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001-es BibID:BIBFORM092907
035-os BibID:(WoS)000584418100065 (Scopus)85096830637
Első szerző:Drouin, Nicolas
Cím:Capillary Electrophoresis-Mass Spectrometry at Trial by Metabo-Ring: Effective Electrophoretic Mobility for Reproducible and Robust Compound Annotation / Nicolas Drouin, Marlien van Mever, Wei Zhang, Elena Tobolkina, Sabrina Ferre, Anne-Catherine Servais, Marie-Jia Gou, Laurent Nyssen, Marianne Fillet, Guinevere S. M. Lageveen-Kammeijer, Jan Nouta, Andrew J. Chetwynd, Iseult Lynch, James A. Thorn, Jens Meixner, Christopher Lößner, Myriam Taverna, Sylvie Liu, N. Thuy Tran, Yannis Francois, Antony Lechner, Reine Nehmé, Ghassan AlHamoui Dit Banni, Rouba Nasreddine, Cyril Colas, Herbert H. Lindner, Klaus Faserl, Christian Neusüß, Manuel Nelke, Stefan Lämmerer, Catherine Perrin, Claudia Bich-Muracciole, Coral Barbas, Ángeles López Gonzálvez, Andras Guttman, Marton Szigeti, Philip Britz-McKibbin, Zachary Kroezen, Meera Shanmuganathan, Peter Nemes, Erika P. Portero, Thomas Hankemeier, Santiago Codesido, Víctor González-Ruiz, Serge Rudaz, Rawi Ramautar
Dátum:2020
ISSN:0003-2700
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Analytical Chemistry. - 92 : 20 (2020), p. 14103-14112. -
További szerzők:van Mever, Marlien Zhang, Wei Tobolkina, Elena Ferre, Sabrina Servais, Anne-Catherine Gou, Marie-Jia Nyssen, Laurent Fillet, Marianne Lageveen-Kammeijer, Guinevere S. M. Nouta, Jan Chetwynd, Andrew J. Lynch, Iseult Thorn, James A. Meixner, Jens Lößner, Christopher Taverna, Myriam Liu, Sylvie Tran, N. Thuy Francois, Yannis Lechner, Antony Nehmé, Reine AlHamoui Dit Banni, Ghassan Nasreddine, Rouba Colas, Cyril Lindner, Herbert H. Faserl, Klaus Neusüß, Christian Nelke, Manuel Lämmerer, Stefan Perrin, Catherine Bich-Muracciole, Claudia Barbas, Coral Gonzálvez, Ángeles López Guttman András (1954-) (vegyészmérnök) Szigeti Márton (1986-) (környezetmérnök) Britz-McKibbin, Philip Kroezen, Zachary Shanmuganathan, Meera Nemes Péter Portero, Erika P. Hankemeier, Thomas Codesido, Santiago González-Ruiz, Víctor Rudaz, Serge Ramautar, Rawi
Pályázati támogatás:NKFIH NN127062
egyéb
2018-2.1.17-TÉT-KR-2018-00010
egyéb
BIONANO_GINOP-2.3.2-15-2016-00017
GINOP
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2.

