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1.

001-es BibID:BIBFORM019673
Első szerző:Ambros, Peter F.
Cím:Erkennung, Quantifizierung und Charakterisierung von disseminierten Tumorzellen = [Detection, quantification and characterization of disseminated tumor cells] / P. F. Ambros, G. Méhes, Inge M. Ambros, Andrea Luegmayr, Ruth Ladenstein, H. Gadner
Dátum:2002
Megjegyzések:There are different reasons why the detection of disseminated tumor cells (DTCs) in the hematopoetic system is important. On the one hand the detection of disseminated tumor cells can provide vital information about a tumor's tendency to develop metastases. In some localized epithelial but also in embryonic tumors, for example a correlation between disseminated tumor cells and unfavorable outcome was observed (6, 14). These studies are based on the assumption that those tumor cells which appear in the hematopoetic system at a very early stage are responsible for the development of metastases. Another important aspect is the monitoring of the disease response to cytotoxic drugs by quantifying DTCs. During and after therapy there is no other possibility (except for an operation) to either directly analyze the effects the therapy has on the tumor cells or to determine their biological characteristics. The dissemination in the hematopoetic system, however, reveals the response to therapy and the biological features of the tumor cells. The prerequisites for such bone-marrow diagnosis, however, is the unequivocal identification of disseminated tumor cells. So in order to avoid false positive results (which are a risk in bone-marrow diagnostics), a system was developed to distinguish tumor cells from non-neoplastic cells and to facilitate insights into the biological make-up of tumor cells (2, 11)
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
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Megjelenés:Acta Medica Austriaca. Supplement. - 59 (2002), p. 58-61. -
További szerzők:Méhes Gábor (1966-) (patológus) Ambros, Ingeborg M. Luegmayr, Andrea Ladenstein, Ruth Gadner, Helmut
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM019458
Első szerző:Ambros, Peter F.
Cím:Unequivocal identification of disseminated tumor cells in the bone marrow by combining immunological and genetic approaches - functional and prognostic information / P. F. Ambros, G. Méhes, C. Hattinger, I. M. Ambros, A. Luegmayr, R. Ladenstein, H. Gadner
Dátum:2001
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Leukemia. - 15 : 2 (2001), p. 275-277. -
További szerzők:Méhes Gábor (1966-) (patológus) Hattinger, Claudia M. Ambros, Ingeborg M. Luegmayr, Andrea Ladenstein, Ruth Gadner, Helmut
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM019445
Első szerző:Ambros, Peter F.
Cím:Disseminated tumor cells in the bone marrow - chances and consequences of microscopical detection methods / Peter F. Ambros, Gabor Mehes, Inge M. Ambros, Ruth Ladenstein
Dátum:2003
Megjegyzések:The detection of disseminated tumor cells (DTCs) in the hematopoetic system is important for various reasons. It is essential for tumor staging. According to the International Neuroblastoma Staging System (INSS) only the cytomorphological examination of bone marrow smears is accepted despite the fact that an infiltrate below 0.1%, can hardly be detected and even infiltrates of more than 10% are sometimes overlooked. Another important aspect is the monitoring of the disease response to cytotoxic drugs by quantifying DTCs. Moreover, bone marrow aspirates represent an ideal source to determine the genetic and biological make up of DTCs at diagnosis and during follow up. Key issues that can be tested on DTCs are: determination of the proliferation capacity, the apoptotic rate, the drug sensitivity etc. The prerequisite for such a bone-marrow diagnosis, however, is the unequivocal identification of disseminated tumor cells. Thus, in order to avoid false positive and false negative results, which are a risk in bone-marrow diagnostics, a system was developed to distinguish tumor cells from non-neoplastic cells and to facilitate the gain of insights into the biological make-up of tumor cells more easily.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Cancer Letters. - 197 : 1-2 (2003), p. 29-34. -
További szerzők:Méhes Gábor (1966-) (patológus) Ambros, Ingeborg M. Ladenstein, Ruth
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM019455
Első szerző:Méhes Gábor (patológus)
Cím:Combined automatic immunological and molecular cytogenetic analysis allows exact identification and quantification of tumor cells in the bone marrow / Gábor Méhes, Andrea Luegmayr, Inge M. Ambros, Ruth Ladenstein, Peter F. Ambros
Dátum:2001
