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1.

001-es BibID:BIBFORM008849
Első szerző:Börzsei Rita
Cím:Presence of pituitary adenylate cyclase activating polypeptide-38 in human plasma and milk / Rita Borzsei, Laszlo Mark, Andrea Tamas, Terez Bagoly, Csaba Bay, Katalin Csanaky, Eszter Banki, Peter Kiss, Alexandra Vaczy, Gabriella Horvath, Jozsef Nemeth, Edit Szauer, Zsuzsanna Helyes, Dora Reglodi
Dátum:2009
ISSN:0804-4643 (Print)
Megjegyzések:Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide widely distributed throughout the body. It is involved in the regulation of various physiological and pathophysiological processes, such as reproduction, thermoregulation, motor activity, brain development, neuronal survival, inflammation and pain. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma. Furthermore, based on the presence of vasoactive intestinal peptide, structurally the closest to PACAP, in milk and PACAP and its receptors in the mammary gland, our aim was to study PACAP-38 in human milk.Design and methods: The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20-40), as well as in plasma and milk samples from lactating women (age: 20-35). PACAP concentration was measured with a specific and sensitive RIA. Results: Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable in healthy volunteers and it is not significantly altered by gender, age, food intake or hormonal cycle in females. However, PACAP-38 plasma levels significantly increased in lactating women having 1-6 month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in the human milk with levels 5-20-fold greater in the milk whey than in the respective plasma samples. Conclusions: We found PACAP-38 in human plasma and its increase during the first 6 months of the lactation period. A prominent, nearly 10-fold higher concentration of this peptide was detected in human milk. Based on the literature, several important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer as well as regulation of growth/differentiation of certain tissues of the neonates. The novelty of the present descriptive data provides a basis for further investigations on the mechanism of PACAP-38 secretion in human milk and its functional significance.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
PACAP-38 human plasma, human milk
Megjelenés:European Journal of Endocrinology. - 160 : 4 (2009), p. 561-565. -
További szerzők:Márk László (1956-) (belgyógyász, kardiológus) Tamás Andrea (Idegtudomány) (Pécs) Bagoly Teréz Bay Csaba Csanaky Katalin Bánki Eszter Kiss Péter Váczy Alexandra Horváth Gabriella Németh József (1954-) (vegyész, analitikus) Szauer Edit Helyes Zsuzsanna Reglődi Dóra (Idegtudományok)
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2.

001-es BibID:BIBFORM002463
Első szerző:Elekes Krisztián
Cím:Role of capsaicin-sensitive afferents and sensory neuropeptides / Krisztián Elekes, Zsuzsanna Helyes, József Németh, Katalin Sándor, Gábor Pozsgai, László Kereskai, Rita Börzsei, Erika Pintér, Árpád Szabó, János Szolcsányi
Dátum:2007
Megjegyzések:Substance P (SP) and calcitonin gene-related peptide (CGRP) released from capsaicin-sensitive afferents induce neurogenic inflammation via NK1, NK2 and CGRP1 receptor activation. This study examines the role of capsaicin-sensitive fibres and sensory neuropeptides in endotoxininduced airway inflammation and consequent bronchial hyperreactivity with functional, morphological and biochemical techniques in mice. Carbachol-induced bronchoconstriction was measured with whole body plethysmography 24 h after intranasal lipopolysaccharide administration. SP and CGRP were determined with radioimmunoassay, myeloperoxidase activity with spectrophotometry, interleukin-1? with ELISA and histopathological changes with semiquantitative scoring from lung samples. Treatments with resiniferatoxin for selective destruction of capsaicinsensitive afferents, NK1 antagonist SR 140333, NK2 antagonist SR 48968, their combination, or CGRP1 receptor antagonist CGRP(8-37) were performed. Lipopolysaccharide significantly increased lung SP and CGRP concentrations, which was prevented by resiniferatoxin pretreatment. Resiniferatoxin-desensitization markedly enhanced inflammation, but decreased bronchoconstriction. CGRP(8-37) or combination of SR 140333 and SR 48968 diminished neutrophil accumulation, MPO levels and IL-1? production, airway hyperresponsiveness was inhibited only by SR 48968. This is the first evidence that capsaicin-sensitive afferents exert a protective role in endotoxin-induced airway inflammation, but contribute to increased bronchoconstriction. Activation of CGRP1 receptors or NK1+NK2 receptors participate in granulocyte accumulation, but NK2 receptors play predominant role in enhanced airway resistance.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Whole body plethysmography
Myeloperoxidase activity
NK1 receptor
NK2 receptor
CGRP1 receptor
Somatostatin
Megjelenés:Regulatory Peptides. - 141 : 1-3 (2007), p. 44-54. -
További szerzők:Helyes Zsuzsanna Sándor Katalin Pozsgai Gábor Kereskai László Börzsei Rita Pintér Erika Szabó Árpád Szolcsányi János (Pécs) Németh József (1954-) (vegyész, analitikus)
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3.

