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001-es BibID:	BIBFORM057465
Első szerző:	Kappelmayer János (laboratóriumi szakorvos)
Cím:	Thrombocyta/megakaryocyta markerek áramlási citometriai alkalmazhatósága / Kappelmayer János, Kiss Csongor, Udvardy Miklós, Csáthy László, Kiss Flóra, Simon Ágnes, Hevessy Zsuzsanna
Dátum:	2013
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idézhető absztrakt
Megjelenés:	Hematológia-Transzfuziológia. Supplementum. - 46 : Suppl. 1 (2013), p. 73. -
További szerzők:	Kiss Csongor (1956-) (hematológus, onkológus) Udvardy Miklós (1947-) (belgyógyász, haematológus) Csáthy László (1979-) (laboratóriumi szakorvos) Kiss Flóra (1980-) (bőrgyógyász) Simon Ágnes (1969-) (laboratóriumi szakorvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM034571
Első szerző:	Kappelmayer János (laboratóriumi szakorvos)
Cím:	Progress in defining multidrug resistance in leukemia / Kappelmayer János, Simon Ágnes, Kiss Flóra, Hevessy Zsuzsa
Dátum:	2004
ISSN:	1473-7159
Megjegyzések:	Multidrug resistance (MDR) is a naturally occurring defense phenomenon by which cells battle against chemically foreign substances (xenobiotics), including some cytotoxic drugs. Membrane transporter hyperactivity is a major contributor to MDR and is the primary target of both diagnostic and therapeutic interventions. Multi-xenobiotic resistance can be exploited as several fluorescent indicator probes are extruded by the same drug transporters, making it possible to quantitatively measure MDR activity in cell lines and clinical samples by flow cytometry. The literature on MDR is reported in a number of different formats, making it difficult to compare data from various groups. This article will briefly review the pathomechanism, then focus upon the diagnostic approach, the interpretation of results from clinical samples and correlations with other variables. The authors believe that a standardized MDR assay, as well as a suitable monitoring test, may become a prognostic marker in several types of leukemia.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Expert Review Of Molecular Diagnostics. - 4 : 2 (2004), p. 209-217. -
További szerzők:	Simon Ágnes (1969-) (laboratóriumi szakorvos) Kiss Flóra (1980-) (bőrgyógyász) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos)
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001-es BibID:	BIBFORM005605
Első szerző:	Kiss Flóra (bőrgyógyász)
Cím:	A coagulation factor becomes useful in the study of acute leukemias : studies with blood coagulation factor XIII / Kiss F., Simon Á., Csáthy L., Hevessy Z., Katona É., Kiss C., Kappelmayer J.
Dátum:	2008
Megjegyzések:	The intracellular form of the coagulation factor XIII has previously been identified by immunomorphological techniques using polyclonal antibodies. In these studies, only the A subunit (FXIII-A) was detectable in megakaryocytes/platelets and in monocytes/ macrophages. We developed several novel monoclonal antibody clones directed to both subunits (FXIII-A and FXIII-B) and investigated their appearance in normal and leukemic cells. By using 3- and 4-color flow cytometry FXIII expression was investigated in normal peripheral blood and bone marrow samples and in acute myeloblastic (AML) and lymphoblastic (ALL) leukemia cases. Samples were studied by Western blotting and confocal laser scanning microscopy. With a previously published ELISA assay applying two monoclonal antibodies directed to different epitopes in FXIII-A, we were able to measure the intracytoplasmic content of FXIII-A in normal cells and leukemic blasts. FXIII-A was detectable in normal peripheral blood monocytes and in large quantities in platelets, but both cell types were negative for FXIII-B. There was no surface staining for FXIII-A, it only appeared intracellularly. In samples derived from patients with AML M4 and M5, FXIII-A sensitively identified blast cells. Although normal lymphocytes do not express FXIII-A, 40% of ALL cases showed significant FXIII-A expression as determined by flow cytometry. FXIII-A positivity of lymphoblasts was verified by Western blotting, ELISA, and confocal laser scanning microscopy cytometry. These data provide evidence that FXIII-A is a sufficiently sensitive marker in differentiating myeloblasts and monoblasts and is suitable for identifying leukemia-associated phenotypes in ALL.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban factor XIII flow cytometry acute leukemia phenotype
