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001-es BibID:	BIBFORM104602
035-os BibID:	(Scopus)85141858760 (WOS)000894561800002
Első szerző:	Bácsi Attila (immunológus)
Cím:	Whole blood transcriptome characterization of young female triathlon athletes following an endurance exercise : a pilot study / Bácsi Attila, Penyige András, Becs Gergely, Benkő Szilvia, Kovács Elek Gergő, Jenei Csaba, Pócsi István, Balla József, Csernoch László, Balatoni Ildikó
Dátum:	2022
ISSN:	1094-8341
Megjegyzések:	The vast majority of studies focusing on the effects of endurance exercise on hematological parameters and leukocyte gene expression were performed in adult men, so our aim was to investigate these changes in young females. Four young (age 15.3 ? 1.3 years) elite female athletes completed an exercise session, in which they accomplished the cycling and running disciplines of a junior triathlon race. Blood samples were taken immediately before the exercise, right after the exercise, and then 1, 2, and 7 days later. Analysis of cell counts and routine biochemical parameters was complemented by RNA sequencing (RNA-seq) to whole blood samples. The applied exercise load did not trigger remarkable changes in either cardiovascular or biochemical parameters; however, it caused a significant increase in the percentage of neutrophils and a significant reduction in the ratio of lymphocytes immediately after exercise. Furthermore, endurance exercise induced a characteristic gene expression pattern change in the blood transcriptome. Gene set enrichment analysis (GSEA) using the Reactome database revealed that the expression of genes involved in immune processes and neutrophil granulocyte activation was upregulated, while the expression of genes important in translation and rRNA metabolism was downregulated. Comparison of a set of immune cell gene signatures (ImSig) and our transcriptomic data identified 15 overlapping genes related to T cell functions, and involved in podosome formation and adhesion to the vessel wall. Our results suggest that RNA-seq to whole blood together with ImSig analysis are useful tools for investigation of systemic responses to endurance exercise.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk ImSig RNA sequencing endurance exercise whole blood transcriptome young female athletes
Megjelenés:	Physiological Genomics. - 54 : 11 (2022), p. 457-469. -
További szerzők:	Penyige András (1954-) (molekuláris genetikus) Becs Gergely Benkő Szilvia (1973-) (molekuláris biológus) Kovács Elek Gergő Jenei Csaba (1976-) (kardiológus) Pócsi István (1961-) (vegyész) Balla József (1959-) (belgyógyász, nephrológus) Csernoch László (1961-) (élettanász) Balatoni Ildikó (1970-) (orvos)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00062 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM040795
Első szerző:	Varga Alíz (immunológus)
Cím:	Ragweed pollen extract intensifies LPS-induced priming of NLRP3 inflammasome in human macrophages / Varga Aliz, Budai Marietta, Milesz Sándor, Bácsi Attila, Tőzsér József, Benkő Szilvia
Dátum:	2013
ISSN:	0019-2805
Megjegyzések:	Ragweed pollen extract (RWE) has been described to possess intrinsic NADPH oxidase activity that induces oxidative stress by initiating the production of intracellular reactive oxygen species (ROS). ROS are important contributors to the manifestation of the allergic inflammation, furthermore, concomitant exposure to an allergen and an endotoxin trigger a stronger inflammatory response. One of the main proinflammatory cytokines produced in inflammatory responses is IL-1beta, and its production is associated with caspase-1-containing inflammasome complexes. Intracellular ROS have been implicated in NLRP3 inflammasome-mediated IL-1beta production, therefore, we aimed to study whether RWE influences the function of NLRP3 inflammasome. Here we describe that, in the presence of NADPH, RWE significantly elevates LPS-induced IL-1beta production of THP-1 cells as well as human primary macrophages and dendritic cells. We also demonstrate that increased IL-1beta production is mediated via NLRP3 inflammasome in THP-1 macrophages. We provide evidences that RWE elevates cytosolic ROS level in these cells, and ROS inhibitors abolish IL-1beta production. Furthermore, we show that RWE enhances LPS-induced gene transcription/expression of proIL-1beta and key components of the inflammasome via ROS-dependent mechanism
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Molekuláris Sejt- és Immunbiológiai Doktori Iskola
Megjelenés:	Immunology. - 138 : 4 (2013), p. 392-401. -
További szerzők:	Budai Marietta Margit (1985-) (molekuláris biológus) Milesz Sándor Bácsi Attila (1967-) (immunológus) Tőzsér József (1959-) (molekuláris biológus, biokémikus, vegyész) Benkő Szilvia (1973-) (molekuláris biológus)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Retrovírusok életciklusának vizsgálata különös tekintettel a HIV-2 vírusra TÁMOP-4.2.2.A-11/1/KONV-2012-0023-"VÉD-ELEM" TÁMOP TÁMOP-4.2.2/B-10/1-2010-0024 TÁMOP cMolekuláris Sejt- és Immunbiológiai Doktori Iskola K-73347 OTKA
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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