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001-es BibID:	BIBFORM008849
Első szerző:	Börzsei Rita
Cím:	Presence of pituitary adenylate cyclase activating polypeptide-38 in human plasma and milk / Rita Borzsei, Laszlo Mark, Andrea Tamas, Terez Bagoly, Csaba Bay, Katalin Csanaky, Eszter Banki, Peter Kiss, Alexandra Vaczy, Gabriella Horvath, Jozsef Nemeth, Edit Szauer, Zsuzsanna Helyes, Dora Reglodi
Dátum:	2009
ISSN:	0804-4643 (Print)
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide widely distributed throughout the body. It is involved in the regulation of various physiological and pathophysiological processes, such as reproduction, thermoregulation, motor activity, brain development, neuronal survival, inflammation and pain. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma. Furthermore, based on the presence of vasoactive intestinal peptide, structurally the closest to PACAP, in milk and PACAP and its receptors in the mammary gland, our aim was to study PACAP-38 in human milk.Design and methods: The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20-40), as well as in plasma and milk samples from lactating women (age: 20-35). PACAP concentration was measured with a specific and sensitive RIA. Results: Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable in healthy volunteers and it is not significantly altered by gender, age, food intake or hormonal cycle in females. However, PACAP-38 plasma levels significantly increased in lactating women having 1-6 month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in the human milk with levels 5-20-fold greater in the milk whey than in the respective plasma samples. Conclusions: We found PACAP-38 in human plasma and its increase during the first 6 months of the lactation period. A prominent, nearly 10-fold higher concentration of this peptide was detected in human milk. Based on the literature, several important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer as well as regulation of growth/differentiation of certain tissues of the neonates. The novelty of the present descriptive data provides a basis for further investigations on the mechanism of PACAP-38 secretion in human milk and its functional significance.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban PACAP-38 human plasma, human milk
Megjelenés:	European Journal of Endocrinology. - 160 : 4 (2009), p. 561-565. -
További szerzők:	Márk László (1956-) (belgyógyász, kardiológus) Tamás Andrea (Idegtudomány) (Pécs) Bagoly Teréz Bay Csaba Csanaky Katalin Bánki Eszter Kiss Péter Váczy Alexandra Horváth Gabriella Németh József (1954-) (vegyész, analitikus) Szauer Edit Helyes Zsuzsanna Reglődi Dóra (Idegtudományok)
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001-es BibID:	BIBFORM021890
Első szerző:	Czeglédi Levente (agrármérnök)
Cím:	Presence of pituitary adenylate cyclase-activating polypeptide (PACAP) in the plasma and milk of ruminant animals / Czeglédi Levente, Tamás Andrea, Borzsei Rita, Bagoly Teréz, Kiss Péter, Horváth Gabriella, Brubel Réka, Németh József, Szalontai Bálint, Szabadfi Krisztina, Jávor András, Reglódi Dóra, Helyes Zsuzsanna
Dátum:	2011
Megjegyzések:	Milk contains a variety of proteins and peptides that possess biological activity. Growth factors, such as growth hormone, insulin-like, epidermal and nerve growth factors are important milk components which may regulate growth and differentiation in various neonatal tissues and also those of the mammary gland itself. We have recently shown that pituitary adenylate cyclase-activating polypeptide (PACAP), an important neuropeptide with neurotrophic actions, is present in the human milk in much higher concentration than in the plasma of lactating women. Investigation of growth factors in the milk of domestic animals is of utmost importance for their nutritional values and agricultural significance. Therefore, the aim of the present study was to determine the presence and concentration of PACAP in the plasma and milk of three ruminant animal species. Furthermore, the presence of PACAP and its specific PAC1 receptor were investigated in the mammary glands. Radioimmunoassay measurements revealed that PACAP was present in the plasma and the milk of the sheep, goat and the cow in a similar concentration to that measured previously in humans. PACAP38-like immunoreactivity (PACAP38-LI) was 5-20-fold higher in the milk than in the plasma samples of the respective animals, a similar serum/milk ratio was found in all the three species. The levels did not show significant changes within the examined 3-month-period of lactation after delivery. Similar PACAP38-LI was measured in the homogenates of the sheep mammary gland samples taken 7 and 30 days after delivery. PAC1 receptor expression was detected in these udder biopsies by fluorescent immunohistochemistry suggesting that this peptide might have an effect on the mammary glands themselves. These data show that PACAP is present in the milk of various ruminant domestic animal species at high concentrations, the physiological implications of which awaits further investigation.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	General and Comparative Endocrinology. - 172 : 1 (2011), p. 115-119. -
További szerzők:	Tamás Andrea (Idegtudomány) (Pécs) Borzsei Rita (agrár) Bagoly Teréz Kiss Péter Horváth Gabriella Brubel Réka Németh József (1954-) (vegyész, analitikus) Szalontai Bálint (Pécsi Egyetem) Szabadfi Krisztina (Pécsi Egyetem) Jávor András (1952-) (agrármérnök) Reglődi Dóra (Idegtudományok) Helyes Zsuzsanna
