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001-es BibID:BIBFORM101910
035-os BibID:(cikkazonosító)397 (WoS)000787051700001 (Scopus)85129122957
Első szerző:Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:Unique and Repeated Stwintrons (Spliceosomal Twin Introns) in the Hypoxylaceae / Erzsébet Fekete, Fruzsina Pénzes, Norbert Ág, Viktória Ág-Rácz, Erzsébet Sándor, Claudio Scazzocchio, Michel Flipphi, Levente Karaffa
Dátum:2022
ISSN:2309-608X
Megjegyzések:Introns are usually non-coding sequences interrupting open reading frames in pre-mRNAs [D1,2]. Stwintrons are nested spliceosomal introns, where an internal intron splits a second donor sequence into two consecutive splicing reactions leading to mature mRNA. In Hypoxylon sp. CO27-5, 36 highly sequence-similar [D1,2] stwintrons are extant (sister stwintrons). An additional 81 [D1,2] sequence-unrelated stwintrons are described here. Most of them are located at conserved gene positions rooted deep in the Hypoxylaceae. Absence of exonic sequence bias at the exon?stwintron junctions and a very similar phase distribution were noted for both groups. The presence of an underlying sequence symmetry in all 117 stwintrons was striking. This symmetry, more pronounced near the termini of most of the full-length sister stwintrons, may lead to a secondary structure that brings into close proximity the most distal splice sites, the donor of the internal and the acceptor of the external intron. The Hypoxylon stwintrons were overwhelmingly excised by consecutive splicing reactions precisely removing the whole intervening sequence, whereas one excision involving the distal splice sites led to a frameshift. Alternative (mis)splicing took place for both sister and uniquely occurring stwintrons. The extraordinary symmetry of the sister stwintrons thus seems dispensable for the infrequent, direct utilisation of the distal splice sites.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
spliceosomal introns
splicing; stwintrons
comparative genomics
stwintron dissemination
stwintron propagation
internal symmetry
single-stranded RNA secondary structure prediction
Hypoxylon sp. CO27-5
Hypoxylaceae
Megjelenés:Journal of Fungi. - 8 : 4 (2022), p. 1-23. -
További szerzők:Pénzes Fruzsina (1995-) (okleveles biomérnök) Ág Norbert (1987-) (okleveles biomérnök) Ág-Rácz Viktória (1991-) (mikrobiológus) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Scazzocchio, Claudio Flipphi, Michel (1964-) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
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2.

001-es BibID:BIBFORM117383
035-os BibID:(cikkazonosító)1195
Első szerző:Flipphi, Michel
Cím:Generation, Transfer, and Loss of Alternative Oxidase Paralogues in the Aspergillaceae Family / Michel Flipphi, Alexandra Márton, Vivien Bíró, Norbert Ág, Erzsébet Sándor, Erzsébet Fekete, Levente Karaffa
Dátum:2023
ISSN:2309-608X
Megjegyzések:Abstract: Alternative oxidase (Aox) is a terminal oxidase operating in branched electron transport. The activity correlates positively with overflow metabolisms in certain Aspergilli, converting intracellular glucose by the shortest possible path into organic acids, like citrate or itaconate. Aox is nearly ubiquitous in fungi, but aox gene multiplicity is rare. Nevertheless, within the family of the Aspergillaceae and among its various species of industrial relevance?Aspergillus niger, A. oryzae, A. terreus, Penicillium rubens?paralogous aox genes coexist. Paralogous genes generally arise from duplication and are inherited vertically. Here, we provide evidence of four independent duplication events along the lineage that resulted in aox paralogues (aoxB) in contemporary Aspergillus and Penicillium taxa. In some species, three aox genes are co-expressed. The origin of the A. niger paralogue is different than that of the A. terreus paralogue, but all paralogous clades ultimately arise from ubiquitous aoxA parent genes. We found different patterns of uncorrelated gene losses reflected in the Aspergillus pedigree, albeit the original aoxA orthologues persist everywhere and are never replaced. The loss of acquired paralogues co-determines the contemporary aox gene content of individual species. In Aspergillus calidoustus, the two more ancient paralogues have, in effect, been replaced by two aoxB genes of distinct origins.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus
Penicillium
alternative oxidase
aox gene multiplicity
gene duplication
paralogous genes
gene loss
Megjelenés:Journal of Fungi. - 9 : 12 (2023), p. 1-18. -
További szerzők:Márton Alexandra (1994-) (mikrobiológus) Bíró Vivien (1994-)c(mikrobiológus) Ág Norbert (1987-) (okleveles biomérnök) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
Pályázati támogatás:ÚNKP-23-3-II-DE-359
Egyéb
ÚNKP-23-4-IDE-362
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM111705
035-os BibID:(cikkazonosító)570 (Scopus)85160535117 (WoS)000997803000001
Első szerző:Flipphi, Michel
Cím:Mutations in the Second Alternative Oxidase Gene: A New Approach to Group Aspergillus niger Strains / Michel Flipphi, Alexandra Márton, Vivien Bíró, Norbert Ág, Erzsébet Sándor, Erzsébet Fekete, Levente Karaffa
