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1.

001-es BibID:BIBFORM055866
Első szerző:Bácsi Attila (immunológus)
Cím:Reactive Oxygen Species Generated by Pollen Grain's NAD(P)H Oxidase Augment Allergic Ocular Symptoms / Bácsi Attila, Dharajiya Nilesh, Choudhury Barun K., Sur Sanjiv, Boldogh István
Dátum:2004
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Journal of Allergy and Clinical Immunology. - 113 : Suppl. 2. (2004), p. S164. -
További szerzők:Dharajiya, Nilesh G. Choudhury, Barun K. Sur, Sanjiv Boldogh István
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2.

001-es BibID:BIBFORM055871
Első szerző:Bácsi Attila (immunológus)
Cím:Antigen-independent mitochondria-driven release of biogenic amines from RBL-2H3 cells / Bácsi Attila, Chodaczek Grzegorz, Dharajiya Nilesh, Sur Sanjiv, Hazra Tapas K., Boldogh István
Dátum:2007
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Journal of Allergy and Clinical Immunology. - 123 (2007), p. S118. -
További szerzők:Chodaczek, Grzegorz Dharajiya, Nilesh G. Sur, Sanjiv Hazra, Tapas K. Boldogh István
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3.

001-es BibID:BIBFORM047645
035-os BibID:PMID:16210058
Első szerző:Bácsi Attila (immunológus)
Cím:Effect of pollen-mediated oxidative stress on immediate hypersensitivity reactions and late-phase inflammation in allergic conjunctivitis / Attila Bacsi, Nilesh Dharajiya, Barun K. Choudhury, Sanjiv Sur, Istvan Boldogh
Dátum:2005
ISSN:0091-6749
Megjegyzések:BACKGROUND:Allergic eye diseases are complex inflammatory conditions of the conjunctiva that are becoming increasingly prevalent and present an increasing economic burden because of direct and indirect health expenditures.OBJECTIVE:We sought to identify factors that may synergize with antigen-induced allergic inflammation and lead to allergic conjunctivitis. We used a murine model of allergic conjunctivitis to test the effect of oxidative stress generated by pollen oxidases using nicotinamide adenine dinucleotide (reduced) or nicotinamide adenine dinucleotide phosphate (reduced) (NAD[P]H) as an electron donor present in pollen grains.METHODS:Reactive oxygen species (ROS) generation by hydrated Ambrosia artemisiifolia pollen (short ragweed pollen; RWP) grains was determined by using 2'-7'-dihydro-dichlorofluorescein diacetate, nitroblue tetrazolium reduction, and Amplex Red assay. The RWP-induced changes in intracellular ROS levels were examined in A549 cells, human primary bronchial epithelial cells, and murine conjunctiva.RESULTS:Ragweed pollen grains contain NAD(P)H oxidase activity, which is diphenyleneiodonium-sensitive and quinacrine-sensitive and sodium azide-resistant. These NAD(P)H oxidases generate a superoxide anion that can be converted to H2O2 by pollen grain-associated superoxide dismutase. These diffusible oxygen radicals from pollen grains increase intracellular ROS levels in cultured epithelial cells and murine conjunctiva. Similar phenomena were observed in sensitized and naive mice, indicating that the RWP-induced oxidative stress in conjunctival epithelium is independent of adaptive immunity. Inactivation of NAD(P)H oxidase activity in RWP decreases the immediate-type hypersensitivity and inflammatory cell infiltration into the conjunctiva.CONCLUSION:Our data suggest that ROS generated by NAD(P)H oxidases in pollen grains intensify immediate allergic reactions and recruitment of inflammatory cells in murine conjunctiva.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Pollen NAD(P)H oxidase
oxidative stress
epithelium
conjunctivitis
Megjelenés:Journal of Allergy and Clinical Immunology. - 116 : 4 (2005), p. 836-843. -
További szerzők:Dharajiya, Nilesh G. Choudhury, Barun K. Sur, Sanjiv Boldogh István
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4.

