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001-es BibID:	BIBFORM031291
Első szerző:	Brubel Réka
Cím:	Presence of pituitary adenylate cyclase activating polypeptide and its type I receptor in the rat kidney / Brubel R., Horvath G., Reglodi D., Lubics A., Tamas A., Kiss P., Laszlo E., Nemeth J., Mark L., Szakaly P.
Dátum:	2011
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP), a multifunctional neuropeptide,has 2 active forms, PACAP38 and PACAP27. It is now well-established that PACAPhas several actions also in peripheral organs, including renoprotective effects. The peptideitself has not been previously identified in the rat kidney. The first aim of our study was toidentify PACAP in the rat kidney using mass spectrometry and radioimmunoassay (RIA).Receptor mRNA and binding studies revealed the existence of all 3 PACAP receptors(PAC1, VPAC1, and VPAC2) in the kidney, but their exact localization in histologicsections was not evident. Because most of the cytoprotective effects of PACAP relate to itsspecific PAC1 receptor, our second aim was to identify the cell types wherein the PAC1receptor is expressed in the rat kidney. Mass spectrometry revealed the presence ofPACAP38 in the kidney. RIA measurements showed both PACAP38- and PACAP27-likeimmunoreactivities in kidney homogenates, with PACAP38 being dominant. Immunohistochemistryrevealed PAC1 receptor-like immunoreactivity in kidney sections, mainlyexpressed in cortical tubular epithelial cells. These results showed PACAP to beendogenously present in the kidney. The tubular localization of the PAC1 receptorprovides the basis for the renal effects of the peptide under physiologic and pathologicconditions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban PACAP38 PACAP27 rat kidney receptor
Megjelenés:	Transplantation Proceedings. - 43 : 4 (2011), p. 1297-1299. -
További szerzők:	Horváth Gábor (Pécs) Reglődi Dóra (Idegtudományok) Lubics Andrea (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Kiss Péter (Pécs) László E. Németh József (1954-) (vegyész, analitikus) Mark L. Szakály Péter
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM013279
Első szerző:	Horváth Gabriella
Cím:	Mice deficient in pituitary adenylate cyclase activating polypeptide display increased sensitivity to renal oxidative stress in vitro / Gabriella Horvath, Laszlo Mark, Reka Brubel, Peter Szakaly, Boglarka Racz, Peter Kiss, Andrea Tamas, Zsuzsanna Helyes, Andrea Lubics, Hitoshi Hashimoto, Akemichi Baba, Norihito Shintani, Gergely Furjes, Jozsef Nemeth, Dora Reglodi
Dátum:	2010
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional neuropeptide, showingwidespread occurrence in the nervous system and also in peripheral organs. The neuroprotective effectsof PACAP are well-established in different neuronal systems against noxious stimuli in vitro and in vivo.Recently, its general cytoprotective actions have been recognized, including renoprotective effects. However,the effect of endogenous PACAP in the kidneys is not known. The main aim of the present studywas to investigate whether the lack of this endogenous neuropeptide influences survival of kidney cellsagainst oxidative stress. First, we determined the presence of endogenous PACAP from mouse kidneyhomogenates by mass spectrometry and PACAP-like immunoreactivity by radioimmunoassay. Second,primary cultures were isolated from wild type and PACAP deficient mice and cell viability was assessedfollowing oxidative stress induced by 0.5, 1.5 and 3mM H2O2. Our mass spectrometry and radioimmunoassayresults show that PACAP is endogenously present in the kidney. The main part of our studyrevealed that the sensitivity of cells from PACAP deficient mice was increased to oxidative stress: bothafter 2 or 4 h of exposure, cell viability was significantly reduced compared to that from control wild typemice. This increased sensitivity of kidneys from PACAP deficient mice could be counteracted by exogenouslygiven PACAP38. These results show, for the first time, that endogenous PACAP protects againstoxidative stress in the kidney, and that PACAP may act as a stress sensor in renal cells. These findingsfurther support the general cytoprotective nature of this neuropeptide.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Cell viability Oxidative stress PACAP knockout mice Renoprotection
