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001-es BibID:	BIBFORM052924
Első szerző:	Bartók Ádám (biotechnológus)
Cím:	Margatoxin is a non-selective inhibitor of human Kv1.3 K+ channels / Adam Bartok, Agnes Toth, Sandor Somodi, Tibor G. Szanto, Peter Hajdu, Gyorgy Panyi, Zoltan Varga
Dátum:	2014
ISSN:	0041-0101
Megjegyzések:	Margatoxin (MgTx), an alpha-KTx scorpion toxin, is considered a selective inhibitor of the Kv1.3 K+ channel. This peptide is widely used in ion channel research; however, a comprehensive study of its selectivity with electrophysiological methods has not been published yet. The lack of selectivity might lead to undesired side effects upon therapeutic application or may lead to incorrect conclusion regarding the role of a particular ion channel in a physiological or pathophysiological response either in vitro or in vivo. Using the patch-clamp technique we characterized the selectivity profile of MgTx using L929 cells expressing mKv1.1 channels, human peripheral lymphocytes expressing Kv1.3 channels and transiently transfected tsA201 cells expressing hKv1.1, hKv1.2, hKv1.3, hKv1.4-IR, hKv1.5, hKv1.6, hKv1.7, rKv2.1, Shaker-IR, hERG, hKCa1.1, hKCa3.1 and hNav1.5 channels. MgTx is indeed a high affinity inhibitor of Kv1.3 (Kd = 11.7 pM) but is not selective, it inhibits the Kv1.2 channel with similar affinity (Kd = 6.4 pM) and Kv1.1 in the nanomolar range (Kd = 4.2 nM). Based on our comprehensive data MgTX has to be considered a non-selective Kv1.3 inhibitor, and thus, experiments aiming at elucidating the significance of Kv1.3 in in vitro or in vivo physiological responses have to be carefully evaluated.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban ion channel potassium channel scorpion toxin margatoxin
Megjelenés:	Toxicon. - 87 (2014), p. 6-16. -
További szerzők:	Tóth Ágnes (1983-) (biofizikus) Somodi Sándor (1977-) (belgyógyász) Szántó Gábor Tibor (1980-) (vegyész) Hajdu Péter (1975-) (biofizikus) Panyi György (1966-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM091018
Első szerző:	Tajti Gábor (gyógyszerész, biofizikus, sejtbiológus)
Cím:	Immunomagnetic separation is a suitable method for electrophysiology and ion channel pharmacology studies on T cells / Gabor Tajti, Tibor Gabor Szanto, Agota Csoti, Greta Racz, César Evaristo, Peter Hajdu, Gyorgy Panyi
Dátum:	2021
ISSN:	1933-6950 1933-6969
Megjegyzések:	Ion channels play pivotal role in the physiological and pathological function of immune cells. As immune cells represent a functionally diverse population, subtype-specific functional studies, such as single-cell electrophysiology require proper subset identification and separation. Magneticactivated cell sorting (MACS) techniques provide an alternative to fluorescence-activated cell sorting (FACS), however, the potential impact of MACS-related beads on the biophysical and pharmacological properties of the ion channels were not studied yet. We studied the aforementioned properties of the voltage-gated Kv1.3 K+ channel in activated CD4+ T-cells as well as the membrane capacitance using whole-cell patch-clamp following immunomagnetic positive separation, using the REAlease? kit. This kit allows three experimental configurations: bead-bound configuration, bead-free configuration following the removal of magnetic beads, and the labelfree configuration following removal of CD4 recognizing antibody fragments. As controls, we used FACS separation as well as immunomagnetic negative selection. The membrane capacitance and of the biophysical parameters of Kv1.3 gating, voltage-dependence of steady-state activation and inactivation kinetics of the current were not affected by the presence of MACS-related compounds on the cell surface. We found subtle differences in the activation kinetics of the Kv1.3 current that could not be explained by the presence of MACS-related compounds. Neither the equilibrium block of Kv1.3 by TEA+ or charybdotoxin (ChTx) nor the kinetics of ChTx block are affected by the presence of the magnetics beads on the cell surface. Based on our results MACS is a suitable method to separate cells for studying ion channels in non-excitable cells, such as T-lymphocytes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Kv1.3 cd4+ T-cell magneticactivated cell sorting macs immunomagnetic separation fluorescenceactivated cell sorting facs
Megjelenés:	Channels. - 15 : 1 (2021), p. 53-66. -
További szerzők:	Szántó Gábor Tibor (1980-) (vegyész) Csóti Ágota (1989-) (biológus) Rácz Gréta Evaristo, César Hajdu Péter (1975-) (biofizikus) Panyi György (1966-) (biofizikus)
Pályázati támogatás:	OTKA K119417 Egyéb EFOP-3.6.2-16-2017-00006 EFOP GINOP-2.3.2-15-2016-00015 GINOP NKFIH K128525 Egyéb Bolyai Research Fellowship Egyéb
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