001-es BibID:BIBFORM002467
Első szerző:Helyes Zsuzsanna
Cím:Inhibitory effect of PACAP-38 on acute neurogenic and non-neurogenic inflammatory processes in the rat / Z. Helyes, G. Pozsgai, R. Börzsei , J. Németh, T. Bagoly, L. Márk, E. Pintér, G. Tóth, K. Elekes, J. Szolcsányi, D. Reglodi
Dátum:2007
Megjegyzések:Inhibitory actions of pituitary adenylate cyclase activating polypeptide (PACAP) have been described on cellular/vascular inflammatory components, but there are few data concerning its role in neurogenic inflammation. In this study we measured PACAP-like immunoreactivity with radioimmunoassay in the rat plasma and showed a two-fold elevation in response to systemic stimulation of capsaicin-sensitive sensory nerves by resiniferatoxin, but not after local excitation of cutaneous afferents. Neurogenic plasma extravasation in the plantar skin induced by intraplantar capsaicin or resiniferatoxin, as well as carrageenaninduced paw edema were significantly diminished by intraperitoneal PACAP-38. In summary, these results demonstrate that PACAP is released from activated capsaicin-sensitive afferents into the systemic circulation. It diminishes acute pure neurogenic and mixed-type inflammatory reactions via inhibiting pro-inflammatory mediator release and/or by acting at post-junctional targets on the vascular endothelium.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Capsaicin-sensitive sensory nerves
Megjelenés:Peptides. - 28 : 9 (2007), p. 1847-1855. -
További szerzők:Pozsgai Gábor Börzsei Rita Bagoly Teréz Márk László (1956-) (belgyógyász, kardiológus) Pintér Erika Tóth G. Elekes Krisztián Szolcsányi János (Pécs) Reglődi Dóra (Idegtudományok) Németh József (1954-) (vegyész, analitikus)
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4.

001-es BibID:BIBFORM002464
Első szerző:Helyes Zsuzsanna
Cím:Role of transient receptor potential vanilloid 1 receptors in endotoxin-induced / Zsuzsanna Helyes, Krisztián Elekes, József Németh, Gábor Pozsgai, Katalin Sándor, László Kereskai, Rita Börzsei, Erika Pintér, Árpád Szabó, János Szolcsányi
Dátum:2007
Megjegyzések:Airways are densely innervated by capsaicin-sensitive sensory neurons expressing transient receptor potential vanilloid 1 (TRPV1) receptors/ion channels, which play an important regulatory role in inflammatory processes via the release of sensory neuropeptides. The aim of the present study was to investigate the role of TRPV1 receptors in endotoxin-induced airway inflammation and consequent bronchial hyperreactivity with functional, morphological, and biochemical techniques using receptor gene-deficient mice. Inflammation was evoked by intranasal administration of Escherichia coli lipopolysaccharide (60 gammal, 167 gammag/ml) in TRPV1 knockout (TRPV1-/-) mice and their wild-type counterparts (TRPV1+/+) 24 h before measurement. Airway reactivity was assessed by unrestrained whole body plethysmography, and its quantitative indicator, enhanced pause (Penh), was calculated after inhalation of the bronchoconstrictor carbachol. Histological examination and spectrophotometric myeloperoxidase measurement was performed from the lung. Somatostatin concentration was measured in the lung and plasma with radioimmunoassay. Bronchial hyperreactivity, histological lesions (perivascular/peribronchial edema, neutrophil/ macrophage infiltration, goblet cell hyperplasia), and myeloperoxidase activity were significantly greater in TRPV-/- mice. Inflammation markedly elevated lung and plasma somatostatin concentrations in TRPV1+/+ but not TRPV1-/- animals. In TRPV1-/- mice, exogenous administration of somatostatin-14 (4 x 100 gamma g/kg ip) diminished inflammation and hyperreactivity. Furthermore, in wildtype mice, antagonizing somatostatin receptors by cyclo-somatostatin (4 x 250 gamma g/kg ip) increased these parameters. This study provides the first evidence for a novel counterregulatory mechanism during endotoxin-induced airway inflammation, which is mediated by somatostatin released from sensory nerve terminals in response to activation of TRPV1 receptors of the lung. It reaches the systemic circulation and inhibits inflammation and consequent bronchial hyperreactivity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
capsaicin-sensitive afferents
inflammatory airway hyperreactivity
Megjelenés:American journal of physiology. Lung cellular and molecular physiology. - 292 (2007), p. L1173-L1181. -
További szerzők:Sándor Katalin Szolcsányi János (Pécs) Szabó Árpád Pintér Erika Börzsei Rita Kereskai László Pozsgai Gábor Elekes Krisztián Németh József (1954-) (vegyész, analitikus)
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5.

