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001-es BibID:	BIBFORM061062
Első szerző:	Helyes Zsuzsanna
Cím:	Pituitary Adenylate Cyclase-Activating Polypeptide Is Upregulated in Murine Skin Inflammation and Mediates Transient Receptor Potential Vanilloid-1-Induced Neurogenic Edema / Zsuzsanna Helyes, Jozsef Kun, Nora Dobrosi, Katalin Sándor, Jozsef Németh, Aniko Perkecz, Erika Pintér, Krisztina Szabadfi, Balazs Gaszner, Valeria Tékus, Janos Szolcsányi, Martin Steinhoff, Hitoshi Hashimoto, Dora Reglődi, Tamas Bíró
Dátum:	2015
ISSN:	0022-202X
Megjegyzések:	Although pituitary adenylate cyclase-activating polypeptide (PACAP) was described as a key vasoregulator inhuman skin, little is known about its expression in mouse skin. As it is important to investigate PACAP signaling intranslational mouse dermatitis models, we determined its presence, regulation, and role in neurogenic and nonneurogeniccutaneous inflammatory mechanisms. The mRNA of PACAP and its specific receptor PAC1 wasdetected with real-time PCR in several skin regions at comparable levels. PACAP-38-immunoreactivity measuredwith radioimmunoassay was similar in plantar and dorsal paw skin and the ear but significantly smaller in theback skin. PACAP and PAC1 mRNA, as well as PACAP-38 and PAC1 protein expression, significantly increased in theplantar skin after intraplantar administration of capsaicin (50 ?l, 100 ?gml?1), an agonist of the transient receptorpotential vanilloid 1 (TRPV1) receptor, evoking chiefly neurogenic inflammation without inflammatory cellaccumulation. Intraplantar complete Freund's adjuvant (CFA; 50 ?l, 1mgml?1) also increased PACAP/PAC1 mRNAbut not the PACAP peptide. Capsaicin-induced neurogenic paw edema, but not CFA-evoked non-neurogenicswelling, was significantly smaller in PACAP-deficient mice throughout a 24-hour period. To our knowledge, weprovide previously unreported evidence for PACAP and PAC1 expression upregulation during skin inflammation ofdifferent mechanisms and for its pro-inflammatory function in neurogenic edema formation.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban cross-talk sebaceous gland sebocytes skin homeostasis
Megjelenés:	Journal Of Investigative Dermatology 135 : 9 (2015), p. 2209-2218. -
További szerzők:	Kun József Dobrosi Nóra (1981-) (molekuláris biológus) Sándor Katalin Németh József (1954-) (vegyész, analitikus) Perkecz Anikó Pintér Erika Szabadfi Krisztina (Pécsi Egyetem) Gaszner Balázs (Neuroanatómia) Tékus Valéria Szolcsányi János (Pécs) Steinhoff, Martin Hashimoto, Hitoshi Reglődi Dóra (Idegtudományok) Bíró Tamás (1968-) (élettanász)
Pályázati támogatás:	OTKA-104984 OTKA OTKA-PD100706 OTKA OTKA-101761 OTKA OTKA105369 OTKA TÁMOP-4.2.2./A-11/1/KONV-2012-0025 TÁMOP TÁMOP-4.2.4.A/ 2- 11/1-2012-0001 TÁMOP
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001-es BibID:	BIBFORM013279
Első szerző:	Horváth Gabriella
Cím:	Mice deficient in pituitary adenylate cyclase activating polypeptide display increased sensitivity to renal oxidative stress in vitro / Gabriella Horvath, Laszlo Mark, Reka Brubel, Peter Szakaly, Boglarka Racz, Peter Kiss, Andrea Tamas, Zsuzsanna Helyes, Andrea Lubics, Hitoshi Hashimoto, Akemichi Baba, Norihito Shintani, Gergely Furjes, Jozsef Nemeth, Dora Reglodi
Dátum:	2010
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional neuropeptide, showingwidespread occurrence in the nervous system and also in peripheral organs. The neuroprotective effectsof PACAP are well-established in different neuronal systems against noxious stimuli in vitro and in vivo.Recently, its general cytoprotective actions have been recognized, including renoprotective effects. However,the effect of endogenous PACAP in the kidneys is not known. The main aim of the present studywas to investigate whether the lack of this endogenous neuropeptide influences survival of kidney cellsagainst oxidative stress. First, we determined the presence of endogenous PACAP from mouse kidneyhomogenates by mass spectrometry and PACAP-like immunoreactivity by radioimmunoassay. Second,primary cultures were isolated from wild type and PACAP deficient mice and cell viability was assessedfollowing oxidative stress induced by 0.5, 1.5 and 3mM H2O2. Our mass spectrometry and radioimmunoassayresults show that PACAP is endogenously present in the kidney. The main part of our studyrevealed that the sensitivity of cells from PACAP deficient mice was increased to oxidative stress: bothafter 2 or 4 h of exposure, cell viability was significantly reduced compared to that from control wild typemice. This increased sensitivity of kidneys from PACAP deficient mice could be counteracted by exogenouslygiven PACAP38. These results show, for the first time, that endogenous PACAP protects againstoxidative stress in the kidney, and that PACAP may act as a stress sensor in renal cells. These findingsfurther support the general cytoprotective nature of this neuropeptide.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Cell viability Oxidative stress PACAP knockout mice Renoprotection
Megjelenés:	Neuroscience Letters. - 469 : 1 (2010), p. 70-74. -
További szerzők:	Márk László (1956-) (belgyógyász, kardiológus) Brubel Réka Szakály Péter Rácz Boglárka Kiss Péter Tamás Andrea (Idegtudomány) (Pécs) Helyes Zsuzsanna Lubics Andrea (Pécs) Hashimoto, Hitoshi Baba, Akemichi Shintani Norihito Fürjes Gergely Németh József (1954-) (vegyész, analitikus) Reglődi Dóra (Idegtudományok)
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