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1.

001-es BibID:BIBFORM084562
035-os BibID:(Scopus)84961282078 (WOS)000379133500002
Első szerző:Bodnár Judit
Cím:Enzymatic removal of N-glycans by PNGase F coated magnetic microparticles / Bodnar Judit, Szekrenyes Akos, Szigeti Marton, Jarvas Gabor, Krenkova Jana, Foret Frantisek, Guttman Andras
Dátum:2016
ISSN:0173-0835 1522-2683
Megjegyzések:Investigation of protein glycosylation is an important area in biomarker discovery and biopharmaceutical research. Alterations in protein N-glycosylation can be an indication of changes in pathological conditions in the medical field or production parameters of biotherapeutics. Rapid development of these disciplines calls for fast, high-throughput, and reproducible methods to analyze protein N-glycosylation. Currently used methods require either long deglycosylation times or large excess of enzymes. In this paper, we report on the use of PNGase F immobilization onto the surface of magnetic microparticles and their use in rapid and efficient removal of N-glycans from glycoproteins. The use of immobilized PNGase F also allowed reusability of the enzyme-coated beads as the magnetic microparticles can be readily partitioned from the sample by a magnet after each deglycosylation reaction. The efficiency and activity of the PNGase F coated magnetic beads was compared with in-solution enzyme reactions using standard glycoproteins possessing the major N-glycan types of neutral, high mannose, and highly sialylated carbohydrates. The PNGase F coated magnetic beads offered comparable deglycosylation level to the conventional in-solution based method in 10-min reaction times for the model glycoproteins of immunoglobulin G (mostly neutral carbohydrates), ribonuclease B (high mannose type sugars), and fetuin (highly sialylated oligosaccharides) with the special features of easy removal of the enzyme from the reaction mixture and reusability.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Deglycosylation
Enzyme immobilization
Magnetic microparticles
PNGase F
Megjelenés:Electrophoresis. - 37 : 10 (2016), p. 1264-1269. -
További szerzők:Szekrényes Ákos (1983-) (vegyészmérnök) Szigeti Márton (1986-) (környezetmérnök) Járvás Gábor (1982-) (vegyészmérnök) Krenkova, Jana Foret, František Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:K116263
NKFIH
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DOI
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2.

001-es BibID:BIBFORM085475
Első szerző:Járvás Gábor (vegyészmérnök)
Cím:Characterization of a Porous Nano-electrospray Capillary Emitter at Ultra-low Flow Rates / Jarvas Gabor, Fonslow Bryan, Yates John R., Foret Frantisek, Guttman Andras
Dátum:2017
ISSN:0021-9665
Megjegyzések:Biopharmaceuticals, especially therapeutic monoclonal antibodies, have emerged as a very promising new generation of protein-based drugs. However, their comprehensive analysis continues to pose new challenges for the bioanalytical field. Hyphenation of capillary electrophoresis with electrospray ionization (CE-MS) is a promising technique to address these challenges. One of the main advantages of CE-MS is the ability to produce stable electrospray at ultra-low flow rates (5?20 nl/min range). In this short communication we report on the characterization of a porous nanoelectrospray capillary emitter focusing on the effects of ultra-low flow rate on ionization efficiency, ion suppression and detection sensitivity. Ion suppression effect of a poorly-ionizable sugar in the presence of an easily-ionized peptide was reduced by almost 2-fold. Intact therapeutic antibody infusion analysis demonstrated that MS detection sensitivity increased by an order of magnitude with the decrease of flow rate from 250 nL/min to 20 nL/min using the nano-electrospray capillary emitter.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal Of Chromatographic Science. - 55 : 1 (2017), p. 47-51. -
További szerzők:Fonslow, Bryan Yates, John R. Foret, František Guttman András (1954-) (vegyészmérnök)
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DOI
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3.

