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001-es BibID:	BIBFORM010417
Első szerző:	Borbély Attila (kardiológus)
Cím:	Transcriptional and posttranslational modifications of titin : implications for diastole / Borbely, A., van Heerebeek, L., Paulus, W. J.
Dátum:	2009
ISSN:	1524-4571 (Electronic)
Tárgyszavak:	Orvostudományok Klinikai orvostudományok szerkesztőségi anyag Alternative Splicing Animals Cyclic AMP-Dependent Protein Kinases Cyclic GMP-Dependent Protein Kinases Diabetes Complications Diacetyl derivatives Diastole Elasticity Extracellular Matrix Gelsolin Heart Failure, Diastolic Humans Mice Muscle Proteins Myocytes, Cardiac Phosphorylation Protein Isoforms Protein Kinases Protein Processing, Post-Translational Rats Sarcomeres Triiodothyronine Ventricular Remodeling
Megjelenés:	Circulation Research. - 104 : 1 (2009), p. 12-14. -
További szerzők:	Heerebeek, Loek, van Paulus, Walter J.
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001-es BibID:	BIBFORM010415
Első szerző:	Borbély Attila (kardiológus)
Cím:	Hypophosphorylation of the Stiff N2B titin isoform raises cardiomyocyte resting tension in failing human myocardium / Borbely, A., Falcao-Pires, I., van Heerebeek, L., Hamdani, N., Edes, I., Gavina, C., Leite-Moreira, A. F., Bronzwaer, J. G. F., Papp, Z., van der Velden, J., Stienen, G. J. M., Paulus, W. J.
Dátum:	2009
ISSN:	1524-4571 (Electronic)
Megjegyzések:	High diastolic stiffness of failing myocardium results from interstitial fibrosis and elevated resting tension (F(passive)) of cardiomyocytes. A shift in titin isoform expression from N2BA to N2B isoform, lower overall phosphorylation of titin, and a shift in titin phosphorylation from N2B to N2BA isoform can raise F(passive) of cardiomyocytes. In left ventricular biopsies of heart failure (HF) patients, aortic stenosis (AS) patients, and controls (CON), we therefore related F(passive) of isolated cardiomyocytes to expression of titin isoforms and to phosphorylation of titin and titin isoforms. Biopsies were procured by transvascular technique (44 HF, 3 CON), perioperatively (25 AS, 4 CON), or from explanted hearts (4 HF, 8 CON). None had coronary artery disease. Isolated, permeabilized cardiomyocytes were stretched to 2.2-microm sarcomere length to measure F(passive). Expression and phosphorylation of titin isoforms were analyzed using gel electrophoresis with ProQ Diamond and SYPRO Ruby stains and reported as ratio of titin (N2BA/N2B) or of phosphorylated titin (P-N2BA/P-N2B) isoforms. F(passive) was higher in HF (6.1+/-0.4 kN/m(2)) than in CON (2.3+/-0.3 kN/m(2); P<0.01) or in AS (2.2+/-0.2 kN/m(2); P<0.001). Titin isoform expression differed between HF (N2BA/N2B=0.73+/-0.06) and CON (N2BA/N2B=0.39+/-0.05; P<0.001) and was comparable in HF and AS (N2BA/N2B=0.59+/-0.06). Overall titin phosphorylation was also comparable in HF and AS, but relative phosphorylation of the stiff N2B titin isoform was significantly lower in HF (P-N2BA/P-N2B=0.77+/-0.05) than in AS (P-N2BA/P-N2B=0.54+/-0.05; P<0.01). Relative hypophosphorylation of the stiff N2B titin isoform is a novel mechanism responsible for raised F(passive) of human HF cardiomyocytes.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Aged Biopsy Elasticity Female Heart Failure Humans Male Middle Aged Muscle Proteins Myocardium Myocytes, Cardiac Phosphorylation Protein Isoforms Protein Kinases Sarcomeres
Megjelenés:	Circulation Research. - 104 : 6 (2009), p. 780-786. -
További szerzők:	Falcao-Pires, Ines Heerebeek, Loek, van Hamdani, Nazha Édes István (1952-) (kardiológus) Gavina, Cristina Leite-Moreira, Adelino F. Bronzwaer, Jean G. F. Papp Zoltán (1965-) (kardiológus, élettanász) Velden, Jolanda, van der Stienen, Ger J. M. Paulus, Walter J.
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