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001-es BibID:	BIBFORM077849
035-os BibID:	(PMID)29457870
Első szerző:	Járvás Gábor (vegyészmérnök)
Cím:	Effect of the flow profile on separation efficiency in pressure-assisted reversed-polarity capillary zone electrophoresis of anions : simulation and experimental evaluation / Gabor Jarvas, Marton Szigeti, Andras Guttman
Dátum:	2018
ISSN:	1615-9306
Megjegyzések:	Capillary electrophoresis connected to electrospray ionization mass spectrometry is a promising combination to analyze complex biological samples. The use of sheathless electrospray ionization interfaces, such as a porous nanoelectrospray capillary emitter, requires the application of forward flow (either by pressure or electroosmosis) to maintain the electrospray process. The analysis of solute molecules with strong negative charges (e.g., aminopyrenetrisulfonate labeled glycans) necessitates a reversedpolarity capillary electrophoresis separation mode, in which case the electroosmotic flow is counter current, thus pressure assistance is necessary. In this study, we compared the effect of forced convection with and without counter electroosmotic flow on the resulting separation efficiency in capillary electrophoresis based on flow profile simulations by computational fluid dynamics technique and by actual experiments. The efficiencies of the detected peaks were calculated from the resulting electropherograms and found approximately 950 000 plates/m for electrophoresis with counter electroosmotic flow, 20 000 plates/m with pressure only (such as would be in open tubular liquid chromatography), and 480 000 plates/m for electrophoresis with simultaneous counter electroosmotic flow and forward pressure assistance, which validates the simulation data
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban capillary electrophoresis electroosmotic flow pressure-assisted flow separation efficiency
Megjelenés:	Journal of Separation Science. - 41 : 11 (2018), p. 2473-2478. -
További szerzők:	Szigeti Márton (1986-) (környezetmérnök) Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:	OTKA K 116263 OTKA TÉT_15-1-2016-0041 Egyéb BIONANO_GINOP-2.3.2-15-2016-00017 GINOP
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