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1.

001-es BibID:BIBFORM008849
Első szerző:Börzsei Rita
Cím:Presence of pituitary adenylate cyclase activating polypeptide-38 in human plasma and milk / Rita Borzsei, Laszlo Mark, Andrea Tamas, Terez Bagoly, Csaba Bay, Katalin Csanaky, Eszter Banki, Peter Kiss, Alexandra Vaczy, Gabriella Horvath, Jozsef Nemeth, Edit Szauer, Zsuzsanna Helyes, Dora Reglodi
Dátum:2009
ISSN:0804-4643 (Print)
Megjegyzések:Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide widely distributed throughout the body. It is involved in the regulation of various physiological and pathophysiological processes, such as reproduction, thermoregulation, motor activity, brain development, neuronal survival, inflammation and pain. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma. Furthermore, based on the presence of vasoactive intestinal peptide, structurally the closest to PACAP, in milk and PACAP and its receptors in the mammary gland, our aim was to study PACAP-38 in human milk.Design and methods: The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20-40), as well as in plasma and milk samples from lactating women (age: 20-35). PACAP concentration was measured with a specific and sensitive RIA. Results: Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable in healthy volunteers and it is not significantly altered by gender, age, food intake or hormonal cycle in females. However, PACAP-38 plasma levels significantly increased in lactating women having 1-6 month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in the human milk with levels 5-20-fold greater in the milk whey than in the respective plasma samples. Conclusions: We found PACAP-38 in human plasma and its increase during the first 6 months of the lactation period. A prominent, nearly 10-fold higher concentration of this peptide was detected in human milk. Based on the literature, several important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer as well as regulation of growth/differentiation of certain tissues of the neonates. The novelty of the present descriptive data provides a basis for further investigations on the mechanism of PACAP-38 secretion in human milk and its functional significance.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
PACAP-38 human plasma, human milk
Megjelenés:European Journal of Endocrinology. - 160 : 4 (2009), p. 561-565. -
További szerzők:Márk László (1956-) (belgyógyász, kardiológus) Tamás Andrea (Idegtudomány) (Pécs) Bagoly Teréz Bay Csaba Csanaky Katalin Bánki Eszter Kiss Péter Váczy Alexandra Horváth Gabriella Németh József (1954-) (vegyész, analitikus) Szauer Edit Helyes Zsuzsanna Reglődi Dóra (Idegtudományok)
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2.

001-es BibID:BIBFORM021890
Első szerző:Czeglédi Levente (agrármérnök)
Cím:Presence of pituitary adenylate cyclase-activating polypeptide (PACAP) in the plasma and milk of ruminant animals / Czeglédi Levente, Tamás Andrea, Borzsei Rita, Bagoly Teréz, Kiss Péter, Horváth Gabriella, Brubel Réka, Németh József, Szalontai Bálint, Szabadfi Krisztina, Jávor András, Reglódi Dóra, Helyes Zsuzsanna
Dátum:2011
Megjegyzések:Milk contains a variety of proteins and peptides that possess biological activity. Growth factors, such as growth hormone, insulin-like, epidermal and nerve growth factors are important milk components which may regulate growth and differentiation in various neonatal tissues and also those of the mammary gland itself. We have recently shown that pituitary adenylate cyclase-activating polypeptide (PACAP), an important neuropeptide with neurotrophic actions, is present in the human milk in much higher concentration than in the plasma of lactating women. Investigation of growth factors in the milk of domestic animals is of utmost importance for their nutritional values and agricultural significance. Therefore, the aim of the present study was to determine the presence and concentration of PACAP in the plasma and milk of three ruminant animal species. Furthermore, the presence of PACAP and its specific PAC1 receptor were investigated in the mammary glands. Radioimmunoassay measurements revealed that PACAP was present in the plasma and the milk of the sheep, goat and the cow in a similar concentration to that measured previously in humans. PACAP38-like immunoreactivity (PACAP38-LI) was 5-20-fold higher in the milk than in the plasma samples of the respective animals, a similar serum/milk ratio was found in all the three species. The levels did not show significant changes within the examined 3-month-period of lactation after delivery. Similar PACAP38-LI was measured in the homogenates of the sheep mammary gland samples taken 7 and 30 days after delivery. PAC1 receptor expression was detected in these udder biopsies by fluorescent immunohistochemistry suggesting that this peptide might have an effect on the mammary glands themselves. These data show that PACAP is present in the milk of various ruminant domestic animal species at high concentrations, the physiological implications of which awaits further investigation.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:General and Comparative Endocrinology. - 172 : 1 (2011), p. 115-119. -
További szerzők:Tamás Andrea (Idegtudomány) (Pécs) Borzsei Rita (agrár) Bagoly Teréz Kiss Péter Horváth Gabriella Brubel Réka Németh József (1954-) (vegyész, analitikus) Szalontai Bálint (Pécsi Egyetem) Szabadfi Krisztina (Pécsi Egyetem) Jávor András (1952-) (agrármérnök) Reglődi Dóra (Idegtudományok) Helyes Zsuzsanna
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3.