Dátum:2023
ISSN:2309-608X
Megjegyzések:Alternative oxidase is a terminal oxidase in the branched mitochondrial electron transport chain of most fungi including Aspergillus niger (subgenus Circumdati, section Nigri). A second, paralogous aox gene (aoxB) is extant in some A. niger isolates but also present in two divergent species of the subgenus Nidulantes A. calidoustus and A. implicatus?as well as in Penicillium swiecickii. Black aspergilli are cosmopolitan opportunistic fungi that can cause diverse mycoses and acute aspergillosis in immunocompromised individuals. Amongst the approximately 75 genome-sequenced A. niger strains, aoxB features considerable sequence variation. Five mutations were identified that rationally affect transcription or function or terminally modify the gene product. One mutant allele that occurs in CBS 513.88 and A. niger neotype strain CBS 554.65 involves a chromosomal deletion that removes exon 1 and intron 1 from aoxB. Another aoxB allele results from retrotransposon integration. Three other alleles result from point mutations: a missense mutation of the start codon, a frameshift, and a nonsense mutation. A. niger strain ATCC 1015 has a full-length aoxB gene. The A. niger sensu stricto complex can thus be subdivided into six taxa according to extant aoxB allele, which may facilitate rapid and accurate identification of individual species
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
ubiquinol:oxygen oxidoreductase encoding gene
Aspergillus niger sensu stricto complex
alleles
chromosomal deletion
retrotransposon insertion
point mutation
frameshift mutation
nonsense mutation
molecular taxonomy
Megjelenés:Journal of Fungi. - 9 : 5 (2023), p. 1-17. -
További szerzők:Márton Alexandra (1994-) (mikrobiológus) Bíró Vivien (1994-)c(mikrobiológus) Ág Norbert (1987-) (okleveles biomérnök) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
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Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM075416
Első szerző:Molnár Ákos Péter (okleveles biomérnök)
Cím:High oxygen tension increases itaconic acid accumulation, glucose consumption, and the expression and activity of alternative oxidase in Aspergillus terreus / Ákos P. Molnár, Zoltán Németh, István S. Kolláth, Erzsébet Fekete, Michel Flipphi, Norbert Ág, Áron Soós, Béla Kovács, Erzsébet Sándor, Christian P. Kubicek, Levente Karaffa
Dátum:2018
ISSN:0175-7598 1432-0614
Megjegyzések:Itaconic acid is a five-carbon dicarboxylic acid with an unsaturated alkene bond, frequently used as a building block for the industrial production of a variety of synthetic polymers. It is also one of the major products of fungal "overflow metabolism" which can be produced in submerged fermentations of the filamentous fungus Aspergillus terreus. At the present, molar yields of itaconate are lower than those obtained in citric acid production in Aspergillus niger. Here, we have studied the possibility that the yield may be limited by the oxygen supply during fermentation and hence tested the effect of the dissolved oxygen concentration on the itaconic acid formation rate and yield in lab-scale bioreactors. The data show that a dissolved oxygen concentration of 2% saturation was sufficient for maximal biomass formation. Raising it to 30% saturation had no effect on biomass formation or the growth rate, but the itaconate yield augmented substantially from 0.53 to 0.85 mol itaconate/mol glucose. Furthermore, the volumetric and specific rates of itaconic acid formation ameliorated by as much as 150% concurrent with faster glucose consumption, shortening the fermentation time by 48 h. Further increasing the dissolved oxygen concentration over 30% saturation had no effect. Moreover, we show that this increase in itaconic acid production coincides with an increase in alternative respiration, circumventing the formation of surplus ATP by the cytochrome electron transport chain, as well as with increased levels of alternative oxidase transcript. We conclude that high(er) itaconic acid accumulation requires a dissolved oxygen concentration that is much higher than that needed for maximal biomass formation, and postulate that the induction of alternative respiration allows the necessary NADH reoxidation ratio without surplus ATP production to increase the glucose consumption and the flux through overflow metabolism.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Aspergillus terreus
Itaconic acid
Alternative oxidase
Respiration
Submerged fermentation
Dissolved oxygen
Megjelenés:Applied Microbiology and Biotechnology. - 102 : 20 (2018), p. 8799-8808. -
További szerzők:Németh Zoltán (1987-) (biomérnök) Kolláth István Sándor (biomérnök) Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Flipphi, Michel (1964-) Ág Norbert (1987-) (okleveles biomérnök) Soós Áron (1990-) (élelmiszerbiztonsági és -minőségi mérnök) Kovács Béla (1963-) (okleveles vegyész, angol szakfordító) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Kubicek, Christian P. Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
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