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001-es BibID:BIBFORM013274
Első szerző:Brubel Réka
Cím:Changes in the Expression of Pituitary Adenylate Cyclase-Activating Polypeptide in the Human Placenta during Pregnancy and Its Effects on the Survival of JAR Choriocarcinoma Cells/ R. Brubel, A. Boronkai, D. Reglodi, B. Racz, J. Nemeth, P. Kiss, A. Lubics, G. Toth, G. Horvath, T. Varga, D. Szogyi, E. Fonagy, J. Farkas, A. Barakonyi, Sz. Bellyei, L. Szereday, M. Koppan, A. Tamas
Dátum:2010
Megjegyzések:Pituitary adenylate cyclase-activating polypeptide(PACAP), a neuropeptide with survival-promotingactions, has been observed in endocrine organs and isthought to play a role in reproductive functions, includingpregnancy. PACAP occurs in two forms, 27 and 38 aminoacid residues, with PACAP38 being the predominant formin human tissues. In the present study, we determined theconcentrations of PACAP38 and PACAP27 in firsttrimesterand full-term human placentas using radioimmunoassay.We found high levels of PACAP38 and lowerlevels of PACAP27 in different parts of the full-term humanplacenta. PACAP38 content increased in the placentaduring pregnancy, both on the maternal side and on thefetal side. The effects of PACAP on the survival of JARhuman choriocarcinoma cells were investigated using flowcytometry and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cell viability assay in cellsexposed to the widely used chemotherapeutic agentmethotrexate (MTX). It was found that PACAP neitherinfluenced the survival of JAR cytotrophoblast cells noraffected cellular response to the death-inducing effect of thechemotherapeutic agent MTX. The present observationsfurther support the significance of PACAP in the humanplacenta. The observation that PACAP did not influence theeffects of MTX may have future clinical importance,showing that PACAP does not decrease the effects ofcertain chemotherapeutic agents.Keywords JAR . Choriocarcinoma . RIA .
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
JAR
Choriocarcinoma
RIA
PACAP38
Megjelenés:Journal of Molecular Neuroscience. - 42 : 3 (2010), p. 450-458. -
További szerzők:Boronkai A. Reglődi Dóra (Idegtudományok) Rácz B. (Pécs) Németh József (1954-) (vegyész, analitikus) Kiss Péter (Pécs) Lubics Andrea (Pécs) Tóth Gábor (Szeged) Horváth Gábor (Pécs) Varga T. (Pécs) Szogyi D. (Pécs) Fonagy E. Farkas J. (Pécs) Barakonyi A. Bellyei Szabolcs Szereday László Koppán Miklós Tamás A. (Pécs)
Internet cím:DOI
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2.

001-es BibID:BIBFORM003875
Első szerző:Reglődi Dóra (Idegtudományok)
Cím:Agonistic behavior of PACAP6-38 on sensory nerve terminals and cytotrophoblast cells / D. Reglodi, R. Borzsei, T. Bagoly, A. Boronkai, B. Racz, A. Tamas, P. Kiss, G. Horvath, R. Brubel, J. Nemeth, G. Toth, Z. Helyes
Dátum:2008
Megjegyzések:The effects of pituitary adenylate cyclase activating polypeptide (PACAP) are mediated through Gprotein-coupled receptors, the specific PAC1 receptor and VPAC1 and VPAC2 receptors which bind vasoactive intestinal peptide with similar affinity. Based on binding affinity studies, PACAP6-38 was discovered as a potent antagonist of PAC1 and it has been used by hundreds of studies as a PACAP antagonist. Recently, we have found that in certain cells/tissues, PACAP6-38 does not antagonize PACAP-induced effects, but surprisingly, it exerts similar actions to PACAP1-38, behaving as an agonist. In the present study, we report on the agonistic behavior of PACAP6-38 on neuropeptide release from sensory nerves of the isolated rat trachea and on the MAPK signaling pathways in cytotrophoblast cells. In isolated rat tracheae, PACAP6-38, similarly to PACAP1-38, induced significant inhibitory effects on the release of three simultaneously measured sensory neuropeptides, substance P, calcitonin gene-related peptide, and somatostatin evoked by both chemical excitation and electrical field stimulation of capsaicin-sensitive afferents. Effects of PACAP6-38 were the same as those of PACAP1-38 on MAPK signaling in human cytotrophoblast cells. Western blot analysis showed that both peptide forms stimulated ERK1/2 and JNK phosphorylation, while they both inhibited p38 MAPK phosphorylation. The most pronounced effects were observed when both peptides were present. In summary, our results show that PACAP6-38, which is a PACAP receptor antagonist in most cells/tissues, can behave as an agonist in other systems. The increasing interest in the effects of PACAP requires further studies on the pharmacological properties of the peptide and its analogues.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
PACAP fragment
Analogue
Somatostatin
Substance P
JAR cytotrophoblast
CGRP
Megjelenés:Journal of Molecular Neuroscience. - 36 : 1-3 (2008), p. 270-278. -
További szerzők:Börzsei Rita Bagoly Teréz Boronkai A. Rácz Boglárka Tamás Andrea (Idegtudomány) (Pécs) Kiss Péter Horváth G. Brubel Réka Tóth Gábor Helyes Zsuzsanna Németh József (1954-) (vegyész, analitikus)
Internet cím:elektronikus változat
DOI
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