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001-es BibID:	BIBFORM031294
Első szerző:	Brubel Réka
Cím:	Investigation of pituitary adenylate cyclase activating polypeptide in human gynecological and other biological fluids by using MALDI TOF mass spectrometry. / Brubel R., Reglodi D., Jambor E., Koppan M., Varnagy A., Biro Z., Kiss P., Gaal V., Bodis J., Bay Cs., Veszpremi B., Tamas A., Nemeth J., Mark L.
Dátum:	2011
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional and pleiotropic neuropeptide. PACAP hasdiverse effects in the endocrine system, among others, it plays important roles in oogenesis, implantation and development ofthe nervous system.However, it is not knownwhether PACAP is present in the fluids of the human reproductive organs. The aimof the present study was to determine, by means of mass spectrometry and radioimmunoassay, whether PACAP is present inhuman amniotic fluid, ovarian follicular fluid and cervico-vaginal fluid. Samples were obtained from healthy adult volunteers.Our MALDI TOF and MALDI TOF/TOF spectrometry results show that PACAP38 is present in all of the follicular fluid samples,and PACAP-like immonoreactivity was also measured by radioimmunoassay. However, we did not find the characteristic peakrepresenting the unmodified 38 amino acid form of the peptide in normal cervico-vaginal smear and amniotic fluid samples.Furthermore, we analyzed other body fluids for comparison, such as human nasal fluid, saliva and aqueous humor. PACAPwas not found in these latter samples. In summary, the present study provides evidence for the presence of PACAP in humanfollicular fluid, suggesting a role in oocyte function, but determination of the exact physiological significance awaits furtherinvestigation.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban cervico-vaginal fluid amniotic fluid follicular fluid nasal fluid saliva aqueous humor mass spectrometry radioimmunoassay
Megjelenés:	Journal of Mass Spectrometry. - 46 : 2 (2011), p. 189-194. -
További szerzők:	Reglődi Dóra (Idegtudományok) Jámbor E. Koppán Miklós Várnagy A. Biró Zsolt (szemész) Kiss P. Gaál V. Bodis József (Pécs) Bay Csaba Veszprémi B. Tamás A. (Pécs) Németh József (1954-) (vegyész, analitikus) Mark L.
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM042639
Első szerző:	Koppán Miklós
Cím:	Correlation Between Oocyte Number and Follicular Fluid Concentration of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) in Women After Superovulation Treatment / M. Koppan, A. Varnagy, D. Reglodi, R. Brubel, J. Nemeth, A. Tamas, L. Mark, J. Bodis
Dátum:	2012
Megjegyzések:	Follicular growth, ovulation, and luteinization areinfluenced by interactions of peptide and steroid hormonesignalingcascades in the ovary. Pituitary adenylate cyclaseactivatingpolypeptide (PACAP) plays an important role in theregulation of several endocrine processes and is present inovarian follicular fluid (FF). However, little is known aboutPACAP in FF with regard to maturation, ovulation, fertilization,and successful pregnancy. The aim of this pilot study wasto investigate whether there is a correlation between PACAPconcentration in FF and ovarian response to superovulationtreatment in infertile women, performed in volunteers (n0132; aged between 20 and 35). After treatment, the numberof harvested oocytes was recorded and PACAP immunoreactivityin FF was measured by radioimmunoassay. All thecorresponding PACAP concentrations were below 290 fmol/ml in cases when the number of harvested oocytes exceeded 14per patient, while in all cases above 290 fmol/ml, the number ofoocytes was below 14. Using these cutoff values, we determinedthree study groups: high-PACAP concentration, highoocytenumber, and low-PACAP concentration?low-oocytenumber groups. Median values of PACAP concentration inthese groups were 411.2, 106.5, and 101.0 fmol/ml, respectively,while themedian values of harvested oocyteswere 5.5, 19.0,and 5.0, respectively. Differences were significant, indicating acorrelation between concentration of PACAP in FF and thenumber of recruited oocytes. Higher concentrations of PACAPin FF might be associated with lower number of developingoocytes, while low concentrations of PACAP might correlatewith a markedly higher number of ova retrieved, thus predictinga higher chance for ovarian hyperstimulation. Our presentstudy is among the first few human clinical studies with directconclusions drawn for possible clinical impact of PACAP.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban RIA Follicular fluid Superovulation treatment Human Neuropeptide