Megjelenés:	Cytometry. Part A. - 73A : 3 (2008), p. 194-201. -
További szerzők:	Simon Ágnes (1969-) (laboratóriumi szakorvos) Csáthy László (1979-) (laboratóriumi szakorvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Katona Éva (1961-) (klinikai biokémikus) Kiss Csongor (1956-) (hematológus, onkológus) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:	DOI elektronikus változat
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001-es BibID:	BIBFORM034576
Első szerző:	Simon Ágnes (laboratóriumi szakorvos)
Cím:	Expression of coagulation factor XIII subunit A in acute promyelocytic leukemia / Ágnes Simon, Zsuzsa Bagoly, Zsuzsanna Hevessy, László Csáthy, Éva Katona, György Vereb, Anikó Ujfalusi, László Szerafin, László Muszbek, János Kappelmayer
Dátum:	2012
ISSN:	1552-4949
Megjegyzések:	Leukemic cells often express markers which are not characteristic of their particular cell lineage. In this study we identified the "A" subunit of coagulation factor XIII (FXIII-A) in leukemic promyelocytes in de novo AML M3 cases. The cytoplasmic presence of factor XIII-A has previously been shown only in platelets/megakaryocytes and monocytes/macrophages. Furthermore, more recently we described the presence of FXIII-A in leukemic lymphoblasts. We studied 14 patients with this rare type of acute leukemia in a period of 4 years and investigated their bone marrow samples by 3-color flow cytometry upon diagnosis, mainly focusing on FXIII-A expression of leukemic cells. We detected FXIII-A also by ELISA, Western-blot and confocal laser scanning microscopy. This was a homogenous group of AML M3 patients with translocation t(15;17)(q22;q21) detected by fluorescence in situ hybridization (FISH). In 10 out of 14 samples, FXIII-A was detectable by flow cytometry and was coexpressed with markers characteristic for leukemic promyleocytes (CD45dim/CD13+/CD33+/CD117+/cyMPO+ and HLA-DR-/CD34-/CD14-/CD15-). Staining for the markers GPIIb and GPIX were negative, and FXIII-A was identified in the cytoplasm of the cells by confocal microscopy in a relatively high quantity, as measured by ELISA. By Western blot analysis we could identify FXIII-A in the native 82 kD form and in cleaved forms corresponding to cleavage products observed when purified FXIII-A was treated by human neutrophil elastase. Since normal promyelocytes were FXIII-A negative, this novel expression site of FXIII-A in AML M3 can be considered as a leukemia associated immunophenotype and may have pathophysiological significance.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény Molekuláris Medicina
Megjelenés:	Cytometry. Part B. Clinical Cytometry. - 82B : 4 (2012), p. 209-216. -
További szerzők:	Bagoly Zsuzsa (1978-) (orvos) Hevessy Zsuzsanna (1966-) (laboratóriumi szakorvos) Csáthy László (1979-) (laboratóriumi szakorvos) Katona Éva (1961-) (klinikai biokémikus) Vereb György (1965-) (biofizikus, orvos) Ujfalusi Anikó (1968-) (gyermekorvos, laboratóriumi szakorvos) Szerafin László (1958-) (belgyógyászat, haematológia, klinikai onkológia szakorvos) Muszbek László (1942-) (haematológus, kutató orvos) Kappelmayer János (1960-) (laboratóriumi szakorvos)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Celluláris hematológia - immunológia TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP A véralvadás XIII-as faktorának (FXIII) struktúrája, funkciója, előfordulása egyéb testnedvekben és kapcsolata trombotikus megbetegedésekkel
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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