001-es BibID:BIBFORM099698
035-os BibID:(cikkazonosító)462593
Első szerző:Liénard--Mayor, Théo
Cím:High sensitivity capillary electrophoresis with fluorescent detection for glycan mapping / Liénard--Mayor Théo, Yang Bin, Tran Nguyet Thuy, Bruneel Arnaud, Guttman Andras, Taverna Myriam, Mai Thanh Duc
Dátum:2021
ISSN:0021-9673
Megjegyzések:We present in this study a novel strategy to drastically improve the detection sensitivity and peak capac- ity for capillary electrophoresis with laser induced fluorescent detection (CE-LIF) of glucose oligomers and released glycans. This is based on a new approach exploiting a polymer-free background electrolyte (BGE) for CE-LIF of glycans. The best performance in terms of sample stacking and suppression of electroos- motic flow (EOF) was found for a BGE composed of triethanolamine/citric acid and triethanolamine/acetic acid at elevated ionic strengths (IS up to 200 mM). Compared to the conventional protocols for CE- LIF of glucose-oligosaccharides and released glycans, our polymer-free strategy offered up to 5-fold im- provement of detection sensitivity and visualization of higher degree of polymerization (DP) of glucose oligomers (18 vs 15). To further improve the detection sensitivity, a new electrokinetic preconcentration strategy via large volume sample stacking with electroosmotic modulation without having recourse to neutrally coated capillaries is proposed, offering a 200-fold signal enhancement. This approach is based on variation of the buffer's IS, rather than pH adjustment as in conventional methods, for EOF modula- tion or quasi-total reduction. This strategy allows selecting with high flexibility the best pH conditions to perform efficient preconcentration and separation. The new approach was demonstrated to be applicable for the analysis of N-linked oligosaccharides released from a model glycoprotein (Human Immunoglobu- lin G) and applied to map N-glycans from human serum for congenital disorders of glycosylation (CDG) diagnosis.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Chromatography A. - 1657 (2021), p. 1-9. -
További szerzők:Yang, Bin Tran, N. Thuy Bruneel, Arnaud Guttman András (1954-) (vegyészmérnök) Taverna, Myriam Mai, Thanh Duc
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3.

001-es BibID:BIBFORM092909
035-os BibID:(WoS)000579368600001 (Scopus)85089666849
Első szerző:Shao, Huikai
Cím:On-line enrichment of N-glycans by immobilized metal-affinity monolith for capillary electrophoresis analysis / Huikai Shao, Balazs Reider, Gabor Jarvas, Andras Guttman, Zhengjin Jiang, N. Thuy Tran, Myriam Taverna
Dátum:2020
ISSN:0003-2670
Megjegyzések:A novel N-glycan enrichment strategy is presented using unexpected but strong interactions between the sulfonate groups brought by the fluorescent dye of glycans and the Zr4+ modified poly(ethylene glycol methacrylate phosphate (EGMP)-co-acrylamide (AM)-co-bis-acrylamide (BAA)) monolith. The poly (EGMP-co-AM-co-BAA) monolith was synthesized via ultraviolet (UV) irradiation and then functionalized with Zr4+. The obtained monolith was characterized with scanning electron microscopy and mercury intrusion porosimetry. Large through-pores and a continuous skeleton with high permeability were observed. The N-glycans were labeled with the 1-aminopyrene-3, 6, 8-trisulfonic acid (APTS) and enriched by the Zr4+ modified monolith through IMAC interaction. This enrichment step was then coupled off-line to capillary electrophoresis (CE) separation with laser induced fluorescence (LIF) detection. Successful preconcentration of the APTS labeled maltooligosaccharide ladder was achieved under optimized conditions. Enrichment factors obtained for the maltooligosaccharides ranged from 9 to 24 with RSDs from 2.0% to 9.2% (n = 3). Moreover, very good repeatabilities (<6.7%) were obtained for glucose oligomers (4?15 glucose units) corresponding to sizes expected for N-glycans, demonstrating the great potential of this Zr4+ modified monolith to enrich APTS labeled glycans from N-glycoproteins. The proposed method was then successfully applied for the enrichment of N-glycans released from Ribonuclease B, in which case all five expected oligomannose glycans (Man 5 to Man 9) were successfully enriched. Thanks to the advantage of the method to enrich selectively APTS-glycans compared to the commercial SPE columns composed of HILIC or PGC materials, the first proof of concept of on-line enrichment coupled to CE-LIF separation was demonstrated for maltooligosaccharides as well.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Analytica Chimica Acta. - 1134 (2020), p. 1-9. -
További szerzők:Reider Balázs Járvás Gábor (1982-) (vegyészmérnök) Guttman András (1954-) (vegyészmérnök) Jiang, Zhengjin Tran, N. Thuy Taverna, Myriam
Pályázati támogatás:BIONANO_GINOP-2.3.2-15-2016-00017
GINOP
ÚNKP-19-4
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