Megjelenés:	Journal of Molecular Neuroscience. - 48 (2012), p. 617-622. -
További szerzők:	Várnagy A. Reglődi Dóra (Idegtudományok) Brubel Réka Németh József (1954-) (vegyész, analitikus) Tamás A. (Pécs) Mark L. Bodis József (Pécs)
Pályázati támogatás:	OTKA-72592 OTKA
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM013278
Első szerző:	Reglődi Dóra (Idegtudományok)
Cím:	Alterations of pituitary adenylate cyclase-activating polypeptide-like immunoreactivity in the human plasma during pregnancy and after birth / D. Reglodi, J. Gyarmati, T. Ertl, R. Borzsei, J. Bodis, A. Tamas, P. Kiss, K. Csanaky, E. Banki, C. Bay, J. Nemeth, Z. Helyes
Dátum:	2010
Megjegyzések:	Pituitary adenylate cyclase-activating polypeptide (PACAP)was discovered from the hypothalamus and it belongs tothe vasoactive intestinal peptide (VIP)/secretin/glucagonspeptide family (1). It occurs in two biologically active forms,with 38 and 27 amino-acid residues. The first studies reportedon its adenylate cyclase activation in pituitary cellsand described its distribution in hypothalamic nuclei influencingpituitary hormone secretion (1). Although the last 20years since its discovery have revealed numerous distincteffects in the nervous system and various peripheral organs,its endocrine functions still represent a major focusin PACAP research. PACAP influences, among others, therelease of GnRH and FSH in different species (2, 3) andthe secretion of PRL (4-6). PACAP also influences sexualbehavior, ovarian follicular maturation, and fertility (3, 7,8), uteroplacental circulation (9), and trophoblast survival(10). Furthermore, PACAP is a very important trophic factor,especially during the development of the nervous system(1, 11). Radioimmunoassay (RIA) analysis revealed thatPACAP38 concentration is higher in the hypophyseal portalblood than in the peripheral circulation in the rat (12). Inspite of the vast literature on PACAP, there are only fewhuman studies. The presence of PACAP has been shownin human blood and some other tissues (1, 13). The half-lifeof PACAP in the circulation is relatively short, only a fewminutes, due to rapid enzymatic degradation by dipeptidyl-peptidase IV (14). We have recently shown the presenceof PACAP38 in human milk by mass spectrometry,and we have also found that PACAP38-like immunoreactivity(PACAP38-LI) is 5-20 times higher in the milk than inthe plasma (15). In humans, PACAP has also been shownto influence PRL, vasopressin, and oxytocin secretion (5,16). Immunohistochemical studies have revealed the presenceof PACAP and its receptors in the female reproductivesystem, including the placenta, uterus, and mammarygland (9, 17). Recent studies have suggested the involvementof PACAP in the pathophysiological processes ofstress urinary incontinence and the prolapse of pelvic organsin female patients (18).Regarding the role of PACAP in reproductive functionsand the urging need to obtain further human data, theaim of the present study was to investigate whether theconcentration of PACAP is altered during normal pregnancy,delivery, and after childbirth. We also measuredthe levels of endogenous PACAP with RIA in the peripheralblood and of newborn babies, as well as in the bloodobtained from the umbilical arteries and veins.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Delivery newborn PACAP RIA umbilical vessels
Megjelenés:	Journal of Endocrinological Investigation. - 33 : 7 (2010), p. 443-445. -
További szerzők:	Gyarmati János (1926-1983) (radiológus) Ertl Tibor Börzsei Rita Bodis József (Pécs) Tamás A. (Pécs) Kiss Péter (Pécs) Csanaky Katalin Bánki Eszter Bay Csaba Németh József (1954-) (vegyész, analitikus) Helyes Zsuzsanna
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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