001-es BibID:BIBFORM003869
Első szerző:Elekes Krisztián
Cím:Inhibitory effects of synthetic somatostatin receptor subtype 4 agonists on acute and chronic airway inflammation and hyperreactivity in the mouse / Krisztián Elekes, Zsuzsanna Helyes, László Kereskai, Katalin Sándor, Erika Pintér, Gábor Pozsgai, Valéria Tékus, Ágnes Bánvölgyi, József Németh, Tamás Szűts, György Kéri, János Szolcsányi
Dátum:2008
Megjegyzések:Somatostatin released fromactivated capsaicin-sensitive afferents of the lung inhibits inflammation and related bronchial hyperreactivity presumably via somatostatin 4 receptors (sst4). The aim of this studywas to examine the effects of TT-232, a heptapeptide sst4/sst1 receptor agonist and J-2156, a high affinity sst4 receptor-selective peptidomimetic agonist in airway inflammation models. Acute pneumonitis was evoked by intranasal lipopolysaccharide 24 h before measurement. Chronic inflammation was induced by ovalbumin inhalation on days 28, 29 and 30 after i.p. sensitization on days 1 and 14. Semiquantitative histopathological scoring was based on perivascular/peribronchial oedema, neutrophil/macrophage infiltration, goblet cell hyperplasia in the acute model and eosinophil infiltration,mucosal oedema,mucus production and epithelial cell damage in chronic inflammation.Myeloperoxidase activity of the lung was measured spectrophotometrically to quantify granulocyte accumulation and the broncoalveolar lavage fluid was analysed by flow cytometry. Carbachol-induced bronchoconstriction was assessed by unrestrained whole body plethysmography and its calculated indicator, enhanced pause (Penh)was determined. TT-232 and J-2156 induced similar inhibition on granulocyte recruitment and histopathological changes in both models, although macrophage infiltration in LPS-induced inflammation was unaltered by either compounds. Both agonists diminished inflammatory airway hyperresponsiveness. Since their single administration after the development of the inflammatory reactions also inhibited carbachol-induced bronchoconstriction, somatostatin sst4 receptor activation on bronchial smooth muscle cells is likely to be involved in their anti-hyperreactivity effect. These results suggest that stable, somatostatin sst4 receptor-selective agonists could be potential candidates for the development of a completely novel group of anti-inflammatory drugs for the treatment of airway inflammation and hyperresponsiveness.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Somatostatin sst4 receptor
Interleukin-1beta
Whole body plethysmography
Ovalbumin
Lipopolysaccharide
Bronchial hyperreactivity
Airway inflammation
Megjelenés:European Journal of Pharmacology. - 578 : 2-3 (2008), p. 313-322. -
További szerzők:Helyes Zsuzsanna Kereskai László Sándor Katalin Pintér Erika Pozsgai Gábor Tékus Valéria Bánvölgyi Ágnes Németh József (1954-) (vegyész, analitikus) Szűts Tamás Kéri György Szolcsányi János (Pécs)
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4.

