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001-es BibID:	BIBFORM003873
Első szerző:	Boros Ákos
Cím:	Changes in the expression of PACAP-like compounds during the embryonic development of the earthworm eisenia fetida / Akos Boros, Dora Reglodi, Zsofia Herbert, Gabor Kiszler, Jozsef Nemeth, Andrea Lubics, Peter Kiss, Andrea Tamas, Seiji Shioda, Kouhei Matsuda, Edit Pollak, Laszló Molnar
Dátum:	2008
Megjegyzések:	Pituitary adenylate cyclase-activating polypeptide (PACAP) is expressed at very early stages in the vertebrate nervous system, and its functions in the embryonic development have been shown by various studies. PACAP is an extremely conserved molecule in phylogeny; however, little is known about its presence and functions in invertebrates. Our previous studies have shown the occurrence of PACAP-like immunoreactivity in the invertebrate nervous system. The aim of this study was to investigate the presence and localization of PACAP-like compounds during the embryonic development of earthworms from cocoon deposition to hatching using immunological methods (radioimmunoassay, dot blot, immunohistochemistry). PACAP-like immunoreactive compounds were detected at very early stages of the embryonic development of the earthworm Eisenia fetida. No significant changes were observed during the early stages in the developing embryo, but a marked increase occurred before hatching. In contrast, during the embryonic development, the level of PACAP-like compounds gradually decreased in cocoon fluids. Immunohistochemistry revealed the presence of PACAP-like immunoreactive cell bodies and processes in the developing body wall, prostomium, pharyngeal wall, and central nervous system. Cells located in the body wall correspond to putative progenitor cells of primary sensory cells. In the present study, we also showed that the clitellum (reproductive organ) of sexually mature worms contained significantly higher levels of PACAP-like immunoreactivity than other regions of the same animals or the clitellar region of a non-reproducing animal. In summary, these observations provide a morphological basis and suggest a role of PACAP(-like peptides) in the reproductive and developmental functions of invertebrates.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Dot blot Clitellum Cocoon Earthworm embryo Primary sensory cells Radioimmunoassay
Megjelenés:	Journal of molecular neuroscience. - 36 : 1-3 (2008), p. 157-165. -
További szerzők:	Reglődi Dóra (Idegtudományok) Herbert Zsófia Kiszler Gábor Németh József (1954-) (vegyész, analitikus) Lubics Andrea (Pécs) Kiss Péter Tamás Andrea (Idegtudomány) (Pécs) Shioda, Seiji Matsuda, Kouhei Pollák Edit Molnár László
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001-es BibID:	BIBFORM008850
Első szerző:	Somogyi Ildikó
Cím:	Pituitary Adenylate Cyclase-activating Polypeptide-like Compounds Could Modulate the Activity of Coelomocytes in the Earthworm / Ildiko Somogyi, Akos Boros, Peter Engelmann, Eszter Varhalmi, Jozsef Nemeth, Andrea Lubics, Andrea Tamas, Peter Kiss, Dora Reglodi, Edit Pollak, Laszlo Molnar
Dátum:	2009
Megjegyzések:	By means of radioimmunoassay, we studied the concentration of pituitary adenylate cyclase-activating polypeptide (PACAP)-like proteins in intact and regenerating earthworms. Transection of animals increased the concentration of PACAP-like compounds in coelomocytes, and a decreasing rostrocaudal gradient was detected in the regenerating animals. Western blot analysis revealed a range of PAC1-receptor proteins with molecular weights from 40 to 80 kDa. Electron microscopic immunocytochemistry showed that PAC1 receptors were located on distinct sets of coelomocytes (mainly on amebocytes and on some granulocytes). Based on our results we hypothesize a link between PACAP and coelomocytes, suggesting that PACAP modulates the function of amebocytes and certain granulocytes that play a role in tissue remodeling of regenerating earthworms.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban radioimmunoassay Western blot immunocytochemistry Eisenia fetida
Megjelenés:	Annals of the New York Academy of Sciences. - 1163 (2009), p. 521-523. -
További szerzők:	Boros Ákos Engelmann, Peter Várhalmi Eszter Németh József (1954-) (vegyész, analitikus) Lubics Andrea (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Kiss Péter Reglődi Dóra (Idegtudományok) Pollák Edit Molnár László
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