001-es BibID:BIBFORM083125
035-os BibID:(WoS)000508387300005 (Scopus)85074228284
Első szerző:Járvás Gábor (vegyészmérnök)
Cím:Practical sample pretreatment techniques coupled with capillary electrophoresis for real samples in complex matrices / Jarvas Gabor, Guttman Andras, Miekus Natalia, Baczek Tomasz, Jeong Sunkyung, Chung Doo Soo, Pätoprsty Vladimir, Masár Marián, Hutta Milan, Datinská Vladimira, Foret Frantisek
Dátum:2020
ISSN:0165-9936
Megjegyzések:By coupling a sample pretreatment technique of sample clean up and enrichment power with capillary electrophoresis (CE) of high-performance separation, the task of analyzing trace analytes in a complex matrix such as a biological sample can be carried out successfully with ease. This review aims for providing an overview of strategies to couple sample pretreatment techniques with capillary and related microscale (e.g., microchip) electrophoresis, practically adoptable in an automatic manner, without requiring serious modification of existing instruments to install sophisticated interfaces. In-line sample pretreatment techniques based on liquid phase microextraction performed before sample injection and on-line sample preconcentration techniques performed during or after sample injection are discussed with emphasis on the applicability to samples of high conductivity, commonly encountered for biological samples. An overview of the recent developments in microfluidic immobilized enzymatic microreactors which fit excellently to microchip CE is also given.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Trac-Trends In Analytical Chemistry. - 122 (2020), p. 2-9. -
További szerzők:Guttman András (1954-) (vegyészmérnök) Miȩkus, Natalia Bączek, Tomasz Jeong, Sunkyung Chung, Doo Soo Pätoprstý, Vladimir Masár Marián Hutta, Milan Datinská, Vladimira Foret, František
Pályázati támogatás:Bionano_GINOP-2.3.2-15-2016-00017
GINOP
K116263
NKFIH
NN127062
NKFIH
2018-2.1.17-TET-KR-2018-00010
NKFIH
ÚNKP-19-4
Egyéb
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4.

001-es BibID:BIBFORM050938
Első szerző:Krenkova, Jana
Cím:Oriented immobilization of peptide-N-glycosidase F on a monolithic support for glycosylation analysis / Jana Krenkova, Akos Szekrenyes, Zsolt Keresztessy, Frantisek Foret, Andras Guttman
Dátum:2013
ISSN:0021-9673
Megjegyzések:In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid, reproducible and efficient release of the N-linked carbohydrate moieties from glycoproteins. The glutathione-S-transferase-fusion PNGase F (PNGase F-GST) was expressed in Escherichia coli using regular vector technology. The monolithic pore surface was functionalized with glutathione via a succinimidyl-6-(iodoacetyl-amino)-hexanoate linker and the specific affinity of GST toward glutathione was utilized for the oriented coupling. This novel immobilization procedure was compared with reductive amination technique commonly used for non-oriented enzyme immobilization via primary amine functionalities. Both coupling approaches were compared using enzymatic treatment of several glycoproteins, such as ribonuclease B, fetuin and immunoglobulin G followed by MALDI/MS and CE-LIF analysis of the released glycans. Orientedly immobilized PNGase F via GST-glutathione coupling showed significantly higher activity, remained stable for several months, and allowed rapid release of various types of glycans (high-mannose, core fucosylated, sialylated, etc.) from glycoproteins. Complete protein deglycosylation was obtained as fast as in several seconds when using flow-through immobilized microreactors.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Enzyme microreactor
Oriented immobilization
Monolith
PNGase F
Deglycosylation
Megjelenés:Journal of Chromatography A. - 1322 (2013), p. 54-61. -
További szerzők:Szekrényes Ákos (1983-) (vegyészmérnök) Keresztessy Zsolt Foret, František Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:K-81839
OTKA
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Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM050953
Első szerző:Smejkal, Petr
Cím:Chip-based CE for rapid separation of 8-aminopyrene-1,3,6-trisulfonic acid (APTS) derivatized glycans / Petr Smejkal, Ákos Szekrényes, Markéta Ryvolová, František Foret, András Guttman, Fritz Bek, Mirek Macka
