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001-es BibID:	BIBFORM077046
035-os BibID:	(WoS)000485146200010 (Scopus)85070584761
Első szerző:	Deutschmann, Claudia
Cím:	Identification of Chitinase-3-like protein 1 as a novel neutrophil antigenic target in Crohn's disease / Deutschmann Claudia, Sowa Mandy, Murugaiyan Jayaseelan, Roesler Uwe, Röber Nadja, Conrad Karsten, Laass Martin, Bogdanos Dimitrios, Sipeki Nora, Papp Maria, Rödiger Stefan, Roggenbuck Dirk, Schierack Peter
Dátum:	2019
ISSN:	1873-9946
Megjegyzések:	Background and Aims: There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods: Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization - time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn's disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results: The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p < 0.0001, respectively] and are associated with a more complicated progression of CD. Conclusion: CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk inflammatory bowel disease chitinase-3 like protein 1 autoantibody Crohn's disease enzyme-linked immunosorbent assay
Megjelenés:	Journal of Crohns & Colitis. - 13 : 7 (2019), p. 894-904. -
További szerzők:	Sowa, Mandy Murugaiyan, Jayaseelan Roesler, Uwe Röber, Nadja Conrad, Karsten Laass, Martin Bogdanos, Dimitrios P. Sipeki Nóra (1987-) (általános orvos) Papp Mária (1975-) (belgyógyász, gasztroenterológus) Rödiger, Stefan Roggenbuck, Dirk Schierack, Peter
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001-es BibID:	BIBFORM074575
035-os BibID:	(cikkazonosító)1959 (WoS)000442936400001 (Scopus)85068120394
Első szerző:	Sowa, Mandy
Cím:	Mucosal autoimmunity to cell-bound GP2 isoforms is a sensitive marker in PSC and associated with the clinical phenotype / Mandy Sowa, Rafał Kolenda, Daniel Baumgart, Johann Pratschke, Maria Papp, Tamas Tornai, Jaroslaw Suchanski, Dimitrios Bogdanos, Maria Mytilinaiou, Jutta Hammermann, Martin Laass, Karsten Conrad, Christoph Schramm, Andre Franke, Dirk Roggenbuck, Peter Schierack
Dátum:	2018
ISSN:	1664-3224
Megjegyzések:	Introduction: Zymogen granule glycoprotein 2 (GP2) was demonstrated as first autoimmune mucosal target in primary sclerosing cholangitis (PSC) associated with disease severity. Autoantibodies to four GP2 isoforms (aGP21-4) were found in patients with inflammatory bowel diseases but reactivity against specific GP2 epitopes has not been investigated in PSC yet. Hence, the prevalence of aGP21-4 and their association with the PSC phenotype for risk prediction were examined.Methods: GP2 isoforms were stably expressed as glycosylphosphatidylinositol- anchored molecules in the membrane of HEp-2-cells and used as autoantigenic targets in indirect immunofluorescence assay (IFA). aGP21-4 IgA and IgG were detected by IFA in 212 PSC patients of four European university hospitals and 145 controls comprising 95 patients with cystic fibrosis and 50 healthy subjects.Results: Combined aGP21 and aGP24 IgA testing with a sensitivity of 66.0% and a specificity of 97.9% resulted in the best diagnostic performance (Youden index: 0.64) regarding all aGP2 and combinations thereof. aGP24 IgA positivity is significantly associated with the presence of cirrhosis in PSC (p=0.0056). Logistic regression revealed the occurrence of aGP21 IgA (odds ratio [OR] 1.38, 95% confidence interval [CI]: 1.03-1.86) and aGP24 IgA (OR 1.52, 95%CI: 1.07-2.15) along with male gender (OR 0.51, 95%CI: 0.27-0.97) and older age (OR 1.03 95%CI: 1.01-1.05) as significant risks for the concomitant presence of cirrhosis in PSC.Conclusions: Combined aGP21 and aGP24 IgA analysis is preferred to single aGP2 isoform analysis for sensitive PSC autoantibody testing. Positivity for aGP21 and aGP24 IgA is associated with cirrhosis in PSC and could be used for risk stratification.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk zymogen granule glycoprotein 2 primary sclerosing cholangitis cirrhosis cholangiocarcinoma immunoglobulin A
Megjelenés:	Frontiers in Immunology. - 9 (2018), p. 1-10. -
További szerzők:	Kolenda, Rafał Baumgart, Daniel Pratschke, Johann Papp Mária (1975-) (belgyógyász, gasztroenterológus) Tornai Tamás István (1984-) (belgyógyász) Suchanski, Jaroslaw Bogdanos, Dimitrios P. Mytilinaiou, Maria Hammermann, Jutta Laass, Martin Conrad, Karsten Schramm, Christoph Franke, Andre Roggenbuck, Dirk Schierack, Peter
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