Megjelenés:	Neuroscience Letters. - 469 : 1 (2010), p. 70-74. -
További szerzők:	Márk László (1956-) (belgyógyász, kardiológus) Brubel Réka Szakály Péter Rácz Boglárka Kiss Péter Tamás Andrea (Idegtudomány) (Pécs) Helyes Zsuzsanna Lubics Andrea (Pécs) Hashimoto, Hitoshi Baba, Akemichi Shintani Norihito Fürjes Gergely Németh József (1954-) (vegyész, analitikus) Reglődi Dóra (Idegtudományok)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM040248
035-os BibID:	PMID:16888211
Első szerző:	Szakály Péter
Cím:	Changes in PACAP levels in the central nervous system after ovariectomy and castration / P. Szakaly, G. Horvath, P. Kiss, E. Laszlo, J. Farkas, G. Furjes, J. Nemeth, D. Reglodi
Dátum:	2006
ISSN:	0077-8923
Megjegyzések:	The aim of the present article was to investigate the influenceof gonadectomy on pituitary adenylate cyclase-activating polypeptide(PACAP) levels in different brain areas. In males, there seems tobe an inverse relationship between gonadotropins and PACAP in thebrain in the acute phase of castration: PACAP levels decreased in almostall brain areas examined within the first week after castration. Infemales, such pattern was observed in the hypothalamus, brain stem,and temporal cortex. In the pituitary, levels decreased only on the firstday after ovariectomy, and later, as in the thalamus, increases were observed.Although the pattern of change showed gender differences, ourresults provide further evidence that levels of gonadotropins and possiblygonadotropin-releasing hormone influence PACAP levels and thatPACAP is involved in the regulation of gonadal functions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Annals of the New York Academy of Sciences. - 1070 (2006), p. 468-473. -
További szerzők:	Németh József (1954-) (vegyész, analitikus) Horváth Gábor (Pécs) Kiss Péter (Pécs) László E. Farkas J. (Pécs) Fürjes Gergely Reglődi Dóra (Idegtudományok)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM013280
Első szerző:	Szakály Péter
Cím:	Changes in Pituitary Adenylate Cyclase-Activating Polypeptide Following Renal Ischemia-Reperfusion in Rat / P. Szakaly, G. Horvath, P. Kiss, E. Laszlo, J. Farkas, G. Furjes, J. Nemeth, D. Reglodi
Dátum:	2010
Megjegyzések:	PACAP (pituitary adenylate cyclase-activating polypeptide) occurs in two biologicallyactive forms, with 38 and 37 amino acid residues (PACAP38 and PACAP27). Inmammalian tissues, PACAP38 is the dominant form. Diverse effects have been describedin the cardiovascular, respiratory, gastrointestinal, and urogenital systems. PACAP isknown for its strong cytoprotective effects, which are present endogenously as well, asproven by knockout studies and results showing that PACAP is up-regulated followingdiverse injuries. Little is known about such effects in the kidney. We have previously shownthat PACAP is protective in renal ischemia-reperfusion injury. Therefore, the aim of thepresent study was to investigate the changes of endogenous PACAP following 60-minuterenal ischemia using radioimmunoassay. Changes were observed within 24 hours followingrenal vessel clamping. In the cortex, an acute decrease was followed by an increase on theintact side, and levels returned to original ones on the operated side. In the medulla,changes were only observed on the clamped side: a marked up-regulation was detected inPACAP38-like immunoreactivity within the first 24 hours. The present study showed thatPACAP38- and PACAP27-like immunoreactivities sensitively react to renal ischemiareperfusion,the physiological importance of which awaits further investigation.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban PACAP38 and PACAP27
Megjelenés:	Transplantation Proceedings. - 42 : 6 (2010), p. 2283-2286. -
További szerzők:	Horváth Gábor (Pécs) Kiss Péter (Pécs) László E. Farkas J. (Pécs) Fürjes Gergely Németh József (1954-) (vegyész, analitikus) Reglődi Dóra (Idegtudományok)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat DOI
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