CCL

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1.

001-es BibID:BIBFORM054868
Első szerző:Bedekovics Judit (orvos)
Cím:Image analysis of platelet derived growth factor receptor-beta (PDGFRβ) expression to determine the grade and dynamics of myelofibrosis in bone marrow biopsy samples / Judit Bedekovics, Szilvia Szeghalmy, Lívia Beke, Attila Fazekas, Gábor Méhes
Dátum:2014
ISSN:1552-4949
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Hálózatok modellezése és analízise
bone marrow
myelofibrosis
PDGFRβ
Megjelenés:Cytometry. Part B, Clinical Cytometry. - 86 : 5 (2014), p. 319-328. -
További szerzők:Szeghalmy Szilvia (1984-) (programtervező matematikus) Beke Lívia Fazekas Attila (1968-) (matematikus, informatikus) Méhes Gábor (1966-) (patológus)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0045
TÁMOP
Patológia Kutatócsoport
TÁMOP-4.2.2.C-11/1/KONV-2012-0001
TÁMOP
Hálózatok modellezése és hatékonyságvizsgálatai
Internet cím:Szerző által megadott URL
DOI
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2.

001-es BibID:BIBFORM019452
Első szerző:Bollmann, Reinhard
Cím:Determination of ploidy and steroid receptor status in breast cancer by laser scanning cytometry / Reinhard Bollmann, Robert Torka, Jörg Schmitz, Magdolna Bollmann, Gábor Méhes
Dátum:2002
ISSN:0196-4763
Megjegyzések:BACKGROUND: Measurements on DNA content and steroid receptor status in breast cancer are of great clinical interest. Objective determination of estrogen and progesterone receptor expression should help to define the lowest levels of positivity still responding to adjuvant antihormonal therapy. For this purpose, a simple protocol for laser scanning cytometry is presented. METHODS: Analysis of 54 routine breast cancer samples was performed by laser scanning cytometry (LSC). To obtain single cell preparations from fresh tumor tissue, slides were prepared using the Cervisoft cytological device. Exact determination of tumor cell DNA content was done by referring to the CD45-positive tissue leukocyte fraction as the internal diploid reference cell population. Steroid receptor-expressing cells were detected by indirect immunolabeling. RESULTS: Indirect immunofluorescence allowed the best quantification of both the estrogen and progesterone receptor-expressing cell fractions by LSC. The number of receptor-expressing cells could be given in percentage. For comparison, the 10% cutoff value was used to determine receptor positivity. CONCLUSION: LSC enabled a simple, reliable, and inexpensive determination of DNA index and steroid receptor expression in breast cancer specimens by objective criteria
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Cytometry. - 50 : 4 (2002), p. 210-215. -
További szerzők:Torka, Robert Schmitz, Jörg Bollmann, Magdolna Méhes Gábor (1966-) (patológus)
Internet cím:DOI
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3.

001-es BibID:BIBFORM019439
Első szerző:Kajtár Béla (patológus)
Cím:Automated fluorescent in situ hybridization (FISH) analysis of t(9;22)(q34;q11) in interphase nuclei / Béla Kajtár, Gábor Méhes, Thomas Lörch, Linda Deák, Marika Kneifné, Donát Alpár, László Pajor
Dátum:2006
Megjegyzések:BACKGROUND: For chronic myeloid leukemia, the FISH detection of t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes is an alternative method to bone marrow karyotyping for monitoring treatment. With automation, several drawbacks of manual analysis may be circumvented. In this article, the capabilities of a commercially available automated image acquisition and analysis system were determined by detecting t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes. METHODS: Three peripheral blood samples of normal adults, 21 samples of CML patients, and one sample of a t(9;22)(q34;q11) positive cell-line were used. RESULTS: Single nuclei with correctly detected signals amounted to 99.6% of nuclei analyzed after exclusion of overlapping nuclei and nuclei with incorrect signal detection. A cut-off value of 0.84 mum was defined to discriminate between translocation positive and negative nuclei based on the shortest distance between signals. Using this value, the false positive rate of the automated analysis for negative samples was 7.0%, whereas that of the manual analysis was 5.8%. Automated and manual results showed strong correlation (R(2) = 0.985), the mean difference of results was only 3.7%. CONCLUSIONS: A reliable and objective automated analysis of large numbers of cells is possible, avoiding interobserver variability and producing statistically more accurate results than manual evaluation.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cytometry. Part A. - 69 : 6 (2006), p. 506-514. -
További szerzők:Méhes Gábor (1966-) (patológus) Lörch, Thomas Deák Linda Kneif Mária Alpár Donát Pajor László (patológus)
Internet cím:DOI
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4.

