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001-es BibID:	BIBFORM003867
Első szerző:	Hernádi László
Cím:	The presence and distribution of pituitary adenylate cyclase activating polypeptide and its receptor in the snail helix pomatia / L. Hernádi, Z. Pirger, T. Kiss, J. Németh, L. Mark, P. Kiss, A. Tamas, A. Lubics, G. Toth, S. Shioda, D. Reglodi
Dátum:	2008
Megjegyzések:	The aim of this study was to show the presence, distribution and function of the pituitary adenylate cyclase activating polypeptide (PACAP) and its receptors in the CNS and peripheral nervous system of the mollusk, Helix pomatia. PACAP-like and pituitary adenylate cyclase activating polypeptide receptor (PAC1-R)-like immunoreactivity was abundant both in the CNS and the peripheral nervous system of the snail. In addition several non-neuronal cells also revealed PACAP-like immunoreactivity. In inactive animals labeled cell bodies were mainly found and in the neuropile of active animals dense immunostained fiber system was additionally detected suggesting that expression of PACAP-like peptide was affected by the behavioral state of the animal. RIA measurements revealed the existence of both forms of PACAP in the CNS where the 27 amino acid form was found to be dominant. The concentration of PACAP27 was significantly higher in samples from active animals supporting the data obtained by immunohistochemistry. In Western blot experiments PACAP27 and PACAP38 antibodies specifically labeled protein band at 4.5 kDa both in rat and snail brain homogenates, and additionally an 14 kDa band in snail. The 4.5 kDa protein corresponds to PACAP38 and the 14 kDa protein corresponds to the preproPACAP or to a PACAP-like peptide having larger molecular weight than mammalian PACAP38. In matrix-assisted laser desorption ionization time of flight (MALDI TOF) measurements fragments of PACAP38 were identified in brain samples suggesting the presence of a large molecular weight peptide in the snail. Applying antibodies developed against the PACAP receptor PAC1-R, immunopositive stained neurons and a dense network of fibers were identified in each of the ganglia. In electrophysiological experiments, extracellular application of PACAP27 and PACAP38 transiently depolarized or increased postsynaptic activity of neurons expressing PAC1-R. In several neurons PACAP elicited a long lasting hyperpolarization which was eliminated after 1.5 h continuous washing. Taken together, these results indicate that PACAP may have significant role in a wide range of basic physiological functions in snail.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban pituitary adenylate cyclase activating polypeptide,
Megjelenés:	Neuroscience. - 155 : 2 (2008), p. 387-402. -
További szerzők:	Pirger Zsolt Kiss Tibor Németh József (1954-) (vegyész, analitikus) Márk László (1956-) (belgyógyász, kardiológus) Kiss Péter Tamás Andrea (Idegtudomány) (Pécs) Lubics Andrea (Pécs) Tóth Gábor Shioda, Seiji Reglődi Dóra (Idegtudományok)
Internet cím:	elektronikus változat DOI
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001-es BibID:	BIBFORM003874
Első szerző:	Pirger Zsolt
Cím:	PACAP has anti-apoptotic effect in the salivary gland of an invertebrate species, helix pomatia / Zsolt Pirger, Jozsef Nemeth, Laszlo Hiripi, Gabor Toth, Peter Kiss, Andrea Lubics, Andrea Tamas, Laszlo Hernadi, Tibor Kiss, Dora Reglodi
Dátum:	2008
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP) shows a remarkable sequence similarity among species and several studies provide evidence that the functions of PACAP have also been conserved among vertebrate species. Relatively little is known about its presence and functions in invertebrates. The aim of the present study was to investigate whether the well-known anti-apoptotic effect of PACAP can also be demonstrated in invertebrates. This effect was studied in the salivary gland of a molluscan species, Helix pomatia. In this work, we first showed the presence of PACAP-like immunoreactivity in the Helix salivary gland by means of immunohistochemistry. Radioimmunoassay measurements showed that PACAP38-like immunoreactivity dominated in the salivary gland of both active and inactive snails and its concentration was higher in active than in inactive animals in contrast to PACAP27-like immunoreactivity, which did not show activity-dependent changes. PACAP induced a significant elevation of cAMP level in salivary gland extracts. Application of apoptosis-inducing agents, dopamine and colchicine, led to a marked increase in the number of terminal uridine deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive apoptotic cells in the salivary gland, which was significantly attenuated by PACAP treatment. In a similar manner, the number of caspase-positive cells was reduced after co-application of dopamine and PACAP. Taken together, the data indicate that PACAP activates cAMP in a molluscan species and we show, for the first time, that PACAP is anti-apoptotic in the invertebrate Helix pomatia.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Caspase-3 TUNEL RIA cAMP Apoptosis
Megjelenés:	Journal of molecular neuroscience. - 36 : 1-3 (2008), p. 105-114. -
További szerzők:	Németh József (1954-) (vegyész, analitikus) Hiripi László Tóth Gábor Kiss Péter Lubics Andrea (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Hernádi László Kiss Tibor Reglődi Dóra (Idegtudományok)
Internet cím:	elektronikus változat DOI
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