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001-es BibID:	BIBFORM040213
Első szerző:	Helyes Zsuzsanna
Cím:	Effects of the somatostatin receptor subtype 4 selective agonist J-2156 on sensory neuropeptide release and inflammatory reactions in rodents / Z. Helyes, E. Pintér, J. Németh, K. Sándor, K. Elekes, A. Szabó, G. Pozsgai, D. Keszthelyi, L. Kereskai, M. Engström, S. Wurster, J. Szolcsányi
Dátum:	2006
ISSN:	0007-1188
Megjegyzések:	Substance P (SP) and calcitonin gene-related peptide (CGRP) released from capsaicin-sensitivesensory nerves induce local neurogenic inflammation; somatostatin exerts systemic anti-inflammatory actions presumably viasst4/sst1 receptors. This study investigates the effects of a high affinity, sst4-selective, synthetic agonist, J-2156, on sensoryneuropeptide release in vitro and inflammatory processes in vivo.Experimental approach: Electrically-induced SP, CGRP and somatostatin release from isolated rat tracheae was measuredwith radioimmunoassay. Mustard oil-induced neurogenic inflammation in rat hindpaw skin was determined by Evans blueleakage and in the mouse ear with micrometry. Dextran-, carrageenan- or bradykinin-induced non-neurogenic inflammationwas examined with plethysmometry or Evans blue, respectively. Adjuvant-induced chronic arthritis was assessed byplethysmometry and histological scoring. Granulocyte accumulation was determined with myeloperoxidase assay and IL-1bwith ELISA.Key results: J-2156 (10-2000 nM) diminished electrically-evoked neuropeptide release in a concentration-dependentmanner. EC50 for the inhibition of substance P, CGRP and somatostatin release were 11.6 nM, 14.3nM and 110.7 nM,respectively. J-2156 (1-100 mgkg-1 i.p.) significantly, but not dose-dependently, inhibited neurogenic and non-neurogenicacute inflammatory processes and adjuvant-induced chronic oedema and arthritic changes. Endotoxin-evoked myeloperoxidaseactivity and IL-1b production in the lung, but not IL-1b- or zymosan-induced leukocyte accumulation in the skin weresignificantly diminished by J-2156.Conclusions and implications: J-2156 acting on sst4 receptors inhibits neuropeptide release, vascular components of acuteinflammatory processes, endotoxin-induced granulocyte accumulation and IL-1b synthesis in the lung and synovial andinflammatory cells in chronic arthritis. Therefore it might be a promising lead for the development of novel anti-inflammatorydrugs.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	British Journal Of Pharmacology. - 149 : 4 (2006), p. 405-415. -
További szerzők:	Pintér E. Németh József (1954-) (vegyész, analitikus) Sándor K. Elekes K. Pozsgai Gábor Keszthelyi Dániel Kereskai László Engström, M. Wurster, S. Szolcsányi János (Pécs)
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001-es BibID:	BIBFORM020215
Első szerző:	Helyes Zsuzsanna
Cím:	Short-Term Fasting Differentially Alters / Z. Helyes, E. Pintér, J. Németh, K. Sándor, K. Elekes, Á. Szabó, G. Pozsgai, D. Keszthelyi, L. Kereskai, M. Engström, S. Wurster, J. Szolcsányi
Dátum:	2006
Megjegyzések:	The present article investigated the levels of pituitary adenylatecyclase-activating polypeptide (PACAP) and vasoactive intestinalpolypeptide (VIP) in the brains of rats and chickens 12, 36, and 84 hafter starvation. PACAP levels increased in both species, 12 h after fooddeprivation in rats, and with a 24-h delay in chickens. VIP levels showeda more complex pattern: a gradual increase in the hypothalamus andtelencephalon, and a significant decrease in the brain stem of rats. Inchickens, a decrease was observed in every brain area after 36 h of starvation.These data showthat PACAP and VIP are differentially regulatedand are involved in the regulatory processes under a food-restricted regimen,and are differentially altered in nocturnal and diurnal species.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban starvation radioimmunoassay hypothalamus brain stem
Megjelenés:	Annals of the New York Academy of Sciences. - 1070 (2006), p. 354-358. -
További szerzők:	Pintér E. Németh József (1954-) (vegyész, analitikus) Sándor K. Elekes K. Szabó Á. Pozsgai Gábor Keszthelyi Dániel Kereskai László Engström, M. Wurster, S. Szolcsányi János (Pécs)
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001-es BibID:	BIBFORM040247
Első szerző:	Németh József (vegyész, analitikus)
Cím:	Effect of pituitary adenylate cyclase activating polypeptide-38 on sensory neuropeptide release and neurogenic inflammation in rats and mice / J. Németh, D. Reglödi, G. Pozsgai, Á. Szabó, K. Elekes, E. Pintér, J. Szolcsányi, Z. Helyes
Dátum:	2006
ISSN:	0306-4522
Megjegyzések:	Substance P (SP) and calcitonin gene-relatedpeptide (CGRP), released from capsaicin-sensitive sensorynerves induce local neurogenic inflammation, while somatostatinexerts systemic anti-inflammatory actions. The aimof the present study was to investigate the release of pituitaryadenylate cyclase activating polypeptide-38 (PACAP-38) andits effects on sensory neuropeptide release in vitro and acuteneurogenic ear swelling in vivo.Capsaicin (10-6 M) or electrical field stimulation (EFS; 40V, 0.1 ms, 10 Hz, 120 s; 1200 impulses)-induced release ofPACAP-38, SP, CGRP and somatostatin from isolated rattracheae was measured with radioimmunoassay. Mustardoil?induced neurogenic inflammation in the mouse ear wasdetermined with a micrometer and in the rat hind paw skin bythe Evans Blue leakage technique.Capsaicin and EFS evoked 27% and more than twofoldelevation of PACAP-38 release respectively, compared withthe prestimulated basal values from isolated trachea preparation.Exogenously administered PACAP-38 (20?2000 nM)diminished both capsaicin- and EFS-evoked sensory neuropeptiderelease in a concentration-dependent manner. Themaximal inhibitory effects of PACAP on capsaicin-inducedsubstance P, CGRP and somatostatin release amounted to75.4%, 73.3% and 90.0%, while EFS-evoked release of thesepeptides was 80.03%, 87.7% and 67.7%. In case of capsaicinstimulation the EC50 values for substance P, CGRP and somatostatinwere 82.9 nM, 60.1 nM and 66.9 nM, respectively.When EFS was performed, these corresponding EC50 datawere 92.1 nM, 67.8 nM and 20.9 nM. PACAP-38 (10, 100 and1000 g/kg i.p. in 200 l volume) inhibited neurogenic earswelling in the mouse. Furthermore, 100 g/kg i.p. PACAPalso significantly diminished mustard oil?evoked plasmaprotein extravasation in the rat skin.These results suggest that PACAP-38 is released from thestimulated peripheral terminals of capsaicin-sensitive afferentsand it is able to inhibit the outflow of sensory neuropeptides.Based on this mechanism of action PACAP is also ableto effectively diminish/abolish neurogenic inflammatory responsein vivo after systemic administration.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Neuroscience. - 143 : 1 (2006), p. 223-230. -
További szerzők:	Reglődi Dóra (Idegtudományok) Pozsgai Gábor Szabó Á. Elekes K. Pintér E. Szolcsányi János (Pécs) Helyes Zsuzsanna
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