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Dátum csökkenő
1.
001-es BibID:
BIBFORM003873
Első szerző:
Boros Ákos
Cím:
Changes in the expression of PACAP-like compounds during the embryonic development of the earthworm eisenia fetida / Akos Boros, Dora Reglodi, Zsofia Herbert, Gabor Kiszler, Jozsef Nemeth, Andrea Lubics, Peter Kiss, Andrea Tamas, Seiji Shioda, Kouhei Matsuda, Edit Pollak, Laszló Molnar
Dátum:
2008
Megjegyzések:
Pituitary adenylate cyclase-activating polypeptide (PACAP) is expressed at very early stages in the vertebrate nervous system, and its functions in the embryonic development have been shown by various studies. PACAP is an extremely conserved molecule in phylogeny; however, little is known about its presence and functions in invertebrates. Our previous studies have shown the occurrence of PACAP-like immunoreactivity in the invertebrate nervous system. The aim of this study was to investigate the presence and localization of PACAP-like compounds during the embryonic development of earthworms from cocoon deposition to hatching using immunological methods (radioimmunoassay, dot blot, immunohistochemistry). PACAP-like immunoreactive compounds were detected at very early stages of the embryonic development of the earthworm Eisenia fetida. No significant changes were observed during the early stages in the developing embryo, but a marked increase occurred before hatching. In contrast, during the embryonic development, the level of PACAP-like compounds gradually decreased in cocoon fluids. Immunohistochemistry revealed the presence of PACAP-like immunoreactive cell bodies and processes in the developing body wall, prostomium, pharyngeal wall, and central nervous system. Cells located in the body wall correspond to putative progenitor cells of primary sensory cells. In the present study, we also showed that the clitellum (reproductive organ) of sexually mature worms contained significantly higher levels of PACAP-like immunoreactivity than other regions of the same animals or the clitellar region of a non-reproducing animal. In summary, these observations provide a morphological basis and suggest a role of PACAP(-like peptides) in the reproductive and developmental functions of invertebrates.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Dot blot
Clitellum
Cocoon
Earthworm
embryo
Primary sensory cells
Radioimmunoassay
Megjelenés:
Journal of molecular neuroscience. - 36 : 1-3 (2008), p. 157-165. -
További szerzők:
Reglődi Dóra (Idegtudományok)
Herbert Zsófia
Kiszler Gábor
Németh József (1954-) (vegyész, analitikus)
Lubics Andrea (Pécs)
Kiss Péter
Tamás Andrea (Idegtudomány) (Pécs)
Shioda, Seiji
Matsuda, Kouhei
Pollák Edit
Molnár László
Internet cím:
elektronikus változat
DOI
Borító:
Saját polcon:
2.
001-es BibID:
BIBFORM003867
Első szerző:
Hernádi László
Cím:
The presence and distribution of pituitary adenylate cyclase activating polypeptide and its receptor in the snail helix pomatia / L. Hernádi, Z. Pirger, T. Kiss, J. Németh, L. Mark, P. Kiss, A. Tamas, A. Lubics, G. Toth, S. Shioda, D. Reglodi
Dátum:
2008
Megjegyzések:
The aim of this study was to show the presence, distribution and function of the pituitary adenylate cyclase activating polypeptide (PACAP) and its receptors in the CNS and peripheral nervous system of the mollusk, Helix pomatia. PACAP-like and pituitary adenylate cyclase activating polypeptide receptor (PAC1-R)-like immunoreactivity was abundant both in the CNS and the peripheral nervous system of the snail. In addition several non-neuronal cells also revealed PACAP-like immunoreactivity. In inactive animals labeled cell bodies were mainly found and in the neuropile of active animals dense immunostained fiber system was additionally detected suggesting that expression of PACAP-like peptide was affected by the behavioral state of the animal. RIA measurements revealed the existence of both forms of PACAP in the CNS where the 27 amino acid form was found to be dominant. The concentration of PACAP27 was significantly higher in samples from active animals supporting the data obtained by immunohistochemistry. In Western blot experiments PACAP27 and PACAP38 antibodies specifically labeled protein band at 4.5 kDa both in rat and snail brain homogenates, and additionally an 14 kDa band in snail. The 4.5 kDa protein corresponds to PACAP38 and the 14 kDa protein corresponds to the preproPACAP or to a PACAP-like peptide having larger molecular weight than mammalian PACAP38. In matrix-assisted laser desorption ionization time of flight (MALDI TOF) measurements fragments of PACAP38 were identified in brain samples suggesting the presence of a large molecular weight peptide in the snail. Applying antibodies developed against the PACAP receptor PAC1-R, immunopositive stained neurons and a dense network of fibers were identified in each of the ganglia. In electrophysiological experiments, extracellular application of PACAP27 and PACAP38 transiently depolarized or increased postsynaptic activity of neurons expressing PAC1-R. In several neurons PACAP elicited a long lasting hyperpolarization which was eliminated after 1.5 h continuous washing. Taken together, these results indicate that PACAP may have significant role in a wide range of basic physiological functions in snail.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
pituitary adenylate cyclase activating polypeptide,
Megjelenés:
Neuroscience. - 155 : 2 (2008), p. 387-402. -
További szerzők:
Pirger Zsolt
Kiss Tibor
Németh József (1954-) (vegyész, analitikus)
Márk László (1956-) (belgyógyász, kardiológus)
Kiss Péter
Tamás Andrea (Idegtudomány) (Pécs)
Lubics Andrea (Pécs)
Tóth Gábor
Shioda, Seiji
Reglődi Dóra (Idegtudományok)
Internet cím:
elektronikus változat
DOI
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