001-es BibID:BIBFORM038720
Első szerző:Bácsi Attila (immunológus)
Cím:Subpollen particles : carriers of allergenic proteins and oxidases / Bacsi A., Choudhury B.K., Dharajiya N., Sur S., Boldogh I.
Dátum:2006
ISSN:0091-6749
Megjegyzések:Pollen is known to induce allergic asthma in atopic individuals, although only a few inhaled pollen grains penetrate into the lower respiratory tract. OBJECTIVE: We sought to provide evidence that subpollen particles (SPPs) of respirable size, possessing both antigenic and redox properties, are released from weed pollen grains and to test their role in allergic airway inflammation. METHODS: The release of SPPs was analyzed by means of microscopic imaging and flow cytometry. The redox properties of SPPs and the SPP-mediated oxidative effect on epithelial cells were determined by using redox-sensitive probes and specific inhibitors. Western blotting and amino acid sequence analysis were used to examine the protein components of the SPP. The allergenic properties of the SPP were determined in a murine model of experimental asthma. RESULTS: Ragweed pollen grains released 0.5 to 4.5 microm of SPPs on hydration. These contained Amb a 1, along with other allergenic proteins of ragweed pollen, and possessed nicotinamide adenine dinucleotide (reduced) or nicotinamide adenine dinucleotide phosphate (reduced) [NAD(P)H] oxidase activity. The SPPs significantly increased the levels of reactive oxygen species (ROS) in cultured cells and induced allergic airway inflammation in the experimental animals. Pretreatment of the SPPs with NAD(P)H oxidase inhibitors attenuated their capacity to increase ROS levels in the airway epithelial cells and subsequent airway inflammation. CONCLUSIONS: The allergenic potency of SPPs released from ragweed pollen grains is mediated in tandem by ROS generated by intrinsic NAD(P)H oxidases and antigenic proteins.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Allergy And Clinical Immunology. - 118 : 4 (2006), p. 844-850. -
További szerzők:Choudhury, Barun K. Dharajiya, Nilesh G. Sur, Sanjiv Boldogh István
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5.

001-es BibID:BIBFORM055867
Első szerző:Boldogh István
Cím:Oxidative Stress Generated by Intrinsic Pollen NADPH Oxidases Is Required for Inducing Allergic Airway Inflammation / Boldogh István, Bácsi Attila, Choudhury Barun K., Dharajiya Nilesh, Alam Rafeul, Sur Sanjiv
Dátum:2004
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Journal of Allergy and Clinical Immunology. - 113 (2004), p. S164. -
További szerzők:Bácsi Attila (1967-) (immunológus) Choudhury, Barun K. Dharajiya, Nilesh G. Alam, Rafeul Sur, Sanjiv
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6.

001-es BibID:BIBFORM047644
Első szerző:Boldogh István
Cím:ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation / Istvan Boldogh, Attila Bacsi, Barun K. Choudhury, Nilesh Dharajiya, Rafeul Alam, Tapas K. Hazra, Sankar Mitra, Randall M. Goldblum, Sanjiv Sur
Dátum:2005
ISSN:0021-9738
Megjegyzések:Pollen exposure induces allergic airway inflammation in sensitized subjects. The role of antigenic pollen proteinsin the induction of allergic airway inflammation is well characterized, but the contribution of otherconstituents in pollen grains to this process is unknown. Here we show that pollen grains and their extractscontain intrinsic NADPH oxidases. The pollen NADPH oxidases rapidly increased the levels of ROS in lungepithelium as well as the amount of oxidized glutathione (GSSG) and 4-hydroxynonenal (4-HNE) in airwayliningfluid. These oxidases, as well as products of oxidative stress (such as GSSG and 4-HNE) generated bythese enzymes, induced neutrophil recruitment to the airways independent of the adaptive immune response.Removal of pollen NADPH oxidase activity from the challenge material reduced antigen-induced allergic airwayinflammation, the number of mucin-containing cells in airway epithelium, and antigen-specific IgE levelsin sensitized mice. Furthermore, challenge with Amb a 1, the major antigen in ragweed pollen extract that doesnot possess NADPH oxidase activity, induced low-grade allergic airway inflammation. Addition of GSSG or4-HNE to Amb a 1 challenge material boosted allergic airway inflammation. We propose that oxidative stressgenerated by pollen NADPH oxidases (signal 1) augments allergic airway inflammation induced by pollenantigen (signal 2).
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
DNA double strand breaks
Megjelenés:Journal of Clinical Investigation. - 115 : 8 (2005), p. 2169-2179. -
További szerzők:Bácsi Attila (1967-) (immunológus) Choudhury, Barun K. Dharajiya, Nilesh G. Alam, Rafeul Hazra, Tapas K. Mitra, Sankar Goldblum, Randall M. Sur, Sanjiv
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7.