001-es BibID:BIBFORM002463
Első szerző:Elekes Krisztián
Cím:Role of capsaicin-sensitive afferents and sensory neuropeptides / Krisztián Elekes, Zsuzsanna Helyes, József Németh, Katalin Sándor, Gábor Pozsgai, László Kereskai, Rita Börzsei, Erika Pintér, Árpád Szabó, János Szolcsányi
Dátum:2007
Megjegyzések:Substance P (SP) and calcitonin gene-related peptide (CGRP) released from capsaicin-sensitive afferents induce neurogenic inflammation via NK1, NK2 and CGRP1 receptor activation. This study examines the role of capsaicin-sensitive fibres and sensory neuropeptides in endotoxininduced airway inflammation and consequent bronchial hyperreactivity with functional, morphological and biochemical techniques in mice. Carbachol-induced bronchoconstriction was measured with whole body plethysmography 24 h after intranasal lipopolysaccharide administration. SP and CGRP were determined with radioimmunoassay, myeloperoxidase activity with spectrophotometry, interleukin-1? with ELISA and histopathological changes with semiquantitative scoring from lung samples. Treatments with resiniferatoxin for selective destruction of capsaicinsensitive afferents, NK1 antagonist SR 140333, NK2 antagonist SR 48968, their combination, or CGRP1 receptor antagonist CGRP(8-37) were performed. Lipopolysaccharide significantly increased lung SP and CGRP concentrations, which was prevented by resiniferatoxin pretreatment. Resiniferatoxin-desensitization markedly enhanced inflammation, but decreased bronchoconstriction. CGRP(8-37) or combination of SR 140333 and SR 48968 diminished neutrophil accumulation, MPO levels and IL-1? production, airway hyperresponsiveness was inhibited only by SR 48968. This is the first evidence that capsaicin-sensitive afferents exert a protective role in endotoxin-induced airway inflammation, but contribute to increased bronchoconstriction. Activation of CGRP1 receptors or NK1+NK2 receptors participate in granulocyte accumulation, but NK2 receptors play predominant role in enhanced airway resistance.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Whole body plethysmography
Myeloperoxidase activity
NK1 receptor
NK2 receptor
CGRP1 receptor
Somatostatin
Megjelenés:Regulatory Peptides. - 141 : 1-3 (2007), p. 44-54. -
További szerzők:Helyes Zsuzsanna Sándor Katalin Pozsgai Gábor Kereskai László Börzsei Rita Pintér Erika Szabó Árpád Szolcsányi János (Pécs) Németh József (1954-) (vegyész, analitikus)
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5.

001-es BibID:BIBFORM061062
Első szerző:Helyes Zsuzsanna
Cím:Pituitary Adenylate Cyclase-Activating Polypeptide Is Upregulated in Murine Skin Inflammation and Mediates Transient Receptor Potential Vanilloid-1-Induced Neurogenic Edema / Zsuzsanna Helyes, Jozsef Kun, Nora Dobrosi, Katalin Sándor, Jozsef Németh, Aniko Perkecz, Erika Pintér, Krisztina Szabadfi, Balazs Gaszner, Valeria Tékus, Janos Szolcsányi, Martin Steinhoff, Hitoshi Hashimoto, Dora Reglődi, Tamas Bíró
Dátum:2015
ISSN:0022-202X
Megjegyzések:Although pituitary adenylate cyclase-activating polypeptide (PACAP) was described as a key vasoregulator inhuman skin, little is known about its expression in mouse skin. As it is important to investigate PACAP signaling intranslational mouse dermatitis models, we determined its presence, regulation, and role in neurogenic and nonneurogeniccutaneous inflammatory mechanisms. The mRNA of PACAP and its specific receptor PAC1 wasdetected with real-time PCR in several skin regions at comparable levels. PACAP-38-immunoreactivity measuredwith radioimmunoassay was similar in plantar and dorsal paw skin and the ear but significantly smaller in theback skin. PACAP and PAC1 mRNA, as well as PACAP-38 and PAC1 protein expression, significantly increased in theplantar skin after intraplantar administration of capsaicin (50 ?l, 100 ?gml?1), an agonist of the transient receptorpotential vanilloid 1 (TRPV1) receptor, evoking chiefly neurogenic inflammation without inflammatory cellaccumulation. Intraplantar complete Freund's adjuvant (CFA; 50 ?l, 1mgml?1) also increased PACAP/PAC1 mRNAbut not the PACAP peptide. Capsaicin-induced neurogenic paw edema, but not CFA-evoked non-neurogenicswelling, was significantly smaller in PACAP-deficient mice throughout a 24-hour period. To our knowledge, weprovide previously unreported evidence for PACAP and PAC1 expression upregulation during skin inflammation ofdifferent mechanisms and for its pro-inflammatory function in neurogenic edema formation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
cross-talk
sebaceous gland
sebocytes
skin homeostasis
Megjelenés:Journal Of Investigative Dermatology 135 : 9 (2015), p. 2209-2218. -
További szerzők:Kun József Dobrosi Nóra (1981-) (molekuláris biológus) Sándor Katalin Németh József (1954-) (vegyész, analitikus) Perkecz Anikó Pintér Erika Szabadfi Krisztina (Pécsi Egyetem) Gaszner Balázs (Neuroanatómia) Tékus Valéria Szolcsányi János (Pécs) Steinhoff, Martin Hashimoto, Hitoshi Reglődi Dóra (Idegtudományok) Bíró Tamás (1968-) (élettanász)
Pályázati támogatás:OTKA-104984
OTKA
OTKA-PD100706
OTKA
OTKA-101761
OTKA
OTKA105369
OTKA
TÁMOP-4.2.2./A-11/1/KONV-2012-0025
TÁMOP
TÁMOP-4.2.4.A/ 2- 11/1-2012-0001
TÁMOP
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6.