001-es BibID:BIBFORM087705
Első szerző:Kiss Péter (Pécs)
Cím:Presence of PACAP-38 in mammalian plasma and milk : from guinea pig to humans / Kiss P., Csanaky K., Bánki E., Tamás A., Börzsei R., Márk L., Bagoly T., Bay Cs., Váczy A., Horváth G., Németh J., Czeglédi L., Szauer E., Helyes Zs., Reglődi D.
Dátum:2010
ISSN:0231-424X 1588-2683
Megjegyzések:PACAP is a pleiotropic and multifunctional neuropeptide widely distributed throughout the body. It is involved in the regulation of various physiological and pathophysiological processes, such as reproduction, thermoregulation, motor activity, brain development, neuronal survival, inflammation and pain. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma. Furthermore, based on the presence of VIP, structurally the closest to PACAP, in milk, and presence of PACAP- and PACAP receptor-immunoreactive elements in the mammary gland, our aim was also to examine PACAP-38 in human milk. For comparative studies, we investigated presence of PACAP in plasma and milk of other species, including goat, sheep, cow and guinea pig. Methods. The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20?40), in plasma and milk samples from lactating women (age: 20?35). PACAP concentration was measured with a specific and sensitive radioimmunoassay in human and other mammalian samples. Results. Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable in healthy volunteers and it is not significantly altered by gender, age, food intake or the hormonal cycle in females. However, PACAP-38 plasma levels significantly increased in lactating women having 1?6-month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in the human milk with levels 5?20-fold greater in the milk whey than in the respective plasma samples. The same ratio was found in all other examined species. Conclusions. Based on the present findings and the literature, several important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer as well as regulation of growth/differentiation of certain tissues of the neonates.
Tárgyszavak:Orvostudományok Egészségtudományok idézhető absztrakt
folyóiratcikk
PACAP-38
Megjelenés:Acta Physiologica Hungarica. - 97 : 1 (2010), p. 115. -
További szerzők:Csanaky Katalin Bánki Eszter Tamás Andrea (Idegtudomány) (Pécs) Börzsei Rita Márk László Bagoly Teréz Bay Csaba Váczy Alexandra Horváth Gabriella Németh József (1954-) (vegyész, analitikus) Czeglédi Levente (1977-) (agrármérnök) Szauer Edit Helyes Zsuzsanna Reglődi Dóra (Idegtudományok)
Pályázati támogatás:K72592
OTKA
K73044
OTKA
F67830
OTKA
78480
OTKA
K75965
OTKA
Gedeon Richter Centenary Foundation
Egyéb
Janos Bolyai Postdoctoral Research Fellowship
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM013278
Első szerző:Reglődi Dóra (Idegtudományok)
Cím:Alterations of pituitary adenylate cyclase-activating polypeptide-like immunoreactivity in the human plasma during pregnancy and after birth / D. Reglodi, J. Gyarmati, T. Ertl, R. Borzsei, J. Bodis, A. Tamas, P. Kiss, K. Csanaky, E. Banki, C. Bay, J. Nemeth, Z. Helyes
Dátum:2010
Megjegyzések:Pituitary adenylate cyclase-activating polypeptide (PACAP)was discovered from the hypothalamus and it belongs tothe vasoactive intestinal peptide (VIP)/secretin/glucagonspeptide family (1). It occurs in two biologically active forms,with 38 and 27 amino-acid residues. The first studies reportedon its adenylate cyclase activation in pituitary cellsand described its distribution in hypothalamic nuclei influencingpituitary hormone secretion (1). Although the last 20years since its discovery have revealed numerous distincteffects in the nervous system and various peripheral organs,its endocrine functions still represent a major focusin PACAP research. PACAP influences, among others, therelease of GnRH and FSH in different species (2, 3) andthe secretion of PRL (4-6). PACAP also influences sexualbehavior, ovarian follicular maturation, and fertility (3, 7,8), uteroplacental circulation (9), and trophoblast survival(10). Furthermore, PACAP is a very important trophic factor,especially during the development of the nervous system(1, 11). Radioimmunoassay (RIA) analysis revealed thatPACAP38 concentration is higher in the hypophyseal portalblood than in the peripheral circulation in the rat (12). Inspite of the vast literature on PACAP, there are only fewhuman studies. The presence