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM013279
Első szerző:	Horváth Gabriella
Cím:	Mice deficient in pituitary adenylate cyclase activating polypeptide display increased sensitivity to renal oxidative stress in vitro / Gabriella Horvath, Laszlo Mark, Reka Brubel, Peter Szakaly, Boglarka Racz, Peter Kiss, Andrea Tamas, Zsuzsanna Helyes, Andrea Lubics, Hitoshi Hashimoto, Akemichi Baba, Norihito Shintani, Gergely Furjes, Jozsef Nemeth, Dora Reglodi
Dátum:	2010
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional neuropeptide, showingwidespread occurrence in the nervous system and also in peripheral organs. The neuroprotective effectsof PACAP are well-established in different neuronal systems against noxious stimuli in vitro and in vivo.Recently, its general cytoprotective actions have been recognized, including renoprotective effects. However,the effect of endogenous PACAP in the kidneys is not known. The main aim of the present studywas to investigate whether the lack of this endogenous neuropeptide influences survival of kidney cellsagainst oxidative stress. First, we determined the presence of endogenous PACAP from mouse kidneyhomogenates by mass spectrometry and PACAP-like immunoreactivity by radioimmunoassay. Second,primary cultures were isolated from wild type and PACAP deficient mice and cell viability was assessedfollowing oxidative stress induced by 0.5, 1.5 and 3mM H2O2. Our mass spectrometry and radioimmunoassayresults show that PACAP is endogenously present in the kidney. The main part of our studyrevealed that the sensitivity of cells from PACAP deficient mice was increased to oxidative stress: bothafter 2 or 4 h of exposure, cell viability was significantly reduced compared to that from control wild typemice. This increased sensitivity of kidneys from PACAP deficient mice could be counteracted by exogenouslygiven PACAP38. These results show, for the first time, that endogenous PACAP protects againstoxidative stress in the kidney, and that PACAP may act as a stress sensor in renal cells. These findingsfurther support the general cytoprotective nature of this neuropeptide.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Cell viability Oxidative stress PACAP knockout mice Renoprotection
Megjelenés:	Neuroscience Letters. - 469 : 1 (2010), p. 70-74. -
További szerzők:	Márk László (1956-) (belgyógyász, kardiológus) Brubel Réka Szakály Péter Rácz Boglárka Kiss Péter Tamás Andrea (Idegtudomány) (Pécs) Helyes Zsuzsanna Lubics Andrea (Pécs) Hashimoto, Hitoshi Baba, Akemichi Shintani Norihito Fürjes Gergely Németh József (1954-) (vegyész, analitikus) Reglődi Dóra (Idegtudományok)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM087705
Első szerző:	Kiss Péter (Pécs)
Cím:	Presence of PACAP-38 in mammalian plasma and milk : from guinea pig to humans / Kiss P., Csanaky K., Bánki E., Tamás A., Börzsei R., Márk L., Bagoly T., Bay Cs., Váczy A., Horváth G., Németh J., Czeglédi L., Szauer E., Helyes Zs., Reglődi D.
Dátum:	2010
ISSN:	0231-424X 1588-2683
Megjegyzések:	PACAP is a pleiotropic and multifunctional neuropeptide widely distributed throughout the body. It is involved in the regulation of various physiological and pathophysiological processes, such as reproduction, thermoregulation, motor activity, brain development, neuronal survival, inflammation and pain. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma. Furthermore, based on the presence of VIP, structurally the closest to PACAP, in milk, and presence of PACAP- and PACAP receptor-immunoreactive elements in the mammary gland, our aim was also to examine PACAP-38 in human milk. For comparative studies, we investigated presence of PACAP in plasma and milk of other species, including goat, sheep, cow and guinea pig. Methods. The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20?40), in plasma and milk samples from lactating women (age: 20?35). PACAP concentration was measured with a specific and sensitive radioimmunoassay in human and other mammalian samples. Results. Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable in healthy volunteers and it is not significantly altered by gender, age, food intake or the hormonal cycle in females. However, PACAP-38 plasma levels significantly increased in lactating women having 1?6-month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in the human milk with levels 5?20-fold greater in the milk whey than in the respective plasma samples. The same ratio was found in all other examined species. Conclusions. Based on the present findings and the literature, several important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer as well as regulation of growth/differentiation of certain tissues of the neonates.
Tárgyszavak:	Orvostudományok Egészségtudományok idézhető absztrakt folyóiratcikk PACAP-38
Megjelenés:	Acta Physiologica Hungarica. - 97 : 1 (2010), p. 115. -
További szerzők:	Csanaky Katalin Bánki Eszter Tamás Andrea (Idegtudomány) (Pécs) Börzsei Rita Márk László Bagoly Teréz Bay Csaba Váczy Alexandra Horváth Gabriella Németh József (1954-) (vegyész, analitikus) Czeglédi Levente (1977-) (agrármérnök) Szauer Edit Helyes Zsuzsanna Reglődi Dóra (Idegtudományok)
Pályázati támogatás:	K72592 OTKA K73044 OTKA F67830 OTKA 78480 OTKA K75965 OTKA Gedeon Richter Centenary Foundation Egyéb Janos Bolyai Postdoctoral Research Fellowship Egyéb
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