001-es BibID:BIBFORM040213
Első szerző:Helyes Zsuzsanna
Cím:Effects of the somatostatin receptor subtype 4 selective agonist J-2156 on sensory neuropeptide release and inflammatory reactions in rodents / Z. Helyes, E. Pintér, J. Németh, K. Sándor, K. Elekes, A. Szabó, G. Pozsgai, D. Keszthelyi, L. Kereskai, M. Engström, S. Wurster, J. Szolcsányi
Dátum:2006
ISSN:0007-1188
Megjegyzések:Substance P (SP) and calcitonin gene-related peptide (CGRP) released from capsaicin-sensitivesensory nerves induce local neurogenic inflammation; somatostatin exerts systemic anti-inflammatory actions presumably viasst4/sst1 receptors. This study investigates the effects of a high affinity, sst4-selective, synthetic agonist, J-2156, on sensoryneuropeptide release in vitro and inflammatory processes in vivo.Experimental approach: Electrically-induced SP, CGRP and somatostatin release from isolated rat tracheae was measuredwith radioimmunoassay. Mustard oil-induced neurogenic inflammation in rat hindpaw skin was determined by Evans blueleakage and in the mouse ear with micrometry. Dextran-, carrageenan- or bradykinin-induced non-neurogenic inflammationwas examined with plethysmometry or Evans blue, respectively. Adjuvant-induced chronic arthritis was assessed byplethysmometry and histological scoring. Granulocyte accumulation was determined with myeloperoxidase assay and IL-1bwith ELISA.Key results: J-2156 (10-2000 nM) diminished electrically-evoked neuropeptide release in a concentration-dependentmanner. EC50 for the inhibition of substance P, CGRP and somatostatin release were 11.6 nM, 14.3nM and 110.7 nM,respectively. J-2156 (1-100 mgkg-1 i.p.) significantly, but not dose-dependently, inhibited neurogenic and non-neurogenicacute inflammatory processes and adjuvant-induced chronic oedema and arthritic changes. Endotoxin-evoked myeloperoxidaseactivity and IL-1b production in the lung, but not IL-1b- or zymosan-induced leukocyte accumulation in the skin weresignificantly diminished by J-2156.Conclusions and implications: J-2156 acting on sst4 receptors inhibits neuropeptide release, vascular components of acuteinflammatory processes, endotoxin-induced granulocyte accumulation and IL-1b synthesis in the lung and synovial andinflammatory cells in chronic arthritis. Therefore it might be a promising lead for the development of novel anti-inflammatorydrugs.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:British Journal Of Pharmacology. - 149 : 4 (2006), p. 405-415. -
További szerzők:Pintér E. Németh József (1954-) (vegyész, analitikus) Sándor K. Elekes K. Pozsgai Gábor Keszthelyi Dániel Kereskai László Engström, M. Wurster, S. Szolcsányi János (Pécs)
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7.

001-es BibID:BIBFORM020215
Első szerző:Helyes Zsuzsanna
Cím:Short-Term Fasting Differentially Alters / Z. Helyes, E. Pintér, J. Németh, K. Sándor, K. Elekes, Á. Szabó, G. Pozsgai, D. Keszthelyi, L. Kereskai, M. Engström, S. Wurster, J. Szolcsányi
Dátum:2006
Megjegyzések:The present article investigated the levels of pituitary adenylatecyclase-activating polypeptide (PACAP) and vasoactive intestinalpolypeptide (VIP) in the brains of rats and chickens 12, 36, and 84 hafter starvation. PACAP levels increased in both species, 12 h after fooddeprivation in rats, and with a 24-h delay in chickens. VIP levels showeda more complex pattern: a gradual increase in the hypothalamus andtelencephalon, and a significant decrease in the brain stem of rats. Inchickens, a decrease was observed in every brain area after 36 h of starvation.These data showthat PACAP and VIP are differentially regulatedand are involved in the regulatory processes under a food-restricted regimen,and are differentially altered in nocturnal and diurnal species.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
starvation
radioimmunoassay
hypothalamus
brain stem
Megjelenés:Annals of the New York Academy of Sciences. - 1070 (2006), p. 354-358. -
További szerzők:Pintér E. Németh József (1954-) (vegyész, analitikus) Sándor K. Elekes K. Szabó Á. Pozsgai Gábor Keszthelyi Dániel Kereskai László Engström, M. Wurster, S. Szolcsányi János (Pécs)
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8.