001-es BibID:BIBFORM010266
Első szerző:Chodaczek, Grzegorz
Cím:Ragweed pollen-mediated IgE-independent release of biogenic amines from mast cells via induction of mitochondrial dysfunction / Grzegorz Chodaczek, Attila Bácsi, Nilesh G. Dharajiya, Sanjiv Sur, Tapas K. Hazra, István Boldogh
Dátum:2009
Megjegyzések:Normal functions of mitochondria are required for physiological dynamics of cells, while their dysfunction contributes to development of various disorders including those of immune system. Here we demonstrate that exposure of mast cells to ragweed pollen extract increases production of H2O2 via mitochondrial respiratory complex III. These mitochondrial ROS (mtROS) enhance secretion of histamine and serotonin from mast cells, but not enzymes such as ?-hexosaminidase, independently from FcRI-generated stimuli. The release of biogenic amines is associated with inhibition of secretory granules' H+-ATPase activity, activation of PKC-? and microtubule-dependent motility, and it is independent from intracellular free Ca2+ levels. To asses differences from IgE-mediated mast cell degranulation we show that mtROS decrease antigen-triggered ?-hexosaminidase release, while they are synergistic with antigen-induced IL-4 production in sensitized cells. Taken together, these data indicate that mitochondrial dysfunction can act independently from adaptive immunity, as well as augments Th2-type responses. Pharmacological maintenance of physiological mitochondrial function could have clinical benefits in prevention and treatment of allergic diseases.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Mast cells
Mitochondria
Degranulation
Reactive oxygen species
Megjelenés:Molecular Immunology. - 46 : 13 (2009), p. 2505-2514. -
További szerzők:Bácsi Attila (1967-) (immunológus) Dharajiya, Nilesh G. Sur, Sanjiv Hazra, Tapas K. Boldogh István
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8.

001-es BibID:BIBFORM055865
Első szerző:Choudhury, Barun K.
Cím:Stimulation of Innate Immune Response Induces Long Term Allergic Sensitization and Allergic Airway Inflammation Independent of STAT-6 / Choudhury Barun K., Wild James S., Dharajiya Nilesh, Bácsi Attila, Alam Rafeul, Boldogh István, Sur Sanjiv
Dátum:2004
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Journal of Allergy and Clinical Immunology. - 113 : Suppl. 2. (2004), p. S163. -
További szerzők:Wild, James R. Dharajiya, Nilesh G. Bácsi Attila (1967-) (immunológus) Alam, Rafeul Boldogh István Sur, Sanjiv
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9.

001-es BibID:BIBFORM055869
Első szerző:Dharajiya, Nilesh G.
Cím:Inhibiting pollen NADPH oxidase-induced signal by intrapulmonary administration of antioxidants blocks allergic airway inflammation / Dharajiya Nilesh, Choudhury Barun K., Bácsi Attila, Boldogh István, Alam Rafeul, Sur Sanjiv
Dátum:2007
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Journal of Allergy and Clinical Immunology. - 119 (2007), p. S128. -
További szerzők:Choudhury, Barun K. Bácsi Attila (1967-) (immunológus) Boldogh István Alam, Rafeul Sur, Sanjiv
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10.

001-es BibID:BIBFORM002000
Első szerző:Dharajiya, Nilesh G.
Cím:Inhibiting pollen reduced nicotinamide adenine dinucleotide phosphate oxidase-induced signal by intrapulmonary administration of antioxidants blocks allergic airway inflammation / Nilesh Dharajiya, Barun K. Choudhury, Attila Bacsi, Istvan Boldogh, Rafeul Alam, Sanjiv Sur
Dátum:2007
Megjegyzések:Ragweed extract (RWE) contains NADPH oxidases that induce oxidative stress in the airways independent of adaptive immunity (signal 1) and augment antigen (signal 2)-induced allergic airway inflammation. OBJECTIVE: To test whether inhibiting signal 1 by administering antioxidants inhibits allergic airway inflammation in mice. METHODS: The ability of ascorbic acid (AA), N-acetyl cystenine (NAC), and tocopherol to scavenge pollen NADPH oxidase-generated reactive oxygen species (ROS) was measured. These antioxidants were administered locally to inhibit signal 1 in the airways of RWE-sensitized mice. Recruitment of inflammatory cells, mucin production, calcium-activated chloride channel 3, IL-4, and IL-13 mRNA expression was quantified in the lungs. RESULTS: Antioxidants inhibited ROS generation by pollen NADPH oxidases and intracellular ROS generation in cultured epithelial cells. AA in combination with NAC or Tocopherol decreased RWE-induced ROS levels in cultured bronchial epithelial cells. Coadministration of antioxidants with RWE challenge inhibited 4-hydroxynonenal adduct formation, upregulation of Clca3 and IL-4 in lungs, mucin production, recruitment of eosinophils, and total inflammatory cells into the airways. Administration of antioxidants with a second RWE challenge also inhibited airway inflammation. However, administration of AA+NAC 4 or 24 hours after RWE challenge failed to inhibit allergic inflammation. CONCLUSION: Signal 1 plays a proinflammatory role during repeated exposure to pollen extract. We propose that inhibiting signal 1 by increasing antioxidant potential in the airways may be a novel therapeutic strategy to attenuate pollen-induced allergic airway inflammation. CLINICAL IMPLICATIONS: Administration of antioxidants in the airways may constitute a novel therapeutic strategy to prevent pollen induced allergic airway inflammation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Allergy
inflammation
antioxidants
eosinophils
TH2
Megjelenés:The Journal of allergy and clinical immunology 119 : 3 (2007), p. 646-653. -
További szerzők:Choudhury, Barun K. Bácsi Attila (1967-) (immunológus) Boldogh István Alam, Rafeul Sur, Sanjiv
Internet cím:elektronikus változat
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elektronikus változat
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11.

