WoS)000490004300020 (Scopus)85070814450
Első szerző:Tóth Hajnalka (általános orvos)
Cím:Utilization of vector autoregressive and linear transfer models to follow up the antibiotic resistance spiral in Gram-negative bacteria from cephalosporin consumption to colistin resistance / Hajnalka Tóth, Adina Fésűs, Orsolya Kungler-Gorácz, Bence Balázs, László Majoros, Krisztina Szarka, Gábor Kardos
Dátum:2019
ISSN:1058-4838
Megjegyzések:BACKGROUND: Increasing antibiotic resistance may reciprocally affect consumption and lead to using broader-spectrum alternatives; a vicious cycle that may gradually limit therapeutic options. The study aims at demonstrating the abovementioned vicious cycle in Gram-negative bacteria and at showing the utility of vector autoregressive (VAR) models for analysis of time-series in explanatory and dependent roles simultaneously. METHODS: Monthly drug consumption data in defined daily doses per 100 bed-days and incidence densities of Gram-negative bacteria (Escherichia coli, Klebsiella spp., Pseudomonas aeruginosa and Acinetobacterbaumannii) resistant to cephalosporins or to carbapenems were analysed using VAR models. These were compared to linear transfer models used earlier. RESULTS: In case of all Gram-negative bacteria, cephalosporin consumption led to increasing cephalosporin resistance, which provoked carbapenem use and consequent carbapenem resistance, and finally increased colistin consumption, exemplifying the vicious cycle. Different species were involved in different manner, e.g. cephalosporin resistant Klebsiella spp. provoked carbapenem use less than E. coli or association between carbapenem resistance of P. aeruginosa and colistin use was weaker than in case of A. baumannii. Colistin use led to decreased carbapenem use and decreased carbapenem resistance of P. aeruginosa but not of A. baumannii. CONCLUSIONS: Vector autoregressive models allow analysis of consumption and resistance series in a bidirectional manner. The reconstructed resistance spiral involved cephalosporin use augmenting cephalosporin resistance primarily in E. coli. This led to increased carbapenem use, provoking spread of carbapenem resistant A. baumannii and consequent colistin use. Emergence of panresistance is fuelled by such antibiotic resistance spirals.
Tárgyszavak:Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
time-series analysis
antibiotic consumption
carbapenem resistance
Megjelenés:Clinical Infectious Diseases. - 69 : 8 (2019), p. 1410-1421. -
További szerzők:Fésüs Adina (1978-) (gyógyszerész) Kungler-Gorácz Orsolya (1985-) (gyógyszerész) Balázs Bence (1991-) (PhD hallgató) Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus)
Pályázati támogatás:ÚNKP-17-2
ÚNKP
Bolyai ösztöndíj
MTA
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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12.

001-es BibID:BIBFORM103796
035-os BibID:(cikkazonosító)e0274414 (WoS)000933375500038 (Scopus)85138452471
Első szerző:Váradi Alex (biológus)
Cím:Rapid genotyping of targeted viral samples using Illumina short-read sequencing data / Alex Váradi, Eszter Kaszab, Gábor Kardos, Eszter Prépost, Krisztina Szarka, Levente Laczkó
Dátum:2022
ISSN:1932-6203
Megjegyzések:The most important information about microorganisms might be their accurate genome sequence. Using current Next Generation Sequencing methods, sequencing data can be generated at an unprecedented pace. However, we still lack tools for the automated and accurate reference-based genotyping of viral sequencing reads. This paper presents our pipeline designed to reconstruct the dominant consensus genome of viral samples and analyze their within-host variability. We benchmarked our approach on numerous datasets and showed that the consensus genome of samples could be obtained reliably without further manual data curation. Our pipeline can be a valuable tool for fast identifying viral samples. The pipeline is publicly available on the project's GitHub page (https://github.com/laczkol/QVG).
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Plos One. - 17 : 9 (2022), p. 1-23. -
További szerzők:Kaszab Eszter (1989-) (biológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Prépost Eszter Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus) Laczkó Levente (1992-) (biológus)
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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