001-es BibID:BIBFORM002467
Első szerző:Helyes Zsuzsanna
Cím:Inhibitory effect of PACAP-38 on acute neurogenic and non-neurogenic inflammatory processes in the rat / Z. Helyes, G. Pozsgai, R. Börzsei , J. Németh, T. Bagoly, L. Márk, E. Pintér, G. Tóth, K. Elekes, J. Szolcsányi, D. Reglodi
Dátum:2007
Megjegyzések:Inhibitory actions of pituitary adenylate cyclase activating polypeptide (PACAP) have been described on cellular/vascular inflammatory components, but there are few data concerning its role in neurogenic inflammation. In this study we measured PACAP-like immunoreactivity with radioimmunoassay in the rat plasma and showed a two-fold elevation in response to systemic stimulation of capsaicin-sensitive sensory nerves by resiniferatoxin, but not after local excitation of cutaneous afferents. Neurogenic plasma extravasation in the plantar skin induced by intraplantar capsaicin or resiniferatoxin, as well as carrageenaninduced paw edema were significantly diminished by intraperitoneal PACAP-38. In summary, these results demonstrate that PACAP is released from activated capsaicin-sensitive afferents into the systemic circulation. It diminishes acute pure neurogenic and mixed-type inflammatory reactions via inhibiting pro-inflammatory mediator release and/or by acting at post-junctional targets on the vascular endothelium.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Capsaicin-sensitive sensory nerves
Megjelenés:Peptides. - 28 : 9 (2007), p. 1847-1855. -
További szerzők:Pozsgai Gábor Börzsei Rita Bagoly Teréz Márk László (1956-) (belgyógyász, kardiológus) Pintér Erika Tóth G. Elekes Krisztián Szolcsányi János (Pécs) Reglődi Dóra (Idegtudományok) Németh József (1954-) (vegyész, analitikus)
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9.

001-es BibID:BIBFORM002464
Első szerző:Helyes Zsuzsanna
Cím:Role of transient receptor potential vanilloid 1 receptors in endotoxin-induced / Zsuzsanna Helyes, Krisztián Elekes, József Németh, Gábor Pozsgai, Katalin Sándor, László Kereskai, Rita Börzsei, Erika Pintér, Árpád Szabó, János Szolcsányi
Dátum:2007
Megjegyzések:Airways are densely innervated by capsaicin-sensitive sensory neurons expressing transient receptor potential vanilloid 1 (TRPV1) receptors/ion channels, which play an important regulatory role in inflammatory processes via the release of sensory neuropeptides. The aim of the present study was to investigate the role of TRPV1 receptors in endotoxin-induced airway inflammation and consequent bronchial hyperreactivity with functional, morphological, and biochemical techniques using receptor gene-deficient mice. Inflammation was evoked by intranasal administration of Escherichia coli lipopolysaccharide (60 gammal, 167 gammag/ml) in TRPV1 knockout (TRPV1-/-) mice and their wild-type counterparts (TRPV1+/+) 24 h before measurement. Airway reactivity was assessed by unrestrained whole body plethysmography, and its quantitative indicator, enhanced pause (Penh), was calculated after inhalation of the bronchoconstrictor carbachol. Histological examination and spectrophotometric myeloperoxidase measurement was performed from the lung. Somatostatin concentration was measured in the lung and plasma with radioimmunoassay. Bronchial hyperreactivity, histological lesions (perivascular/peribronchial edema, neutrophil/ macrophage infiltration, goblet cell hyperplasia), and myeloperoxidase activity were significantly greater in TRPV-/- mice. Inflammation markedly elevated lung and plasma somatostatin concentrations in TRPV1+/+ but not TRPV1-/- animals. In TRPV1-/- mice, exogenous administration of somatostatin-14 (4 x 100 gamma g/kg ip) diminished inflammation and hyperreactivity. Furthermore, in wildtype mice, antagonizing somatostatin receptors by cyclo-somatostatin (4 x 250 gamma g/kg ip) increased these parameters. This study provides the first evidence for a novel counterregulatory mechanism during endotoxin-induced airway inflammation, which is mediated by somatostatin released from sensory nerve terminals in response to activation of TRPV1 receptors of the lung. It reaches the systemic circulation and inhibits inflammation and consequent bronchial hyperreactivity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
capsaicin-sensitive afferents
inflammatory airway hyperreactivity
Megjelenés:American journal of physiology. Lung cellular and molecular physiology. - 292 (2007), p. L1173-L1181. -
További szerzők:Sándor Katalin Szolcsányi János (Pécs) Szabó Árpád Pintér Erika Börzsei Rita Kereskai László Pozsgai Gábor Elekes Krisztián Németh József (1954-) (vegyész, analitikus)
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10.

