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001-es BibID:	BIBFORM019439
Első szerző:	Kajtár Béla (patológus)
Cím:	Automated fluorescent in situ hybridization (FISH) analysis of t(9;22)(q34;q11) in interphase nuclei / Béla Kajtár, Gábor Méhes, Thomas Lörch, Linda Deák, Marika Kneifné, Donát Alpár, László Pajor
Dátum:	2006
Megjegyzések:	BACKGROUND: For chronic myeloid leukemia, the FISH detection of t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes is an alternative method to bone marrow karyotyping for monitoring treatment. With automation, several drawbacks of manual analysis may be circumvented. In this article, the capabilities of a commercially available automated image acquisition and analysis system were determined by detecting t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes. METHODS: Three peripheral blood samples of normal adults, 21 samples of CML patients, and one sample of a t(9;22)(q34;q11) positive cell-line were used. RESULTS: Single nuclei with correctly detected signals amounted to 99.6% of nuclei analyzed after exclusion of overlapping nuclei and nuclei with incorrect signal detection. A cut-off value of 0.84 mum was defined to discriminate between translocation positive and negative nuclei based on the shortest distance between signals. Using this value, the false positive rate of the automated analysis for negative samples was 7.0%, whereas that of the manual analysis was 5.8%. Automated and manual results showed strong correlation (R(2) = 0.985), the mean difference of results was only 3.7%. CONCLUSIONS: A reliable and objective automated analysis of large numbers of cells is possible, avoiding interobserver variability and producing statistically more accurate results than manual evaluation.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Cytometry. Part A. - 69 : 6 (2006), p. 506-514. -
További szerzők:	Méhes Gábor (1966-) (patológus) Lörch, Thomas Deák Linda Kneif Mária Alpár Donát Pajor László (patológus)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM113529
035-os BibID:	(Scopus)85161580863 (WOS)001002920800001
Első szerző:	Nagy Ákos
Cím:	Parallel testing of liquid biopsy (ctDNA) and tissue biopsy samples reveals a higher frequency of mutations in follicular lymphoma / Nagy Ákos, Bátai Bence, Kiss Laura, Gróf Stefánia, Király Péter Attila, Jóna Ádám, Demeter Judit, Sánta Hermina, Bátai Árpád, Pettendi Piroska, Szendrei Tamás, Plander Márk, Körösmezey Gábor, Alizadeh Hussain, Kajtár Béla, Méhes Gábor, Krenács László, Timár Botond, Csomor Judit, Tóth Erika, Schneider Tamás, Mikala Gábor, Matolcsy András, Alpár Donát, Masszi András, Bödör Csaba
Dátum:	2023
ISSN:	0954-6820
Megjegyzések:	Background: Recent genomic studies revealed enhancer of zeste homolog 2 (EZH2) gain-of-function mutations, representing novel therapeutic targets in follicular lymphoma (FL) in around one quarter of patients. However, these analyses relied on single-site tissue biopsies and did not investigate the spatial heterogeneity and temporal dynamics of these alterations. Objectives: We aimed to perform a systematic analysis of EZH2 mutations using paired tissue (tumor biopsies [TB]) and liquid biopsies (LB) collected prior to treatment within the framework of a nationwide multicentric study. Methods: Pretreatment LB and TB samples were collected from 123 patients. Among these, 114 had paired TB and LB, with 39 patients characterized with paired diagnostic and relapse samples available. The EZH2 mutation status and allele burden were assessed using an in-house-designed, highly sensitive multiplex droplet digital PCR assay. Results: EZH2 mutation frequency was found to be 41.5% in the entire cohort. In patients with paired TB and LB samples, EZH2 mutations were identified in 37.8% of the patients with mutations exclusively found in 5.3% and 7.9% of TB and LB samples, respectively. EZH2 mutation status switch was documented in 35.9% of the patients with paired diagnostic and relapse samples. We also found that EZH2 wild-type clones may infiltrate the bone marrow more frequently compared to the EZH2 mutant ones. Conclusion: The in-depth spatio-temporal analysis identified EZH2 mutations in a considerably higher proportion of patients than previously reported. This expands the subset of FL patients who most likely would benefit from EZH2 inhibitor therapy.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk ctDNA ddPCR EZH2 follicular lymphoma liquid biopsy tazemetostat
Megjelenés:	Journal Of Internal Medicine. - 294 : 3 (2023), p. 295-313. -
További szerzők:	Bátai Bence Kiss Laura Gróf Stefánia Király Péter Attila Jóna Ádám (1985-) (orvos) Demeter Judit Sánta Hermina Bátai Árpád Pettendi Piroska Szendrei Tamás Plander Márk Körösmezey Gábor Alizadeh, Hussain Kajtár Béla (1977-) (patológus) Méhes Gábor (1966-) (patológus) Krenács László Timár Botond Csomor Judit Tóth Erika Schneider Tamás (onkológus) Mikala Gábor Matolcsy András Alpár Donát Masszi András Bödör Csaba
Pályázati támogatás:	NKFIH KDP-1022882 Egyéb EFOP-3.6.3-VEKOP-16-2017-00009 EFOP ÚNKP-21-3-II-SE24 Egyéb ÚNKP-22-5-SE-7 Egyéb K21-137948 Egyéb FK20_134253 Egyéb TKP2021-EGA-24 Egyéb TKP2021-NVA-15 Egyéb
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