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001-es BibID:	BIBFORM008847
Első szerző:	Ferencz Andrea
Cím:	Influence of PACAP on oxidative stress and tissue injury following small-bowel autotransplantation / Ferencz A., Racz B., Tamas A., Reglodi D., Lubics A., Nemeth J., Nedvig K., Kalmar-Nagy K., Horvath O. P., Weber G., Roth E.
Dátum:	2009
Megjegyzések:	Abstract Tissue injury caused by cold preservation and reperfusion remains an unsolved problem during smallbowel transplantation. Pituitary adenylate cyclase-activating polypeptide (PACAP) is present and plays a central role in the intestinal physiology. This study investigated effect of PACAP-38 on the oxidative stress and tissue damage in autotransplanted intestine. Sham-operated, ischemia/reperfusion, and autotransplanted groups were established in Wistar rats. In ischemia/reperfusion groups, 1 h (group A), 2 h (group B), and 3 h (group C) ischemia followed by 3 h of reperfusion was applied. In autotransplanted groups, total orthotopic intestinal autotransplantation was performed. Grafts were preserved in University of Wisconsin (UW) solution and in UW containing 30 ?g PACAP-38 for 1, 2, 3, and 6 h. Reperfusion lasted 3 h in all groups. Endogenous PACAP-38 concentration was measured by radioimmunoassay. To determine oxidative stress parameters, malondialdehyde, reduced glutathione, and superoxide dismutase were measured in tissue samples. Tissue damage was analyzed by qualitative and quantitative methods on hematoxylin/eosinstained sections. Concentration of endogenous PACAP-38 significantly decreased in groups B and C compared to sham-operated group. Preservation solution containing PACAP-38 ameliorated bowel tissue oxidative injury induced by cold ischemia and reperfusion. Histological results showed that preservation caused destruction of the mucous, submucous, and muscular layers, which were further deteriorated by the end of reperfusion. In contrast, PACAPJ-38 significantly protected the intestinal structure. Ischemia/ reperfusion decreased the endogenous PACAP-38 concentration in the intestinal tissue. Administration of PACAP-38 mitigated the oxidative injury and histological lesions in small-bowel autotransplantation model.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Small-bowel transplantation
Megjelenés:	Journal of Molecular Neuroscience. - 37 : 2 (2009), p. 168-176. -
További szerzők:	Rácz Boglárka Tamás Andrea (Idegtudomány) (Pécs) Reglődi Dóra (Idegtudományok) Lubics Andrea (Pécs) Németh József (1954-) (vegyész, analitikus) Nedvig Klára Kalmár-Nagy Károly Horváth Örs Péter (sebész) Wéber György Rőth Erzsébet (1957-) (vegyész)
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001-es BibID:	BIBFORM008851
Első szerző:	Ferencz A. (Pécs)
Cím:	Changes and effect of PACAP-38 on intestinal ischemia-reperfusion and Autotransplantation / A. Ferencz, B. Rácz, A. Tamás, K. Nedvig, J. Németh, K. Kalmár-Nagy, Ö. P. Horváth, Gy. Wéber, E. Röth, D. Reglödi
Dátum:	2009
ISSN:	0041-1345 (Print)
Megjegyzések:	Tissue injury caused by cold preservation and reperfusion during small bowel transplantation remains an unsolved problem. Increasing evidence suggests that pituitary adenylate cyclaseactivating polypeptide (PACAP) has protective effects in several ischemia-reperfusion (I/R) models. This study investigated the effect of PACAP-38 on oxidative stress in autotransplanted intestine. We established sham-operated, I/R, and autotransplanted groups in Wistar rats (n = 55). We applied ischemia for 1 (GI), 2 (GII), or 3 hours (GIII). In autotransplanted groups, we performed total orthotopic intestinal autotransplantation. Grafts were preserved in University of Wisconsin (UW) solution for 1 (GIV), 2 (GV), 3 (GVI), or 6 (GVII) hours and in PACAP-38-containing UW for 1 (GVIII), 2 (GIX), 3 (GX), or 6 (GXI) hours. Reperfusion lasted 3 hours in each group. Endogenous PACAP-38 values were measured by radioimmunoassay. Oxidative stress parameters malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) were measured in tissue homogenates. Concentration of endogenous PACAP-38 significantly decreased in GI to GIII compared with the sham-operated animals following I/R periods (P < .05). Cold preservation in UW and reperfusion of the intestine increased the level of tissue MDA in GIV to GVII, which correlated with the duration of cold storage. The content of GSH decreased in GIV to GVII to levels that were significantly different between GIV and GVIII and between GVII and GXI. SOD activity decreased dramatically in GIV to GVII with significantly higher activity in GIX to GXI. Our findings confirmed that I/R decreased endogenous PACAP-38 concentration. Administration of PACAP-38 to UW solution mitigated the oxidative injury during intestinal autotransplantation.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban PACAP-38 on Intestinal Ischemia-Reperfusion
Megjelenés:	Transplantation Proceedings. - 41 : 1 (2009), p. 57-59. -
További szerzők:	Rácz Boglárka Tamás A. (Pécs) Nedvig Klára Németh József (1954-) (vegyész, analitikus) Kalmár-Nagy Károly Horváth Örs Péter (sebész) Wéber György Rőth Erzsébet (1957-) (vegyész) Reglődi Dóra (Idegtudományok)
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