001-es BibID:BIBFORM013279
Első szerző:Horváth Gabriella
Cím:Mice deficient in pituitary adenylate cyclase activating polypeptide display increased sensitivity to renal oxidative stress in vitro / Gabriella Horvath, Laszlo Mark, Reka Brubel, Peter Szakaly, Boglarka Racz, Peter Kiss, Andrea Tamas, Zsuzsanna Helyes, Andrea Lubics, Hitoshi Hashimoto, Akemichi Baba, Norihito Shintani, Gergely Furjes, Jozsef Nemeth, Dora Reglodi
Dátum:2010
Megjegyzések:Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional neuropeptide, showingwidespread occurrence in the nervous system and also in peripheral organs. The neuroprotective effectsof PACAP are well-established in different neuronal systems against noxious stimuli in vitro and in vivo.Recently, its general cytoprotective actions have been recognized, including renoprotective effects. However,the effect of endogenous PACAP in the kidneys is not known. The main aim of the present studywas to investigate whether the lack of this endogenous neuropeptide influences survival of kidney cellsagainst oxidative stress. First, we determined the presence of endogenous PACAP from mouse kidneyhomogenates by mass spectrometry and PACAP-like immunoreactivity by radioimmunoassay. Second,primary cultures were isolated from wild type and PACAP deficient mice and cell viability was assessedfollowing oxidative stress induced by 0.5, 1.5 and 3mM H2O2. Our mass spectrometry and radioimmunoassayresults show that PACAP is endogenously present in the kidney. The main part of our studyrevealed that the sensitivity of cells from PACAP deficient mice was increased to oxidative stress: bothafter 2 or 4 h of exposure, cell viability was significantly reduced compared to that from control wild typemice. This increased sensitivity of kidneys from PACAP deficient mice could be counteracted by exogenouslygiven PACAP38. These results show, for the first time, that endogenous PACAP protects againstoxidative stress in the kidney, and that PACAP may act as a stress sensor in renal cells. These findingsfurther support the general cytoprotective nature of this neuropeptide.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Cell viability
Oxidative stress
PACAP knockout mice
Renoprotection
Megjelenés:Neuroscience Letters. - 469 : 1 (2010), p. 70-74. -
További szerzők:Márk László (1956-) (belgyógyász, kardiológus) Brubel Réka Szakály Péter Rácz Boglárka Kiss Péter Tamás Andrea (Idegtudomány) (Pécs) Helyes Zsuzsanna Lubics Andrea (Pécs) Hashimoto, Hitoshi Baba, Akemichi Shintani Norihito Fürjes Gergely Németh József (1954-) (vegyész, analitikus) Reglődi Dóra (Idegtudományok)
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11.

