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1.

001-es BibID:BIBFORM031291
Első szerző:Brubel Réka
Cím:Presence of pituitary adenylate cyclase activating polypeptide and its type I receptor in the rat kidney / Brubel R., Horvath G., Reglodi D., Lubics A., Tamas A., Kiss P., Laszlo E., Nemeth J., Mark L., Szakaly P.
Dátum:2011
Megjegyzések:Pituitary adenylate cyclase activating polypeptide (PACAP), a multifunctional neuropeptide,has 2 active forms, PACAP38 and PACAP27. It is now well-established that PACAPhas several actions also in peripheral organs, including renoprotective effects. The peptideitself has not been previously identified in the rat kidney. The first aim of our study was toidentify PACAP in the rat kidney using mass spectrometry and radioimmunoassay (RIA).Receptor mRNA and binding studies revealed the existence of all 3 PACAP receptors(PAC1, VPAC1, and VPAC2) in the kidney, but their exact localization in histologicsections was not evident. Because most of the cytoprotective effects of PACAP relate to itsspecific PAC1 receptor, our second aim was to identify the cell types wherein the PAC1receptor is expressed in the rat kidney. Mass spectrometry revealed the presence ofPACAP38 in the kidney. RIA measurements showed both PACAP38- and PACAP27-likeimmunoreactivities in kidney homogenates, with PACAP38 being dominant. Immunohistochemistryrevealed PAC1 receptor-like immunoreactivity in kidney sections, mainlyexpressed in cortical tubular epithelial cells. These results showed PACAP to beendogenously present in the kidney. The tubular localization of the PAC1 receptorprovides the basis for the renal effects of the peptide under physiologic and pathologicconditions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
PACAP38
PACAP27
rat kidney
receptor
Megjelenés:Transplantation Proceedings. - 43 : 4 (2011), p. 1297-1299. -
További szerzők:Horváth Gábor (Pécs) Reglődi Dóra (Idegtudományok) Lubics Andrea (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Kiss Péter (Pécs) László E. Németh József (1954-) (vegyész, analitikus) Mark L. Szakály Péter
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2.

001-es BibID:BIBFORM013274
Első szerző:Brubel Réka
Cím:Changes in the Expression of Pituitary Adenylate Cyclase-Activating Polypeptide in the Human Placenta during Pregnancy and Its Effects on the Survival of JAR Choriocarcinoma Cells/ R. Brubel, A. Boronkai, D. Reglodi, B. Racz, J. Nemeth, P. Kiss, A. Lubics, G. Toth, G. Horvath, T. Varga, D. Szogyi, E. Fonagy, J. Farkas, A. Barakonyi, Sz. Bellyei, L. Szereday, M. Koppan, A. Tamas
Dátum:2010
Megjegyzések:Pituitary adenylate cyclase-activating polypeptide(PACAP), a neuropeptide with survival-promotingactions, has been observed in endocrine organs and isthought to play a role in reproductive functions, includingpregnancy. PACAP occurs in two forms, 27 and 38 aminoacid residues, with PACAP38 being the predominant formin human tissues. In the present study, we determined theconcentrations of PACAP38 and PACAP27 in firsttrimesterand full-term human placentas using radioimmunoassay.We found high levels of PACAP38 and lowerlevels of PACAP27 in different parts of the full-term humanplacenta. PACAP38 content increased in the placentaduring pregnancy, both on the maternal side and on thefetal side. The effects of PACAP on the survival of JARhuman choriocarcinoma cells were investigated using flowcytometry and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cell viability assay in cellsexposed to the widely used chemotherapeutic agentmethotrexate (MTX). It was found that PACAP neitherinfluenced the survival of JAR cytotrophoblast cells noraffected cellular response to the death-inducing effect of thechemotherapeutic agent MTX. The present observationsfurther support the significance of PACAP in the humanplacenta. The observation that PACAP did not influence theeffects of MTX may have future clinical importance,showing that PACAP does not decrease the effects ofcertain chemotherapeutic agents.Keywords JAR . Choriocarcinoma . RIA .
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
JAR
Choriocarcinoma
RIA
PACAP38
Megjelenés:Journal of Molecular Neuroscience. - 42 : 3 (2010), p. 450-458. -
További szerzők:Boronkai A. Reglődi Dóra (Idegtudományok) Rácz B. (Pécs) Németh József (1954-) (vegyész, analitikus) Kiss Péter (Pécs) Lubics Andrea (Pécs) Tóth Gábor (Szeged) Horváth Gábor (Pécs) Varga T. (Pécs) Szogyi D. (Pécs) Fonagy E. Farkas J. (Pécs) Barakonyi A. Bellyei Szabolcs Szereday László Koppán Miklós Tamás A. (Pécs)
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3.