Megjegyzések:PURPOSE: To improve the detection of disseminated tumor cells in bone marrow (BM) and peripheral blood samples of solid tumor patients, a novel computer-assisted scanning system for automatic search, image analysis, and repositioning of these cells was developed. This system allows precise identification and quantification of tumor cells by sequential immunological and molecular cytogenetic analysis. In this study, we attempt to demonstrate the practical use of this approach by analyzing BM samples from neuroblastoma patients. EXPERIMENTAL DESIGN: The disialo-ganglioside (GD2) molecule was used as the immunological target. The GD2 molecule was described as being specific for neuroblastoma cells, although false positive reactions had been suspected. To verify or disprove the neoplastic nature of the immunologically positive cells, sequential fluorescence in situ hybridization was performed on these cells to search for those genetic aberrations found in the corresponding primary tumors. A total of 115 samples from 40 newly diagnosed patients were evaluated for the presence of GD2(+) cells in the BM. RESULTS: GD2 positivity was detected in 95.2% of stage 4 patients, in 100% of stage 4s patients, and in 38.5% of patients with localized/regional disease. In stage 4 and 4s BM samples, the GD2(+) cells were unequivocally identified as tumor cells based on the molecular cytogenetic aberrations found by fluorescence in situ hybridization. However, in BM samples from patients with localized/regional disease, all GD2(+) cells were concluded to represent false positivity due to the absence of genetic aberrations. CONCLUSIONS: Automatic search and sequential molecular cytogenetic analysis of the immunologically positive cells provide precise information on both the number and cytogenetic profile of disseminated tumor cells.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Clinical Cancer Research. - 7 : 7 (2001), p. 1969-1975. -
További szerzők:Luegmayr, Andrea Ambros, Ingeborg M. Ladenstein, Ruth Ambros, Peter F.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM019446
Első szerző:Méhes Gábor (patológus)
Cím:Detection of disseminated tumor cells in neuroblastoma : 3 log improvement in sensitivity by automatic immunofluorescence plus FISH (AIPF) analysis compared with classical bone marrow cytology / Gabor Mehes, Andrea Luegmayr, Rosa Kornmüller, Ingeborg M. Ambros, Ruth Ladenstein, Helmut Gadner, Peter F. Ambros
Dátum:2003
Megjegyzések:The sensitive detection of bone marrow involvement is crucial for tumor staging at diagnosis and for monitoring of the therapeutic response in the patient's follow-up. In neuroblastoma, only conventional cytomorphological techniques are presently accepted for the detection of bone marrow involvement, yet since the therapeutic consequences of the bone marrow findings may be far-reaching, the need for highly reliable detection methods has become evident. For this purpose, we developed an automatic immunofluorescence plus FISH (AIPF) device which allows the exact quantification of disseminated tumor cells and the genetic verification in critical cases. In this study, the power of the immunofluorescence technique is compared with conventional cytomorphology. 198 samples from 23 neuroblastoma patients (stages 4 and 4s) at diagnosis and during follow-up were investigated. At diagnosis, 45.6% of the samples (26 of 57) which were positive by AIPF investigation were negative by cytomorphology. During follow-up, 74.2% (49 of 66) of AIPF-positive samples showed no cytological signs of tumor cell involvement. False negative morphological results were found in up to 10% of tumor cell content. A tumor cell infiltrate below 0.1% was virtually not detectable by conventional cytomorphology. Using the sensitive immunofluorescence technique, the analysis of only two instead of four puncture sites did not lead to false negative results. Thus, the immunofluorescence technique offers an excellent tool for reliable detection and quantification of disseminated tumor cells at diagnosis and during the course of the disease
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:The American Journal of Pathology. - 163 : 2 (2003), p. 393-399. -
További szerzők:Luegmayr, Andrea Kornmüller, Rosa Ambros, Ingeborg M. Ladenstein, Ruth Gadner, Helmut Ambros, Peter F.
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM019672
Első szerző:Méhes Gábor (patológus)
Cím:Zellproliferation und Zelltod in disseminierten Tumorzellen / G. Méhes, Ruth Ladenstein, H. Gadner, P. F. Ambros
Dátum:2002
Megjegyzések:The occurrence of occult metastases of solid tumors at initial diagnosis or during follow-up is of crucial therapeutical importance. The sensitive detection of such cells in hematological samples depends on tissue specific cellular markers. The demonstration of minimally disseminated tumor cells at a given timepoint is, however, only a snapshot, which does not give any information about the potential and dynamics of the cells in question. Functional differences may fundamentally influence the impact of a positive finding. The analysis of cell proliferation and cell death (apoptosis) in disseminated tumor cells, for instance, defines, whether the dissemination process is progressive or regressive. With a newly developed automatic image analysis station the investigation of functional parameters in isolated cells from clinical samples became possible. The studies presented here demonstrate, that such techniques allow an improved identification of isolated tumor cells with clinical importance.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Acta Medica Austriaca. Supplement. - 59 (2002), p. 62-64. -
További szerzők:Ladenstein, Ruth Gadner, Helmut Ambros, Peter F.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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