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001-es BibID:	BIBFORM114595
035-os BibID:	(scopus)85169450797 (WOS)001058799600001
Első szerző:	Kormos József (fizikus)
Cím:	HLA DQ protein changes the cell surface distribution pattern of HLA proteins as monitored by Förster resonance energy transfer and high-resolution electron microscopy / Kormos József, Veres Adrienn J., Imre László, Mátyus László, Benkő Szilvia, Szöllősi János, Jenei Attila
Dátum:	2023
ISSN:	1552-4922 1552-4930
Megjegyzések:	Peptide presentation by MHC class I and MHC class II molecules plays important roles in the regulation of the immune response. One factor in these displays is the density of antigen, which must exceed a critical threshold for the effective activation of T cells. Nonrandom distribution of MHC class I and class II has already been detected at the nanometer level and at higher hierarchical levels. It is not clear how the absence and reappearance of some protein molecules can influence the nonrandom distribution. Therefore, we performed experiments on HLA II-deficient bare lymphocyte syndrome (BLS1) cells: we created a stable transfected cell line, tDQ6-BLS-1, and were able to detect the effect of the appearance of HLA-DQ6 molecules on the homo and heteroassociation of different cell surface molecules by comparing Förster resonance energy transfer (FRET) efficiency on transfected cells to that on nontransfected BLS-1 and JY human B-cell lines. Our FRET results show a decrease in homoassociation FRET between HLA I chains in HLA-DQ6-transfected tDQ6-BLS-1 cells compared with the parent BLS-1 cell line and an increase in heteroassociation FRET between HLA I and HLA II (compared with JY cells), suggesting a similar pattern of antigen presentation by the HLA-DQ6 allele. Transmission electron microscopy (TEM) revealed that both HLA class I and class II molecules formed clusters at higher hierarchical levels on the tDQ6-BLS-1 cells, and the de novo synthesized HLA DQ molecules did not intersperse with HLA class I islands. These observations could be important in understanding the fine tuning of the immune response.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk BLS-1 FRET HLA-DQ6 immunogold labeling MHC TEM
Megjelenés:	Cytometry Part A. - 103 : 12 (2023), p. 978-991. -
További szerzők:	Veres Adrienn J. (biofizikus) Imre László (1979-) (biológus) Mátyus László (1956-) (biofizikus) Benkő Szilvia (1973-) (molekuláris biológus) Szöllősi János (1953-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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