Dátum:2010
ISSN:0173-0835
Megjegyzések:Fluorescently labeled carbohydrates released from glycoproteins were separated using a commercially available microfluidic chip electrophoresis system. While the instrumentation was primarily designed for DNA analysis it was found that the application base can be easily expanded using the development software provided by the manufacturer. The carbohydrates were released by enzymatic digestion (PNGase F) from glycoproteins present in human plasma after boronic acid ? lectin affinity enrichment. After fluorescent labeling with 8-aminopyrene-1,3,6-trisulfonic acid the carbohydrates were separated based on capillary gel electrophoresis mechanism and detected by a fluorescence detector using a blue (470?nm) LED. The separation was completed in 40?s in a microfluidic channel of 14?mm length. Glucose ladder carbohydrate oligomers differing by one glucose unit were baseline separated up to a 20-mer with the main limitation being the detection sensitivity. As expected, the observed resolution in these experiments did not approach that of standard CE with 20 times longer separation distance; however, the chip-based analysis excelled in the speed of the separation. Similar electrophoretic profiles of glycans released from plasma glycoproteins were obtained using a standard CE equipment with 35?cm separation length and microfluidic chips with a separation distance of only 14?mm.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
APTS
Bioanalyzer
Chip-based analysis
Glycans
PSA
Megjelenés:Electrophoresis. - 31 : 22 (2010), p. 3783-3786. -
További szerzők:Szekrényes Ákos (1983-) (vegyészmérnök) Ryvolová, Markéta Foret, František Guttman András (1954-) (vegyészmérnök) Bek, Fritz Macka, Mirek
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Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM058348
Első szerző:Szekrényes Ákos (vegyészmérnök)
Cím:Sample preparation for N-glycosylation analysis of therapeutic monoclonal antibodies by electrophoresis / Ákos Szekrényes, Jan Partyka, Csaba Varadi, Jana Krenkova, Frantisek Foret, András Guttman
Dátum:2015
Megjegyzések:There are a considerable number of biopharmaceuticals that have been approved for clinical use in the past decade. Over half of these new generation drugs are glycoproteins, such as monoclonal antibodies or other recombinant glycoproteins, which are mostly produced in mammalian cell lines. The linked carbohydrate moieties affect not only their physicochemical properties and thermal stability but also crucial features like receptor-binding activity, circulating half-life, as well as immunogenicity. The structural diversity of these attached glycans can be manifested in altered monosaccharide composition and linkages/positions among the monosaccharide building blocks. In addition, as more and more biosimilar products hit the market, understanding the effects of their glycosylation modification has become a recent target in efficacy and safety issues. To ensure consistent quality of these products, glycosylation profiles have to be monitored and controlled in all steps of the manufacturing process, i.e., from clone selection to lot release. In this paper, we describe some of the recently introduced and commonly used sample preparation techniques for capillary electrophoresis (CE)-based profiling and structural elucidation of N-glycans. The presented protocols include protein A affinity partitioning of monoclonal antibodies (mAbs), enzymatic release of the N-linked glycans, labeling of the liberated carbohydrates, reaction mixture purification techniques to remove the excess labeling reagent, and high-resolution and rapid capillary electrophoresis-laser-induced fluorescence (CE-LIF)-based profiling of the labeled and purified N-glycans.
ISBN:978-1-4939-2353-3
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
N-glycosylation analysis
Megjelenés:Microchip Capillary Electrophoresis Protocols : Methods in Molecular Biology / Ann Van Schepdael. - p. 183-195. -
További szerzők:Partyka, Jan Váradi Csaba (1988-) (molekuláris biológus) Krenkova, Jana Foret, František Guttman András (1954-) (vegyészmérnök)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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