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001-es BibID:	BIBFORM099697
035-os BibID:	(cikkazonosító)6399 (WoS)000718435900001 (Scopus)85117579488
Első szerző:	Török Rebeka
Cím:	N-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis : a Preliminary Study / Torok Rebeka, Horompoly Klaudia, Szigeti Marton, Guttman Andras, Vitai Marta, Koranyi Laszlo, Jarvas Gabor
Dátum:	2021
ISSN:	1420-3049
Megjegyzések:	Currently, diagnosing type 2 diabetes (T2D) is a great challenge. Thus, there is a need to find rapid, simple, and reliable analytical methods that can detect the disease at an early stage. The aim of this work was to shed light on the importance of sample collection options, sample preparation conditions, and the applied capillary electrophoresis bioanalytical technique, for a highresolution determination of the N-glycan profile in human blood samples of patients with type 2 diabetes (T2D). To achieve the profile information of these complex oligosaccharides, linked by asparagine to hIgG in the blood, the glycoproteins of the samples needed to be cleaved, labelled, and purified with sufficient yield and selectivity. The resulting samples were analyzed by capillary electrophoresis, with laser-induced fluorescence detection. After separation parameter optimization, the capillary electrophoresis technique was implemented for efficient N-glycan profiling of whole blood samples from the diabetic patients. Our results revealed that there were subtle differences between the N-glycan profiles of the diabetic and control samples; in particular, two N-glycan structures were identified as potential glycobiomarkers that could reveal significant changes between the untreated/treated type 2 diabetic and control samples. By analyzing the resulting oligosaccharide profiles, clinically relevant information was obtained, revealing the differences between the untreated and HMG-CoA reductase-inhibitor-treated diabetic patients on changes in the N-glycan profile in the blood. In addition, the information from specific IgG N-glycosylation profiles in T2D could shed light on underlying inflammatory pathophysiological processes and lead to drug targets.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk N-glycan type 2 diabetes capillary electrophoresis biomarker blood collecting tubes
Megjelenés:	Molecules. - 26 : 21 (2021), p. 1-11. -
További szerzők:	Horompoly Klaudia Szigeti Márton (1986-) (környezetmérnök) Guttman András (1954-) (vegyészmérnök) Vitai Márta (1961-) (okleveles vegyész) Korányi László Járvás Gábor (1982-) (vegyészmérnök)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM092960
Első szerző:	Török Rebeka
Cím:	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples / Rebeka Torok, Anna Farkas, András Guttman, Gabor Jarvas
Dátum:	2020
ISSN:	1566-5240
Megjegyzések:	The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research. However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies. In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection. Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance. Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results. Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures. Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Current Molecular Medicine. - 20 : 10 (2020), p. 840-846. -
További szerzők:	Farkas Anna (1988-) (molekuláris biológus) Guttman András (1954-) (vegyészmérnök) Járvás Gábor (1982-) (vegyészmérnök)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: