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001-es BibID:	BIBFORM031294
Első szerző:	Brubel Réka
Cím:	Investigation of pituitary adenylate cyclase activating polypeptide in human gynecological and other biological fluids by using MALDI TOF mass spectrometry. / Brubel R., Reglodi D., Jambor E., Koppan M., Varnagy A., Biro Z., Kiss P., Gaal V., Bodis J., Bay Cs., Veszpremi B., Tamas A., Nemeth J., Mark L.
Dátum:	2011
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional and pleiotropic neuropeptide. PACAP hasdiverse effects in the endocrine system, among others, it plays important roles in oogenesis, implantation and development ofthe nervous system.However, it is not knownwhether PACAP is present in the fluids of the human reproductive organs. The aimof the present study was to determine, by means of mass spectrometry and radioimmunoassay, whether PACAP is present inhuman amniotic fluid, ovarian follicular fluid and cervico-vaginal fluid. Samples were obtained from healthy adult volunteers.Our MALDI TOF and MALDI TOF/TOF spectrometry results show that PACAP38 is present in all of the follicular fluid samples,and PACAP-like immonoreactivity was also measured by radioimmunoassay. However, we did not find the characteristic peakrepresenting the unmodified 38 amino acid form of the peptide in normal cervico-vaginal smear and amniotic fluid samples.Furthermore, we analyzed other body fluids for comparison, such as human nasal fluid, saliva and aqueous humor. PACAPwas not found in these latter samples. In summary, the present study provides evidence for the presence of PACAP in humanfollicular fluid, suggesting a role in oocyte function, but determination of the exact physiological significance awaits furtherinvestigation.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban cervico-vaginal fluid amniotic fluid follicular fluid nasal fluid saliva aqueous humor mass spectrometry radioimmunoassay
Megjelenés:	Journal of Mass Spectrometry. - 46 : 2 (2011), p. 189-194. -
További szerzők:	Reglődi Dóra (Idegtudományok) Jámbor E. Koppán Miklós Várnagy A. Biró Zsolt (szemész) Kiss P. Gaál V. Bodis József (Pécs) Bay Csaba Veszprémi B. Tamás A. (Pécs) Németh József (1954-) (vegyész, analitikus) Mark L.
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM002473
Első szerző:	Kiss P.
Cím:	Changes of PACAP levels in the brain show gender differences following short-term water and food deprivation / P. Kiss, D. Reglödi, A. Tamás, A. Lubics, I. Lengvári, R. Józsa, A. Somogyvári-Vigh, Z. Szilvássy, J. Németh
Dátum:	2007
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic neuropeptide exerting diverse actions in the central and peripheral nervous systems. A few studies indicate that PACAP is involved in the regulation of feeding and water homeostasis. The aim of the present study was to investigate changes in PACAP38 concentrations in diVerent brain areas following food or water deprivation in male and female rats. Rats were sacriWced 12, 36 and 84 h after water or food removal. PACAP levels were determined by radioimmunoassay. Our results show that levels of PACAP decreased in the hypothalamus in both sexes after water deprivation, with a more marked, signiWcant decrease in females at 12 h. A decrease was observed also in the telencephalon, with a similar pattern in both genders: levels were lowest after 12 h, and showed a gradual increase at the other two time-points. PACAP levels increased in the brainstem of male rats, while females had a decrease 12 h after water deprivation. The pattern of changes in PACAP levels was very diVerent after food deprivation. In male rats, PACAP levels showed a signiWcant increase in the hypothalamus, telencephalon and brainstem 12 h after the beginning of starvation. In females, a less marked increase was observed only in the hypothalamus while no changes were found in the other brain areas. Our results show a sensitive reaction in changes of endogenous PACAP levels to water and food deprivation in most brain areas, but they are diVerentially regulated in male and female rats.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Gender differences RIA Starvation CNS
Megjelenés:	General and Comparative Endocrinology. - 152 : 2-3 (2007), p. 225-230. -
További szerzők:	Józsa Rita (Pécs) Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus) Németh József (1954-) (vegyész, analitikus) Reglődi Dóra (Idegtudományok) Tamás Andrea (Idegtudomány) (Pécs) Lubics Andrea (Pécs) Lengvári István Somogyvári-Vigh Anikó
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001-es BibID:	BIBFORM003049
Első szerző:	Németh József (vegyész, analitikus)
Cím:	PACAP-27 radioimmunoassay : description and application of a novel method / J. Nemeth, B. Jakab, R. Jozsa, T. Hollossy, A. Tamas, A. Lubics, I. Lengvari, P. Kiss, Zs. Oberritter, B. Horvath, Z. Szilvassy, D. Reglődi
Dátum:	2007
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény hazai lapban PACAP-27 radioimmunoassay
Megjelenés:	Journal of Radioanalytical and Nuclear Chemistry. - 273 : 2 (2007), p. 327-332. -
További szerzők:	Jakab Balázs (Pécs) Józsa Rita (Pécs) Hollósy Tibor (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Lubics Andrea (Pécs) Lengvári István Kiss P. Oberritter Zsolt Horváth B. Reglődi Dóra (Idegtudományok) Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus)
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001-es BibID:	BIBFORM042640
035-os BibID:	PMID:22648511
Első szerző:	Szántó Zoltán
Cím:	PACAP immunoreactivity in human malignant tumor samples and cardiac diseases / Z. Szanto, Zs. Sarszegi, D. Reglodi, J. Nemeth, K. Szabadfi, P. Kiss, A. Varga, E. Banki, K. Csanaky, B. Gaszner, O. Pinter, Zs. Szalai, A. Tamas
Dátum:	2013
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide(PACAP) is a pleiotropic and multifunctional neuropeptidehaving important roles in various physiological processes.Recent trends in PACAP research point to the clinical introductionof PACAP or its analogs/fragments possibly in thenear future. Recently, we have shown the presence ofPACAP in human plasma, milk, placenta, and follicularfluid samples. However, relatively few data are availableon PACAP in human tissues from patients with differentdisorders. The aim of the present study was to determine, byradioimmunoassay, the tissue level of PACAP38-like immunoreactivity(LI) and PACAP27-LI in different primary nonsmallcell lung cancer, colon tumor samples, and in cardiacmuscle samples from patients suffering from ischemic heartdisease and valvular disorders. We also labeled the PAC1receptors in human cardiac cells. All samples showed significantlyhigher PACAP38-LI compared with PACAP27-LI. We found significantly lower levels of PACAP38-LI andPACAP27-LI in tumoral and peripheral samples comparedwith normal healthy tissue in both lung and colon cancers.Further investigations are necessary to describe the exactfunction of PACAP in oncogenesis. We showed thatPACAP38-LI and PACAP27-LI are significantly higher inischemic heart diseases compared with valvular abnormalities,suggesting that PACAP might play a role in ischemicheart disorders.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban PACAP Colon cancer Lung cancer Heart disorder PAC1 receptor egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal of Molecular Neuroscience. - 48 : 3 (2013), p. 667-673. -
További szerzők:	Sárszegi Zsolt Reglődi Dóra (Idegtudományok) Németh József (1954-) (vegyész, analitikus) Szabadfi Krisztina (Pécsi Egyetem) Kiss P. Varga A. (anatómia, Pécs) Bánki Eszter Csanaky Katalin Gaszner Balázs (Neuroanatómia) Pintér Olivér Szalai Zsuzsanna Tamás A. (Pécs)
Pályázati támogatás:	OTKA-75965 OTKA OTKA-72592 OTKA
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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