Találati lista | Tudóstér

001-es BibID:	BIBFORM003873
Első szerző:	Boros Ákos
Cím:	Changes in the expression of PACAP-like compounds during the embryonic development of the earthworm eisenia fetida / Akos Boros, Dora Reglodi, Zsofia Herbert, Gabor Kiszler, Jozsef Nemeth, Andrea Lubics, Peter Kiss, Andrea Tamas, Seiji Shioda, Kouhei Matsuda, Edit Pollak, Laszló Molnar
Dátum:	2008
Megjegyzések:	Pituitary adenylate cyclase-activating polypeptide (PACAP) is expressed at very early stages in the vertebrate nervous system, and its functions in the embryonic development have been shown by various studies. PACAP is an extremely conserved molecule in phylogeny; however, little is known about its presence and functions in invertebrates. Our previous studies have shown the occurrence of PACAP-like immunoreactivity in the invertebrate nervous system. The aim of this study was to investigate the presence and localization of PACAP-like compounds during the embryonic development of earthworms from cocoon deposition to hatching using immunological methods (radioimmunoassay, dot blot, immunohistochemistry). PACAP-like immunoreactive compounds were detected at very early stages of the embryonic development of the earthworm Eisenia fetida. No significant changes were observed during the early stages in the developing embryo, but a marked increase occurred before hatching. In contrast, during the embryonic development, the level of PACAP-like compounds gradually decreased in cocoon fluids. Immunohistochemistry revealed the presence of PACAP-like immunoreactive cell bodies and processes in the developing body wall, prostomium, pharyngeal wall, and central nervous system. Cells located in the body wall correspond to putative progenitor cells of primary sensory cells. In the present study, we also showed that the clitellum (reproductive organ) of sexually mature worms contained significantly higher levels of PACAP-like immunoreactivity than other regions of the same animals or the clitellar region of a non-reproducing animal. In summary, these observations provide a morphological basis and suggest a role of PACAP(-like peptides) in the reproductive and developmental functions of invertebrates.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Dot blot Clitellum Cocoon Earthworm embryo Primary sensory cells Radioimmunoassay
Megjelenés:	Journal of molecular neuroscience. - 36 : 1-3 (2008), p. 157-165. -
További szerzők:	Reglődi Dóra (Idegtudományok) Herbert Zsófia Kiszler Gábor Németh József (1954-) (vegyész, analitikus) Lubics Andrea (Pécs) Kiss Péter Tamás Andrea (Idegtudomány) (Pécs) Shioda, Seiji Matsuda, Kouhei Pollák Edit Molnár László
Internet cím:	elektronikus változat DOI
Borító:
Saját polcon:

001-es BibID:	BIBFORM003871
Első szerző:	Várhalmi Eszter
Cím:	Expression of PACAP-Like compounds during the caudal regeneration of the earthworm eisenia fetida / Eszter Varhalmi, Ildiko Somogyi, Gabor Kiszler, Jozsef Nemeth, Dora Reglodi, Andrea Lubics, Peter Kiss, Andrea Tamas, Edit Pollak, Laszlo Molnar
Dátum:	2008
Megjegyzések:	The regeneration of the ventral nerve cord ganglion and peripheral tissues was investigated by radioimmunoassay and immunohistochemistry in the model animal, Eisenia fetida (Annelida, Oligochaeta). It is now well-established that pituitary adenylate cyclase-activating polypeptide (PACAP) is a neurotrophic factor, playing important roles in the development of the nervous system in vertebrate animals. Based on the apparent evolutionary conservation of PACAP and on the several common mechanisms of vertebrate and invertebrate nervous regeneration, the question was raised whether PACAP has any role in the regeneration of the earthworm nervous system. As a first step, we studied the distribution, concentration, and time-course of PACAP-like immunoreactivity during caudal regeneration of both lost segments and the ventral nerve cord ganglia in E. fetida. A strong upregulation of PACAP-like immunoreactivity was observed in most tissues following injury as determined by radioimmunoassay and immunohistochemistry. Significant increases in the concentration of PACAP-like compounds were found in the body wall, alimentary canal, and in coelomocytes. The most characteristic morphological feature was the accumulation of immunolabeled neoblasts in the injured tissues, especially in the ventral nerve cord ganglion that initiates and mediates regeneration processes. Our present results show that PACAP/PACAP-like peptides accumulate in the regenerating tissues of the earthworm, suggesting trophic functions of these compounds in earthworm tissues similarly to vertebrate species.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Annelida Coelomocyte Neoblast RIA Regeneration Regeneration blastema
Megjelenés:	Journal of Molecular Neuroscience. - 36 : 1-3 (2008), p. 166-174. -
További szerzők:	Somogyi Ildikó Kiszler Gábor Németh József (1954-) (vegyész, analitikus) Reglődi Dóra (Idegtudományok) Lubics Andrea (Pécs) Kiss Péter Tamás Andrea (Idegtudomány) (Pécs) Pollák Edit Molnár László
Internet cím:	elektronikus változat DOI
Borító:
Saját polcon: