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001-es BibID:BIBFORM033737
Első szerző:Kováts E.
Cím:Potentiation of HIV envelope glycoprotein and other immunogens by endotoxin (ET) and its molecular fragments / Kovats E., Somlyo B., Csanky E., Shi X. M., Zhang Y. L., Coughlin R. T., Nowotny A.
Dátum:1992
Megjegyzések:The structural requirements for the immunopotentiating (adjuvant) effect of endotoxin (ET) were investigated. Mild hydrolysis (0.2 N acetic acid at 95 degrees C) was applied to various ET preparations and the lipid rich (Lipid A) and polysaccharide-rich (PS) preparations obtained were tested as adjuvants on three immunogens: sheep red blood cells (SRBC), L-glutamine: L-lysine: L-alanine containing random synthetic polypeptide (GLA-40), and recombinant HIV viral envelope polypeptide (CBre3). It was found that not only the Lipid A precipitates, but under certain hydrolytic conditions the non-toxic PS preparations were also potent adjuvants. The exact conditions of hydrolysis which led to the isolation of immune adjuvant bacterial products were established. These materials were also tested for endotoxicity (Limulus lysate clotting, chick embryo lethality and local Shwartzman skin reactivity), as well as for TNF generating activities. It was found that TNF generation runs parallel with toxicity of the samples, but it does not follow the adjuvant activity of the isolates. Chemical analysis of the preparations indicated that they did not contain residual ET or Lipid A, however, they did not exclude that deacylated and dephosphorylated skeletal remains of ET are among those components in these preparations which have immunomodulatory activity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
endotoxin
Megjelenés:International Journal of Immunopharmacology. - 14 : 4 (1992), p. 573-581. -
További szerzők:Somlyó B. Csánky Eszter (1959-) (tüdőgyógyász, klinikai immunológus, allergológus) Shi, X. Zhang, Y. Coughlin, R. T. Nowotny, A.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:BIBFORM033736
Első szerző:Somlyó B.
Cím:Molecular requirements of endotoxin actions, changes the immune adjuvant, TNF liberating and toxic properties of endotoxin during alkaline hydrolysis / Somlyo B., Csanky E., Shi X. M., Zhang Y. L., Kovats E., Bona-Liptak E., Nowotny A. M., Tripodi D., Nowotny A.
Dátum:1992
Megjegyzések:The effect of alkaline pH and alkaline hydrolysis on the physico-chemical and biological properties of endotoxin (ET) isolated from Serratia marcescens ATCC 13477 by the Biovin procedure was studied. Major emphasis was put on the ion exchange column chromatography and immune adjuvant activity (ADA) of the alkali treated samples. To measure changes in some endotoxicity parameters, Limulus lysate clotting (LAL), chick embryo lethality, Shwartzman skin reactivity and in vitro TNF release were measured. The toxic properties of ET, with the unique exception of the Shwartzman skin reactivity, rapidly diminished during alkaline treatment. As immunogen CBre3, a recombinant HIV glycoprotein which spans the C terminus of gp 120 and the N terminus of gp 41, was used in CD-1 mice, alkali treated and immediately neutralized ET samples (zero time) were inactive as adjuvants, in some cases immunosuppression could be clearly seen. But if the alkaline hydrolysis was continued for 6 h, the ADA became higher than it had been for the starting ET sample. Further alkaline hydrolysis eliminated the ADA of the samples. Both NaOH and propylamine acted similarly on the ET preparation. Reaction kinetic studies of the NaOH detoxification indicated the cleavage of ester bound acyl groups with low binding energy. Chemical analyses of the samples revealed that changes occurred in the fatty acid composition, characterized by a loss of approximately half of the 3-OH myristic acid content.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
endotoxin
Megjelenés:International Journal of Immunopharmacology. - 14 : 2 (1992), p. 131-142. -
További szerzők:Csánky Eszter (1959-) (tüdőgyógyász, klinikai immunológus, allergológus) Shi, X. Zhang, Y. Kováts E. Bona-Lipták, E. Nowotny, A. M. Tripodi, D. Nowotny, A.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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