of PACAP has been shownin human blood and some other tissues (1, 13). The half-lifeof PACAP in the circulation is relatively short, only a fewminutes, due to rapid enzymatic degradation by dipeptidyl-peptidase IV (14). We have recently shown the presenceof PACAP38 in human milk by mass spectrometry,and we have also found that PACAP38-like immunoreactivity(PACAP38-LI) is 5-20 times higher in the milk than inthe plasma (15). In humans, PACAP has also been shownto influence PRL, vasopressin, and oxytocin secretion (5,16). Immunohistochemical studies have revealed the presenceof PACAP and its receptors in the female reproductivesystem, including the placenta, uterus, and mammarygland (9, 17). Recent studies have suggested the involvementof PACAP in the pathophysiological processes ofstress urinary incontinence and the prolapse of pelvic organsin female patients (18).Regarding the role of PACAP in reproductive functionsand the urging need to obtain further human data, theaim of the present study was to investigate whether theconcentration of PACAP is altered during normal pregnancy,delivery, and after childbirth. We also measuredthe levels of endogenous PACAP with RIA in the peripheralblood and of newborn babies, as well as in the bloodobtained from the umbilical arteries and veins.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Delivery
newborn
PACAP
RIA
umbilical vessels
Megjelenés:Journal of Endocrinological Investigation. - 33 : 7 (2010), p. 443-445. -
További szerzők:Gyarmati János (1926-1983) (radiológus) Ertl Tibor Börzsei Rita Bodis József (Pécs) Tamás A. (Pécs) Kiss Péter (Pécs) Csanaky Katalin Bánki Eszter Bay Csaba Németh József (1954-) (vegyész, analitikus) Helyes Zsuzsanna
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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7.

001-es BibID:BIBFORM003875
Első szerző:Reglődi Dóra (Idegtudományok)
Cím:Agonistic behavior of PACAP6-38 on sensory nerve terminals and cytotrophoblast cells / D. Reglodi, R. Borzsei, T. Bagoly, A. Boronkai, B. Racz, A. Tamas, P. Kiss, G. Horvath, R. Brubel, J. Nemeth, G. Toth, Z. Helyes
Dátum:2008
Megjegyzések:The effects of pituitary adenylate cyclase activating polypeptide (PACAP) are mediated through Gprotein-coupled receptors, the specific PAC1 receptor and VPAC1 and VPAC2 receptors which bind vasoactive intestinal peptide with similar affinity. Based on binding affinity studies, PACAP6-38 was discovered as a potent antagonist of PAC1 and it has been used by hundreds of studies as a PACAP antagonist. Recently, we have found that in certain cells/tissues, PACAP6-38 does not antagonize PACAP-induced effects, but surprisingly, it exerts similar actions to PACAP1-38, behaving as an agonist. In the present study, we report on the agonistic behavior of PACAP6-38 on neuropeptide release from sensory nerves of the isolated rat trachea and on the MAPK signaling pathways in cytotrophoblast cells. In isolated rat tracheae, PACAP6-38, similarly to PACAP1-38, induced significant inhibitory effects on the release of three simultaneously measured sensory neuropeptides, substance P, calcitonin gene-related peptide, and somatostatin evoked by both chemical excitation and electrical field stimulation of capsaicin-sensitive afferents. Effects of PACAP6-38 were the same as those of PACAP1-38 on MAPK signaling in human cytotrophoblast cells. Western blot analysis showed that both peptide forms stimulated ERK1/2 and JNK phosphorylation, while they both inhibited p38 MAPK phosphorylation. The most pronounced effects were observed when both peptides were present. In summary, our results show that PACAP6-38, which is a PACAP receptor antagonist in most cells/tissues, can behave as an agonist in other systems. The increasing interest in the effects of PACAP requires further studies on the pharmacological properties of the peptide and its analogues.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
PACAP fragment
Analogue
Somatostatin
Substance P
JAR cytotrophoblast
CGRP
Megjelenés:Journal of Molecular Neuroscience. - 36 : 1-3 (2008), p. 270-278. -
További szerzők:Börzsei Rita Bagoly Teréz Boronkai A. Rácz Boglárka Tamás Andrea (Idegtudomány) (Pécs) Kiss Péter Horváth G. Brubel Réka Tóth Gábor Helyes Zsuzsanna Németh József (1954-) (vegyész, analitikus)
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