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001-es BibID:	BIBFORM004837
Első szerző:	Hajas György (biológus)
Cím:	New phenotypic, functional and electrophysiological characteristics of KG-1 cells / György Hajas, Emese Zsiros, Tünde László, Péter Hajdú, Sándor Somodi, Bence Réthi, Péter Gogolák, Katalin Ludányi, György Panyi, Éva Rajnavölgyi
Dátum:	2004
Megjegyzések:	Myeloid dendritic cells (DC) are representatives of a rare and phenotypically diverse population of professional antigen presenting cells possessing high functional heterogeneity and flexibility. Here we studied the phenotypic, functional and electrophysiological characteristics of KG-1 cells, an erythroleukemia model cell line, which shares morphological and physiological similarities with immature and mature myeloid DC. We compared the expression of internalizing receptors and other cell surface molecules, antigen uptake and migration of unstimulated and activated KG-1 cells with the characteristics of immature and mature DC. Unstimulated KG-1 cells were less potent in capturing extracellular materials than immature DC. In contrast to monocyte-derived DC KG-1 cells stimulated by PMA and ionomycin ceased to migrate along the MIP-3beta chemokine gradient despite their high expression of CCR7 chemokine receptor and MDR, a transporter implicated in DC migration. Moreover, we determined the ion channel repertoire of KG-1 cells before and after treatment with PMA and ionomycin by using the patch-clamp technique. We found that both unstimulated and activated KG-1 cells expressed time- and voltage-independent, ChTx sensitive intracellular Ca(2+)-gated potassium conductance suggesting the presence of K(Ca) channels in their membranes. Based on our results we propose that KG-1 cells resemble myeloid DC but also possess unique phenotypic, functional and electrophysiological characteristics.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analogs and derivatives Animals Calcium Cell Line Cell Movement Cells Dendritic Cells Dextrans Fluorescein Fluorescein-5-isothiocyanate Humans Hungary immunology Ionomycin Isoquinolines Leukemia,Erythroblastic,Acute metabolism Patch-Clamp Techniques physiology Potassium Research Support Tumor Cells,Cultured
Megjelenés:	Immunology Letters. - 92 : 1-2 (2004), p. 97-106. -
További szerzők:	Zsíros Emese (1980-) (orvos) László Tünde Hajdu Péter (1975-) (biofizikus) Somodi Sándor (1977-) (belgyógyász) Réthi Bence (1973-) (biológus, immunológus) Gogolák Péter (1968-) (biológus, immunológus) Ludányi Katalin (1975-) (immunológus) Panyi György (1966-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus)
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001-es BibID:	BIBFORM037606
Első szerző:	Hajdu Péter (biofizikus)
Cím:	Answer to the "comment on functional consequences of Kv1.3 ion channel rearrangement into the immunological synapse" by Stefan Bittner et al. [Immunol. Lett. 125 (Aug 15 (2)) (2009) 156-157] / Hajdú, Péter, Szilágyi, Orsolya, Tóth, Ágnes, Krasznai, Zoltán, Pocsai, Krisztina, Panyi, György
Dátum:	2010
ISSN:	0165-2478
Tárgyszavak:	Orvostudományok Elméleti orvostudományok szerkesztői levél egyetemen (Magyarországon) készült közlemény
Megjelenés:	Immunology Letters. - 129 : 1 (2010), p. 47-49. -
További szerzők:	Szilágyi Orsolya (1985-) (molekuláris biológus, biokémikus) Tóth Ágnes (1983-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Pocsai Krisztina (1978-) (élettanász) Panyi György (1966-) (biofizikus)
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001-es BibID:	BIBFORM037605
Első szerző:	Tóth Ágnes (biofizikus)
Cím:	Functional consequences of Kv1.3 ion channel rearrangement into the immunological synapse / Ágnes Tóth, Orsolya Szilágyi, Zoltán Krasznai, György Panyi, Péter Hajdú
Dátum:	2009
ISSN:	0165-2478
Megjegyzések:	Formation of immunological synapse (IS), the interface between T cells and antigen presenting cells, is a crucial step in T cell activation. This conjugation formation results in the rearrangement and segregation of a set of membrane bound and cytosolic proteins, including that of the T cell receptor, into membrane domains. It was showed earlier that Kv1.3, the dominant voltage-gated potassium channel of T cells redistributes into the IS on interaction with its specific APC. In the present experiments we investigated the functional consequences of the translocation of Kv1.3 channels into the IS formed between mouse helper T (T(h)2) and B cells. Biophysical characteristics of whole-cell Kv1.3 current in standalone cells (c) or ones in IS (IS) were determined using voltage-clamp configuration of standard whole-cell patch-clamp technique. Patch-clamp recordings showed that the activation of Kv1.3 current slowed (tau(a,1s) 2.36 +/- 0.13 ms (n = 7): tau(a,c) = 1.36 +/- 0.06 ms (n = 18)) whereas the inactivation rate increased (tau(i,1S) = 263 +/- 29 ms (n = 7): tau(i,c) = 365 +/- 27 ms (n = 17)) in cells being in IS compared to the standalone cells. The equilibrium distribution between the open and the closed states of Kv1.3 (voltage-dependence of steady-state activation) was shifted toward the depolarizing potentials in T cells engaged into IS (V-1/2,V-1S = -20.9 +/- 2 mV (n = 7). V-1/2,V-c = -26.4 +/- 115 mV (n = 12)). Thus, segregation of Kv1.3 channels into the IS modifies the gating properties of the channels. Application of protein kinase (PK) inhibitors (PKC: GF109203X, PKA: H89, p56Lck: damnacanthal) demonstrated that increase in the inactivation rate can be explained by the dephosphorylation of the channel protein. However, the slower activation kinetics of Kv1.3 in IS is likely to be the consequence of the redistribution of the channels into distinct membrane domains.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Cells immunological synapse Kinetics mouse Potassium Proteins egyetemen (Magyarországon) készült közlemény
Megjelenés:	Immunology Letters. - 125 : 1 (2009), p. 15-21. -
További szerzők:	Szilágyi Orsolya (1985-) (molekuláris biológus, biokémikus) Krasznai Zoltán (1950-) (biofizikus) Panyi György (1966-) (biofizikus) Hajdu Péter (1975-) (biofizikus)
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001-es BibID:	BIBFORM014028
Első szerző:	Varga Zoltán (biofizikus, szakfordító)
Cím:	Ion channels in T lymphocytes : an update on facts, mechanisms and therapeutic targeting in autoimmune diseases / Varga Zoltan, Hajdu Peter, Panyi Gyorgy
Dátum:	2010
ISSN:	0165-2478
Megjegyzések:	During the last quarter of a century a large body of evidence was gathered about the involvement ofion channels in T lymphocyte activation. A series of remarkable findings promoted T cell ion channelsto become potential pharmaceutical targets in the therapy of autoimmune disorders. Numerous comprehensivereviews describe the types of ion channels found in the plasma membrane of T cells andtheir roles in signaling pathways leading to activation, the changes in the expression of these channelsbrought upon by differentiation to various T cell subsets, the formation and possible functions of signalingmolecular clusters that include ion channels in the immunological synapse, the discovery and refinementof structurally different ion channel blockers and the successful in vivo application of such compoundsto suppress hypersensitivity reactions and autoimmune processes. In this review we wish to provide aconcise update on these topics from recent years, highlighting the most notable developments.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban T lymphocyte potassium channel autoimmune disease
Megjelenés:	Immunology Letters. - 130 : 1-2 (2010), p. 19-25. -
További szerzők:	Hajdu Péter (1975-) (biofizikus) Panyi György (1966-) (biofizikus)
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