001-es BibID:BIBFORM087705
Első szerző:Kiss Péter (Pécs)
Cím:Presence of PACAP-38 in mammalian plasma and milk : from guinea pig to humans / Kiss P., Csanaky K., Bánki E., Tamás A., Börzsei R., Márk L., Bagoly T., Bay Cs., Váczy A., Horváth G., Németh J., Czeglédi L., Szauer E., Helyes Zs., Reglődi D.
Dátum:2010
ISSN:0231-424X 1588-2683
Megjegyzések:PACAP is a pleiotropic and multifunctional neuropeptide widely distributed throughout the body. It is involved in the regulation of various physiological and pathophysiological processes, such as reproduction, thermoregulation, motor activity, brain development, neuronal survival, inflammation and pain. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma. Furthermore, based on the presence of VIP, structurally the closest to PACAP, in milk, and presence of PACAP- and PACAP receptor-immunoreactive elements in the mammary gland, our aim was also to examine PACAP-38 in human milk. For comparative studies, we investigated presence of PACAP in plasma and milk of other species, including goat, sheep, cow and guinea pig. Methods. The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20?40), in plasma and milk samples from lactating women (age: 20?35). PACAP concentration was measured with a specific and sensitive radioimmunoassay in human and other mammalian samples. Results. Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable in healthy volunteers and it is not significantly altered by gender, age, food intake or the hormonal cycle in females. However, PACAP-38 plasma levels significantly increased in lactating women having 1?6-month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in the human milk with levels 5?20-fold greater in the milk whey than in the respective plasma samples. The same ratio was found in all other examined species. Conclusions. Based on the present findings and the literature, several important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer as well as regulation of growth/differentiation of certain tissues of the neonates.
Tárgyszavak:Orvostudományok Egészségtudományok idézhető absztrakt
folyóiratcikk
PACAP-38
Megjelenés:Acta Physiologica Hungarica. - 97 : 1 (2010), p. 115. -
További szerzők:Csanaky Katalin Bánki Eszter Tamás Andrea (Idegtudomány) (Pécs) Börzsei Rita Márk László Bagoly Teréz Bay Csaba Váczy Alexandra Horváth Gabriella Németh József (1954-) (vegyész, analitikus) Czeglédi Levente (1977-) (agrármérnök) Szauer Edit Helyes Zsuzsanna Reglődi Dóra (Idegtudományok)
Pályázati támogatás:K72592
OTKA
K73044
OTKA
F67830
OTKA
78480
OTKA
K75965
OTKA
Gedeon Richter Centenary Foundation
Egyéb
Janos Bolyai Postdoctoral Research Fellowship
Egyéb
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4.

001-es BibID:BIBFORM013278
Első szerző:Reglődi Dóra (Idegtudományok)
Cím:Alterations of pituitary adenylate cyclase-activating polypeptide-like immunoreactivity in the human plasma during pregnancy and after birth / D. Reglodi, J. Gyarmati, T. Ertl, R. Borzsei, J. Bodis, A. Tamas, P. Kiss, K. Csanaky, E. Banki, C. Bay, J. Nemeth, Z. Helyes
Dátum:2010
Megjegyzések:Pituitary adenylate cyclase-activating polypeptide (PACAP)was discovered from the hypothalamus and it belongs tothe vasoactive intestinal peptide (VIP)/secretin/glucagonspeptide family (1). It occurs in two biologically active forms,with 38 and 27 amino-acid residues. The first studies reportedon its adenylate cyclase activation in pituitary cellsand described its distribution in hypothalamic nuclei influencingpituitary hormone secretion (1). Although the last 20years since its discovery have revealed numerous distincteffects in the nervous system and various peripheral organs,its endocrine functions still represent a major focusin PACAP research. PACAP influences, among others, therelease of GnRH and FSH in different species (2, 3) andthe secretion of PRL (4-6). PACAP also influences sexualbehavior, ovarian follicular maturation, and fertility (3, 7,8), uteroplacental circulation (9), and trophoblast survival(10). Furthermore, PACAP is a very important trophic factor,especially during the development of the nervous system(1, 11). Radioimmunoassay (RIA) analysis revealed thatPACAP38 concentration is higher in the hypophyseal portalblood than in the peripheral circulation in the rat (12). Inspite of the vast literature on PACAP, there are only fewhuman studies. The presence of PACAP has been shownin human blood and some other tissues (1, 13). The half-lifeof PACAP in the circulation is relatively short, only a fewminutes, due to rapid enzymatic degradation by dipeptidyl-peptidase IV (14). We have recently shown the presenceof PACAP38 in human milk by mass spectrometry,and we have also found that PACAP38-like immunoreactivity(PACAP38-LI) is 5-20 times higher in the milk than inthe plasma (15). In humans, PACAP has also been shownto influence PRL, vasopressin, and oxytocin secretion (5,16). Immunohistochemical studies have revealed the presenceof PACAP and its receptors in the female reproductivesystem, including the placenta, uterus, and mammarygland (9, 17). Recent studies have suggested the involvementof PACAP in the pathophysiological processes ofstress urinary incontinence and the prolapse of pelvic organsin female patients (18).Regarding the role of PACAP in reproductive functionsand the urging need to obtain further human data, theaim of the present study was to investigate whether theconcentration of PACAP is altered during normal pregnancy,delivery, and after childbirth. We also measuredthe levels of endogenous PACAP with RIA in the peripheralblood and of newborn babies, as well as in the bloodobtained from the umbilical arteries and veins.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Delivery
newborn
PACAP
RIA
umbilical vessels
Megjelenés:Journal of Endocrinological Investigation. - 33 : 7 (2010), p. 443-445. -
További szerzők:Gyarmati János (1926-1983) (radiológus) Ertl Tibor Börzsei Rita Bodis József (Pécs) Tamás A. (Pécs) Kiss Péter (Pécs) Csanaky Katalin Bánki Eszter Bay Csaba Németh József (1954-) (vegyész, analitikus) Helyes Zsuzsanna
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5.