001-es BibID:BIBFORM020274
Első szerző:Horváth Péter
Cím:Changes in tracheo-bronchial sensory neuropeptide receptor gene expression pattern in rats with cisplatin-induced sensory neuropathy / Péter Horváth, Zoltán Szilvássy, Barna Peitl, Judit Szilvássy, Zsuzsanna Helyes, János Szolcsányi, József Németh
Dátum:2006
ISSN:0143-4179
Megjegyzések:AbstractAn attenuated neurogenic broncho-constriction underpinned by a decrease in sensory neuropeptide release has been shown to be characteristic of cisplatin-induced neuropathy. The present work was to explore if beyond neuropeptide release, cisplatin at a treatment schedule attaining sensory neuropathy, produced changes in the expression of the receptors of sensory neuropeptides such as somatostatin, calcitonin gene-related peptide (CGRP) and substance P (SP) in bronchial tissue of the rat. Twenty-four Wistar rats were divided into three groups. The animals in the "Treatment groups 1 and 2" were given cisplatin (1.5mgkg(-1)) and mannitol (75mgkg(-1)) over 5 days. The rats in the "Control" group were given mannitol+isotonic saline. Four animals from each group were used to study the expression pattern of the neuropeptide receptors in bronchial tissue. The levels of somatostatin receptor 4 (SSTR 4), neurokinin 1 (NK1), neurokinin 2 (NK2) and CGRP receptor expression were examined by quantitative real time polymerase chain reaction (RT-PCR) method, 11 and 22 days after the last cisplatin/vehicle dose. The cisplatin treatment significantly increased plasma somatostatin immunoreactivity and the expression of SSTR4 receptor detected both on the 11th and 22nd post-treatment days with no change in either CGRP, NK1, and NK2 receptor gene expression or plasma CGRP and substance P levels. We conclude that cisplatin neuropathy is accompanied by an increase in plasma somatostatin immunoreactivity with an increase in SSTR4 expression in rats.
Tárgyszavak:Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
tracheo-bronchial
neuropeptide receptor
cisplatin-induced
Megjelenés:Neuropeptides. - 40 : 1 (2006), p. 77-83. -
További szerzők:Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus) Peitl Barna (1972-) (orvos, farmakológus) Szilvássy Judit (1960-2022) (fül- orr- gégész) Helyes Zsuzsanna Szolcsányi János (Pécs) Németh József (1954-) (vegyész, analitikus)
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DOI
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Borító:

12.

001-es BibID:BIBFORM087705
Első szerző:Kiss Péter (Pécs)
Cím:Presence of PACAP-38 in mammalian plasma and milk : from guinea pig to humans / Kiss P., Csanaky K., Bánki E., Tamás A., Börzsei R., Márk L., Bagoly T., Bay Cs., Váczy A., Horváth G., Németh J., Czeglédi L., Szauer E., Helyes Zs., Reglődi D.
Dátum:2010
ISSN:0231-424X 1588-2683
Megjegyzések:PACAP is a pleiotropic and multifunctional neuropeptide widely distributed throughout the body. It is involved in the regulation of various physiological and pathophysiological processes, such as reproduction, thermoregulation, motor activity, brain development, neuronal survival, inflammation and pain. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma. Furthermore, based on the presence of VIP, structurally the closest to PACAP, in milk, and presence of PACAP- and PACAP receptor-immunoreactive elements in the mammary gland, our aim was also to examine PACAP-38 in human milk. For comparative studies, we investigated presence of PACAP in plasma and milk of other species, including goat, sheep, cow and guinea pig. Methods. The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20?40), in plasma and milk samples from lactating women (age: 20?35). PACAP concentration was measured with a specific and sensitive radioimmunoassay in human and other mammalian samples. Results. Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable in healthy volunteers and it is not significantly altered by gender, age, food intake or the hormonal cycle in females. However, PACAP-38 plasma levels significantly increased in lactating women having 1?6-month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in the human milk with levels 5?20-fold greater in the milk whey than in the respective plasma samples. The same ratio was found in all other examined species. Conclusions. Based on the present findings and the literature, several important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer as well as regulation of growth/differentiation of certain tissues of the neonates.
Tárgyszavak:Orvostudományok Egészségtudományok idézhető absztrakt
folyóiratcikk
PACAP-38
Megjelenés:Acta Physiologica Hungarica. - 97 : 1 (2010), p. 115. -
További szerzők:Csanaky Katalin Bánki Eszter Tamás Andrea (Idegtudomány) (Pécs) Börzsei Rita Márk László Bagoly Teréz Bay Csaba Váczy Alexandra Horváth Gabriella Németh József (1954-) (vegyész, analitikus) Czeglédi Levente (1977-) (agrármérnök) Szauer Edit Helyes Zsuzsanna Reglődi Dóra (Idegtudományok)
Pályázati támogatás:K72592
OTKA
K73044
OTKA
F67830
OTKA
78480
OTKA
K75965
OTKA
Gedeon Richter Centenary Foundation
Egyéb
Janos Bolyai Postdoctoral Research Fellowship
Egyéb
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