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1.
001-es BibID:
BIBFORM052738
035-os BibID:
PMID: 23276530
Első szerző:
Lahav, Judith
Cím:
Factor XIII improves platelet adhesion to fibrinogen by protein disulfide isomerase-mediated activity / Judith Lahav, Ariella Tvito, Zsuzsa Bagoly, Rima Dardik, Aida Inbal
Dátum:
2013
ISSN:
0049-3848
Megjegyzések:
BACKGROUND: Factor XIII (FXIII), a plasma pro-transglutaminase, consists of two A subunits and two B subunits (FXIIIA2B2). Following activation by thrombin, it cross-links fibrin chains at the final step of coagulation. We previously reported that FXIII subunit A (FXIIIA) serves as a protein disulfide isomerase (PDI), and that PDI promotes platelet adhesion and aggregation. OBJECTIVE: This study sought to examine possible mechanistic effect of FXIII on platelet adhesion to fibrinogen; specifically, the role of its PDI activity. METHODS: Ex vivo experiments: Blood platelets derived from five patients with hereditary FXIIIA deficiency before and after treatment with Fibrogammin-P (FXIIIA2B2 concentrate) were washed and incubated on immobilized fibrinogen. Bound platelets were stained and counted by microscopy. In vitro experiments: Platelets derived from patients before treatment and five healthy controls were washed and analyzed for adhesion in the presence or absence of Fibrogammin-P or recombinant FXIII (FXIIIA2 concentrate). RESULTS: In ex vivo experiments, one hour after Fibrogammin-P treatment, mean (±SEM) platelet adhesion to fibrinogen increased by 27±2.32% (p<0.001). In in vitro experiments, treatment with Fibrogammin-P or recombinant FXIII (10IU/mL each) enhanced platelet adhesion to fibrinogen (in patients, by 29.95±6.7% and 29.05±5.3%, respectively; in controls, by 26.06±3.24% and 26.91±4.72, respectively; p<0.04 for all). Iodoacetamide-treated FXIII (I-FXIII), where transglutaminase activity is blocked, showed similar enhanced adhesion as untreated FXIII. By contrast, addition of an antibody that specifically blocks FXIIIA-PDI activity inhibited FXIII-mediated platelet adhesion to fibrinogen by 65%. CONCLUSION: These findings indicate that FXIII-induced enhancement of platelet adhesion is mediated by FXIII-PDI activity.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
PDI, Factor XIII, Transglutaminase, platelet adhesion
Megjelenés:
Thrombosis Research. - 131 : 4 (2013), p. 338-341. -
További szerzők:
Tvito, Ariella
Bagoly Zsuzsa (1978-) (orvos)
Dardik, Rima
Inbal, Aida
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:
2.
001-es BibID:
BIBFORM010446
Első szerző:
Lahav, Judith
Cím:
Coagulation factor XIII serves as protein disulfide isomerase / Lahav, J., Karniel, E., Bagoly, Z., Sheptovitsky, V., Dardik, R., Inbal, A.
Dátum:
2009
ISSN:
0340-6245 (Print)
Megjegyzések:
Tissue transglutaminase was reported to act as protein disulfide isomerase (PDI). We studied whether plasma transglutaminase - coagulation factor XIII (FXIII) - has PDI activity as well. PDI activity was measured by determining the ability to renature reduced-denatured RNase (rdRNase). We found that FXIII can renature rdRNase, with efficiency comparable to commercial PDI. This PDI activity was inhibited by bacitracin. Like tissue transglu-taminase, FXIII-mediated PDI activity is independent of its transglutaminase activity and is located on the A subunit. Surface-associated PDI has been previously shown to catalyse two distinct functions: transnitrosation with subsequent release of intracellular nitric oxide and disulfide bond rearrangement during platelet integrin ligation. Our results imply that FXIII-PDI activity may have a role in platelet function.
Tárgyszavak:
Orvostudományok
Klinikai orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Factor XIII
PDI
Transglutaminase
bacitracin
blood clotting factor 8
integrin
nitric oxide
protein disulfide isomerase
ribonuclease
alpha chain
article
catalysis
disulfide bond
nitrosation
priority journal
protein binding
protein determination
surface property
thrombocyte function
Animals
Antibodies
Bacitracin
Cattle
Enzyme Inhibitors
Factor XIII
Factor XIIIa
Humans
Protein Disulfide-Isomerases
Protein Renaturation
Protein Subunits
Ribonuclease
Pancreatic
Megjelenés:
Thrombosis and Haemostasis. - 101 : 5 (2009), p. 840-844. -
További szerzők:
Karniel, Eli
Bagoly Zsuzsa (1978-) (orvos)
Sheptovitsky, Vera
Dardik, Rima
Inbal, Aida
Internet cím:
elektronikus változat
Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:
Saját polcon:
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