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001-es BibID:	BIBFORM036993
Első szerző:	Borbásné Sebestyén Veronika (biofizikus)
Cím:	Membrane potential influences mobility, interactions and signaling of interleukin-2 and-15 receptors in T cells / Sebestyén Veronika, Nagy Éva, Mocsár Gábor, Papp Ferenc, Hajdú Péter, Panyi György, Tóth Katalin, Thomas A. Waldmann, Damjanovich Sándor, Bodnár Andrea, Vámosi György
Dátum:	2011
Tárgyszavak:	Természettudományok Biológiai tudományok idézhető absztrakt MHC I interleukin-2 receptor IL-15 receptor protein cluster FRET IL-2 signaling Molekuláris Medicina Molekuláris Medicina
Megjelenés:	European Biophysics Journal with Biophysics Letters. - 40 : 1 (2011), p. 86. -
További szerzők:	Nagy Éva (1980-) (molekuláris biológus) Mocsár Gábor (1981-) (biofizikus) Papp Ferenc (1979-) (biofizikus) Hajdu Péter (1975-) (biofizikus) Panyi György (1966-) (biofizikus) Tóth Katalin Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Vámosi György (1967-) (biofizikus)
Pályázati támogatás:	K77600 OTKA TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Receptor tirozin kinázok mint terápiás célpontok: működésük szabályozásának, és a közöttük fellépő molekuláris kölcsönhatások vizsgálata TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP K+ csatornák élettani szerepe az immunrendszer sejtjeiben
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM004884
Első szerző:	Rubovszky Bálint (élettanász)
Cím:	Detection of channel proximity by nanoparticle-assisted delaying of toxin binding : a combined patch-clamp and flow cytometric energy transfer study / Rubovszky, B., Hajdu, P., Krasznai, Z., Gaspar, R., Waldmann, T. A., Damjanovich, S., Bene, L.
Dátum:	2005
ISSN:	0175-7571
Megjegyzések:	Gold nanoparticles of 30 nm diameter bound to cell-surface receptor major histocompatibility complex glycoproteins (MHCI and MHCII), interleukin-2 receptor alpha subunit (IL-2Ralpha), very late antigen-4 (VLA-4) integrin, transferrin receptor, and the receptor-type protein tyrosin phosphatase CD45 are shown by the patch-clamp technique to selectively modulate binding characteristics of Pi(2) toxin, an efficient blocker of K(v)1.3 channels. After correlating the electrophysiological data with those on the underlying receptor clusters obtained by simultaneously conducted flow cytometric energy transfer measurements, the modulation was proved to be sensitive to the density and size of the receptor clusters, and to the locations of the receptors as well. Based on the observation that engagement of MHCII by a monoclonal antibody down-regulates channel current and based on the close nanometer-scale proximity of the MHCI and MHCII glycoproteins, an analogous experiment was carried out when gold nanoparticles bound to MHCI delayed down-regulation of the K(v)1.3 current initiated by ligation of MHCII. Localization of K(v)1.3 channels in the nanometer-scale vicinity of the MHC-containing lipid rafts is demonstrated for the first time. A method is proposed for detecting receptor-channel or receptor-receptor proximity by observing nanoparticle-induced increase in relaxation times following concentration jumps of ligands binding to channels or to receptors capable of regulating channel currents.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Antibodies Biophysics Cell Line chemistry drug effects Energy Transfer Flow Cytometry Glycoproteins Gold Human Humans Interleukin-2 Interleukin-2 Receptor alpha Subunit Ion Channel Gating Kv1.3 Potassium Channel Ligands Major Histocompatibility Complex methods Nanotubes Particle Size Patch-Clamp Techniques pharmacokinetics physiology Potassium Potassium Channels Potassium Channels,Voltage-Gated Protein Binding Research Scorpion Venoms Support T-Lymphocytes
Megjelenés:	European Biophysics Journal. - 34 : 2 (2005), p. 127-143. -
További szerzők:	Hajdu Péter (1975-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus) Bene László (1963-) (biofizikus)
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001-es BibID:	BIBFORM005515
Első szerző:	Somodi Sándor (belgyógyász)
Cím:	Effects of changes in extracellular pH and potassium concentration on Kv1.3 inactivation / Somodi S., Hajdu P., Gáspár R., Panyi G., Varga Z.
Dátum:	2008
Megjegyzések:	The Kv1.3 channel inactivates via the P/C-type mechanism, which is influenced by a histidine residue in the pore region (H399, equivalent of Shaker 449). Previously we showed that the electric field of the protonated histidines at low extracellular pH (pH(e)) creates a potential barrier for K(+) ions just outside the pore that hinders their exit from the binding site controlling inactivation (control site) thereby slowing inactivation kinetics. Here we examined the effects of extracellular potassium [K(+)](e) and pH(e) on the rate of inactivation of Kv1.3 using whole-cell patch-clamp. We found that in 150 mM [K(+)](e )inactivation was accelerated upon switching to pH(e) 5.5 as opposed to the slowing at 5 mM [K(+)](e). The transition from slowing to acceleration occurred at 40 mM [K(+)](e), whereas this "turning point" was at 20 mM [K(+)](e) for inward currents. The rate of entry of Ba(2+) ions from the extracellular space to the control site was significantly slowed by low pH(e) in wild-type hKv1.3, but it was insensitive to pH(e) in H399K and H399L mutants. Based on these observations we expanded our model and propose that the potential barrier created by the protonated histidines impedes the passage of K(+) ions between the extracellular medium and the control site in both directions and the effect on inactivation rate (acceleration or slowing) depends on the relative contribution of filling from the extracellular and intracellular sides.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	European Biophysics Journal. - 37 : 7 (2008), p. 1145-1156. -
További szerzők:	Hajdu Péter (1975-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Panyi György (1966-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító)
Internet cím:	elektronikus változat DOI
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