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001-es BibID:BIBFORM031294
Első szerző:Brubel Réka
Cím:Investigation of pituitary adenylate cyclase activating polypeptide in human gynecological and other biological fluids by using MALDI TOF mass spectrometry. / Brubel R., Reglodi D., Jambor E., Koppan M., Varnagy A., Biro Z., Kiss P., Gaal V., Bodis J., Bay Cs., Veszpremi B., Tamas A., Nemeth J., Mark L.
Dátum:2011
Megjegyzések:Pituitary adenylate cyclase activating polypeptide (PACAP) is a multifunctional and pleiotropic neuropeptide. PACAP hasdiverse effects in the endocrine system, among others, it plays important roles in oogenesis, implantation and development ofthe nervous system.However, it is not knownwhether PACAP is present in the fluids of the human reproductive organs. The aimof the present study was to determine, by means of mass spectrometry and radioimmunoassay, whether PACAP is present inhuman amniotic fluid, ovarian follicular fluid and cervico-vaginal fluid. Samples were obtained from healthy adult volunteers.Our MALDI TOF and MALDI TOF/TOF spectrometry results show that PACAP38 is present in all of the follicular fluid samples,and PACAP-like immonoreactivity was also measured by radioimmunoassay. However, we did not find the characteristic peakrepresenting the unmodified 38 amino acid form of the peptide in normal cervico-vaginal smear and amniotic fluid samples.Furthermore, we analyzed other body fluids for comparison, such as human nasal fluid, saliva and aqueous humor. PACAPwas not found in these latter samples. In summary, the present study provides evidence for the presence of PACAP in humanfollicular fluid, suggesting a role in oocyte function, but determination of the exact physiological significance awaits furtherinvestigation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
cervico-vaginal fluid
amniotic fluid
follicular fluid
nasal fluid
saliva
aqueous humor
mass spectrometry
radioimmunoassay
Megjelenés:Journal of Mass Spectrometry. - 46 : 2 (2011), p. 189-194. -
További szerzők:Reglődi Dóra (Idegtudományok) Jámbor E. Koppán Miklós Várnagy A. Biró Zsolt (szemész) Kiss P. Gaál V. Bodis József (Pécs) Bay Csaba Veszprémi B. Tamás A. (Pécs) Németh József (1954-) (vegyész, analitikus) Mark L.
Internet cím:DOI
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2.

001-es BibID:BIBFORM031291
Első szerző:Brubel Réka
Cím:Presence of pituitary adenylate cyclase activating polypeptide and its type I receptor in the rat kidney / Brubel R., Horvath G., Reglodi D., Lubics A., Tamas A., Kiss P., Laszlo E., Nemeth J., Mark L., Szakaly P.
Dátum:2011
Megjegyzések:Pituitary adenylate cyclase activating polypeptide (PACAP), a multifunctional neuropeptide,has 2 active forms, PACAP38 and PACAP27. It is now well-established that PACAPhas several actions also in peripheral organs, including renoprotective effects. The peptideitself has not been previously identified in the rat kidney. The first aim of our study was toidentify PACAP in the rat kidney using mass spectrometry and radioimmunoassay (RIA).Receptor mRNA and binding studies revealed the existence of all 3 PACAP receptors(PAC1, VPAC1, and VPAC2) in the kidney, but their exact localization in histologicsections was not evident. Because most of the cytoprotective effects of PACAP relate to itsspecific PAC1 receptor, our second aim was to identify the cell types wherein the PAC1receptor is expressed in the rat kidney. Mass spectrometry revealed the presence ofPACAP38 in the kidney. RIA measurements showed both PACAP38- and PACAP27-likeimmunoreactivities in kidney homogenates, with PACAP38 being dominant. Immunohistochemistryrevealed PAC1 receptor-like immunoreactivity in kidney sections, mainlyexpressed in cortical tubular epithelial cells. These results showed PACAP to beendogenously present in the kidney. The tubular localization of the PAC1 receptorprovides the basis for the renal effects of the peptide under physiologic and pathologicconditions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
PACAP38
PACAP27
rat kidney
receptor
Megjelenés:Transplantation Proceedings. - 43 : 4 (2011), p. 1297-1299. -
További szerzők:Horváth Gábor (Pécs) Reglődi Dóra (Idegtudományok) Lubics Andrea (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Kiss Péter (Pécs) László E. Németh József (1954-) (vegyész, analitikus) Mark L. Szakály Péter
Internet cím:DOI
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3.

001-es BibID:BIBFORM042639
Első szerző:Koppán Miklós
Cím:Correlation Between Oocyte Number and Follicular Fluid Concentration of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) in Women After Superovulation Treatment / M. Koppan, A. Varnagy, D. Reglodi, R. Brubel, J. Nemeth, A. Tamas, L. Mark, J. Bodis
Dátum:2012
Megjegyzések:Follicular growth, ovulation, and luteinization areinfluenced by interactions of peptide and steroid hormonesignalingcascades in the ovary. Pituitary adenylate cyclaseactivatingpolypeptide (PACAP) plays an important role in theregulation of several endocrine processes and is present inovarian follicular fluid (FF). However, little is known aboutPACAP in FF with regard to maturation, ovulation, fertilization,and successful pregnancy. The aim of this pilot study wasto investigate whether there is a correlation between PACAPconcentration in FF and ovarian response to superovulationtreatment in infertile women, performed in volunteers (n0132; aged between 20 and 35). After treatment, the numberof harvested oocytes was recorded and PACAP immunoreactivityin FF was measured by radioimmunoassay. All thecorresponding PACAP concentrations were below 290 fmol/ml in cases when the number of harvested oocytes exceeded 14per patient, while in all cases above 290 fmol/ml, the number ofoocytes was below 14. Using these cutoff values, we determinedthree study groups: high-PACAP concentration, highoocytenumber, and low-PACAP concentration?low-oocytenumber groups. Median values of PACAP concentration inthese groups were 411.2, 106.5, and 101.0 fmol/ml, respectively,while themedian values of harvested oocyteswere 5.5, 19.0,and 5.0, respectively. Differences were significant, indicating acorrelation between concentration of PACAP in FF and thenumber of recruited oocytes. Higher concentrations of PACAPin FF might be associated with lower number of developingoocytes, while low concentrations of PACAP might correlatewith a markedly higher number of ova retrieved, thus predictinga higher chance for ovarian hyperstimulation. Our presentstudy is among the first few human clinical studies with directconclusions drawn for possible clinical impact of PACAP.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
RIA
Follicular fluid
Superovulation treatment
Human
Neuropeptide
Megjelenés:Journal of Molecular Neuroscience. - 48 (2012), p. 617-622. -
További szerzők:Várnagy A. Reglődi Dóra (Idegtudományok) Brubel Réka Németh József (1954-) (vegyész, analitikus) Tamás A. (Pécs) Mark L. Bodis József (Pécs)
Pályázati támogatás:OTKA-72592
OTKA
Internet cím:DOI
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