001-es BibID:BIBFORM019459
Első szerző:Méhes Gábor (patológus)
Cím:Quantitative analysis of disseminated tumor cells in the bone marrow by automated fluorescence image analysis / Gábor Méhes, Thomas Lörch, Peter F. Ambros
Dátum:2000
ISSN:0196-4763
Megjegyzések:Accurate quantification of disseminated tumor cells in hematological samples is of fundamental importance in clinical oncology. However, even highly standardized protocols allow only a rough estimation of the total analyzed cell number, as sample processing may have adverse effects on the number of cells available for analysis. The fluorescence-based microscopic scanning system (MetaCyte) detects, counts, captures, and relocates immunolabeled tumor cells in hematopoietic samples. We report on a cell-counting approach that has been implemented into the scanning system to precisely quantify the number of cells per slide. The cell-counting function, which was designed to determine the number of all nucleated (DAPI-stained) cells on the slide, allows an accurate counting of the tumor cells and the total number of cells analyzed in the given microscopic sample. The reliability of the cell-counting approach was demonstrated by the analysis of DAPI-stained images with 18-1,363 nucleated cells. A good correlation (r(2) = 0.965) between the manually and automatically gained results was observed. The counting accuracy could even be optimized after implementing a correction factor. To prove or disprove an interslide variation, routine bone marrow cytospin preparations from neuroblastoma patients were immunostained for GD2/FITC and counterstained with DAPI. Automatic cell counting of cytospin preparations from the same patients showed significant differences in the total cell number (up to 67% cell loss during preparation, with a maximum interslide difference of 4.7 x 10(5) mononuclear cells). We conclude that determination of the tumor cell content in hematopoietic samples is only reliable when it is performed together with accurate cell counting.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Cytometry. - 42 : 6 (2000), p. 357-362. -
További szerzők:Lörch, Thomas Ambros, Peter F.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM019462
Első szerző:Pajor László (patológus)
Cím:Combined metaphase, interphase cytogenetic, and flow cytometric analysis of DNA content of pediatric acute lymphoblastic leukemia / L. Pajor, K. Szuhai, G. Méhes, G. Kosztolányi, P. Jaksó, G. Lendvai, I. Szanyi, P. Kajtár
Dátum:1998
ISSN:0196-4763
Megjegyzések:Eleven pediatric acute lymphoid leukemia patients were investigated for chromosomal aneuploidy by interphase cytogenetics using chromosome specific (peri)centromeric probes for all the somatic and sex chromosomes. Results were compared with metaphase cytogenetic and flow cytometric derived DNA aneuploidy data. Experiments performed on normal human cells using chromosome specific (peri)centromeric probes indicated that disomy could be recognized in a range of 89.1+/-2.7% (12.9)-96.8+/-0.2% (0.9) for the somatic chromosomes and in 98.1+/-0.4% (1.3) for the sex chromosomes. Using the cutoff level of the mean false monosomy and trisomy in the control cells +2 S.D., chromosome loss or gain for the somatic chromosomes could be revealed beyond a clonal ratio of 3.6-13.2% and 1.1-6.8%, respectively. The same value for the sex chromosomes was 3.5% and 0%, respectively. In 5 of 11 patients the leukemic cells proved to be diploid with all three methods at both gross DNA and chromosome levels. Interphase cytogenetics revealed chromosome loss or gain in all of the remaining six patients, however, the metaphase analysis indicated numerical aberration in only two patients. In one of them only the increased chromosome number could have been detected without identifying the chromosomes involved and in the other one the two methods indicated trisomy for a different chromosome. Flow cytometric data showed aneuploidy in three of the six aneuploid leukemia patients. The results suggest that interphase cytogenetics might be more accurate compared with flow cytometry and metaphase analysis to reveal aneuploidy.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cytometry. - 34 : 2 (1998), p. 87-94. -
További szerzők:Szuhai Károly Méhes Gábor (1966-) (patológus) Kosztolányi György Jáksó Pál Lendvai Gábor Szanyi István Kajtár Pál
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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