001-es BibID:BIBFORM040248
035-os BibID:PMID:16888211
Első szerző:Szakály Péter
Cím:Changes in PACAP levels in the central nervous system after ovariectomy and castration / P. Szakaly, G. Horvath, P. Kiss, E. Laszlo, J. Farkas, G. Furjes, J. Nemeth, D. Reglodi
Dátum:2006
ISSN:0077-8923
Megjegyzések:The aim of the present article was to investigate the influenceof gonadectomy on pituitary adenylate cyclase-activating polypeptide(PACAP) levels in different brain areas. In males, there seems tobe an inverse relationship between gonadotropins and PACAP in thebrain in the acute phase of castration: PACAP levels decreased in almostall brain areas examined within the first week after castration. Infemales, such pattern was observed in the hypothalamus, brain stem,and temporal cortex. In the pituitary, levels decreased only on the firstday after ovariectomy, and later, as in the thalamus, increases were observed.Although the pattern of change showed gender differences, ourresults provide further evidence that levels of gonadotropins and possiblygonadotropin-releasing hormone influence PACAP levels and thatPACAP is involved in the regulation of gonadal functions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Annals of the New York Academy of Sciences. - 1070 (2006), p. 468-473. -
További szerzők:Németh József (1954-) (vegyész, analitikus) Horváth Gábor (Pécs) Kiss Péter (Pécs) László E. Farkas J. (Pécs) Fürjes Gergely Reglődi Dóra (Idegtudományok)
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DOI
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6.

001-es BibID:BIBFORM013280
Első szerző:Szakály Péter
Cím:Changes in Pituitary Adenylate Cyclase-Activating Polypeptide Following Renal Ischemia-Reperfusion in Rat / P. Szakaly, G. Horvath, P. Kiss, E. Laszlo, J. Farkas, G. Furjes, J. Nemeth, D. Reglodi
Dátum:2010
Megjegyzések:PACAP (pituitary adenylate cyclase-activating polypeptide) occurs in two biologicallyactive forms, with 38 and 37 amino acid residues (PACAP38 and PACAP27). Inmammalian tissues, PACAP38 is the dominant form. Diverse effects have been describedin the cardiovascular, respiratory, gastrointestinal, and urogenital systems. PACAP isknown for its strong cytoprotective effects, which are present endogenously as well, asproven by knockout studies and results showing that PACAP is up-regulated followingdiverse injuries. Little is known about such effects in the kidney. We have previously shownthat PACAP is protective in renal ischemia-reperfusion injury. Therefore, the aim of thepresent study was to investigate the changes of endogenous PACAP following 60-minuterenal ischemia using radioimmunoassay. Changes were observed within 24 hours followingrenal vessel clamping. In the cortex, an acute decrease was followed by an increase on theintact side, and levels returned to original ones on the operated side. In the medulla,changes were only observed on the clamped side: a marked up-regulation was detected inPACAP38-like immunoreactivity within the first 24 hours. The present study showed thatPACAP38- and PACAP27-like immunoreactivities sensitively react to renal ischemiareperfusion,the physiological importance of which awaits further investigation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
PACAP38 and PACAP27
Megjelenés:Transplantation Proceedings. - 42 : 6 (2010), p. 2283-2286. -
További szerzők:Horváth Gábor (Pécs) Kiss Péter (Pécs) László E. Farkas J. (Pécs) Fürjes Gergely Németh József (1954-) (vegyész, analitikus) Reglődi Dóra (Idegtudományok)
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