001-es BibID:BIBFORM001998
Első szerző:Dharajiya, Nilesh G.
Cím:Pollen NAD(P)H Oxidases and Their Contribution to Allergic Inflammation / Nilesh G. Dharajiya, Attila Bacsi, Istvan Boldogh, Sanjiv Sur
Dátum:2007
Megjegyzések:This article provides an overview of NADPH oxidase and its role in allergic inflammation. A background and historical perspectives of NADPH oxidase are first provided, followed by a detailed overview of mammalian NADPH oxidase subunits and their functional organization. Plant NADPH oxidase, the authors' discovery of NADPH oxidase in pollens, and their contribution to allergic inflammation are then discussed, concluding with a discussion of future directions and outstanding questions that require attention.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Pollen
NADPH oxidase
Allergy
Megjelenés:Immunology and Allergy Clinics of North America 27 : 1 (2007), p. 45-63. -
További szerzők:Bácsi Attila (1967-) (immunológus) Boldogh István Sur, Sanjiv
Internet cím:elektronikus változat
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elektronikus változat
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12.

001-es BibID:BIBFORM041840
Első szerző:Murai, Hiroki
Cím:Alternaria-Induced Release of IL-18 from Damaged Airway Epithelial Cells : n NF-kB Dependent Mechanism of Th2 Differentiation? / Hiroki Murai, Huibin Qi, Barun Choudhury, Jim Wild, Nilesh Dharajiya, Swapnil Vaidya, Anjana Kalita, Attila Bacsi, David Corry, Alexander Kurosky, Allan Brasier, Istvan Boldogh, Sanjiv Sur
Dátum:2012
Megjegyzések:BACKGROUND: A series of epidemiologic studies have identified the fungus Alternaria as a major risk factor for asthma. The airway epithelium plays a critical role in the pathogenesis of allergic asthma. These reports suggest that activated airway epithelial cells can produce cytokines such as IL-25, TSLP and IL-33 that induce Th2 phenotype. However the epithelium-derived products that mediate the pro-asthma effects of Alternaria are not well characterized. We hypothesized that exposure of the airway epithelium to Alternaria releasing cytokines that can induce Th2 differentiation.METHODOLOGY/PRINCIPAL FINDING: We used ELISA to measure human and mouse cytokines. Alternaria extract (ALT-E) induced rapid release of IL-18, but not IL-4, IL-9, IL-13, IL-25, IL-33, or TSLP from cultured normal human bronchial epithelial cells; and in the BAL fluids of naïve mice after challenge with ALT-E. Both microscopic and FACS indicated that this release was associated with necrosis of epithelial cells. ALT-E induced much greater IL-18 release compared to 19 major outdoor allergens. Culture of naïve CD4 cells with rmIL-18 induced Th2 differentiation in the absence of IL-4 and STAT6, and this effect was abrogated by disrupting NF- ?B p50 or with a NEMO binding peptide inhibitor.CONCLUSION/SIGNIFICANCE: Rapid and specific release of IL-18 from Alternaria-exposed damaged airway epithelial cells can directly initiate Th2 differentiation of naïve CD4(+) T-cells via a unique NF-?B dependent pathway.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:PLoS One. - 7 : 2 (2012), p. e30280. -
További szerzők:Qi, Huibin Choudhury, Barun K. Wild, Jim Dharajiya, Nilesh G. Vaidya, Swapnil Kalita, Anjana Bácsi Attila (1967-) (immunológus) Corry, David Kurosky, Alexander Brasier, Allan R. Boldogh István Sur, Sanjiv
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