Találati lista | Tudóstér

001-es BibID:	BIBFORM087705
Első szerző:	Kiss Péter (Pécs)
Cím:	Presence of PACAP-38 in mammalian plasma and milk : from guinea pig to humans / Kiss P., Csanaky K., Bánki E., Tamás A., Börzsei R., Márk L., Bagoly T., Bay Cs., Váczy A., Horváth G., Németh J., Czeglédi L., Szauer E., Helyes Zs., Reglődi D.
Dátum:	2010
ISSN:	0231-424X 1588-2683
Megjegyzések:	PACAP is a pleiotropic and multifunctional neuropeptide widely distributed throughout the body. It is involved in the regulation of various physiological and pathophysiological processes, such as reproduction, thermoregulation, motor activity, brain development, neuronal survival, inflammation and pain. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma. Furthermore, based on the presence of VIP, structurally the closest to PACAP, in milk, and presence of PACAP- and PACAP receptor-immunoreactive elements in the mammary gland, our aim was also to examine PACAP-38 in human milk. For comparative studies, we investigated presence of PACAP in plasma and milk of other species, including goat, sheep, cow and guinea pig. Methods. The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20?40), in plasma and milk samples from lactating women (age: 20?35). PACAP concentration was measured with a specific and sensitive radioimmunoassay in human and other mammalian samples. Results. Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable in healthy volunteers and it is not significantly altered by gender, age, food intake or the hormonal cycle in females. However, PACAP-38 plasma levels significantly increased in lactating women having 1?6-month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in the human milk with levels 5?20-fold greater in the milk whey than in the respective plasma samples. The same ratio was found in all other examined species. Conclusions. Based on the present findings and the literature, several important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer as well as regulation of growth/differentiation of certain tissues of the neonates.
Tárgyszavak:	Orvostudományok Egészségtudományok idézhető absztrakt folyóiratcikk PACAP-38
Megjelenés:	Acta Physiologica Hungarica. - 97 : 1 (2010), p. 115. -
További szerzők:	Csanaky Katalin Bánki Eszter Tamás Andrea (Idegtudomány) (Pécs) Börzsei Rita Márk László Bagoly Teréz Bay Csaba Váczy Alexandra Horváth Gabriella Németh József (1954-) (vegyész, analitikus) Czeglédi Levente (1977-) (agrármérnök) Szauer Edit Helyes Zsuzsanna Reglődi Dóra (Idegtudományok)
Pályázati támogatás:	K72592 OTKA K73044 OTKA F67830 OTKA 78480 OTKA K75965 OTKA Gedeon Richter Centenary Foundation Egyéb Janos Bolyai Postdoctoral Research Fellowship Egyéb
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM042638
Első szerző:	Tuka Bernadett
Cím:	Peripheral and central alterations of pituitary adenylate cyclase activating polypeptide-like immunoreactivity in the rat in response to activation of the trigeminovascular system / Bernadett Tuka, Zsuzsanna Helyes, Adrienn Markovics, Teréz Bagoly, József Németh, László Márk, Réka Brubel, Dóra Reglődi, Árpád Párdutz, János Szolcsányi, László Vécsei, János Tajti
Dátum:	2012
ISSN:	0196-9781
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP) is present in the cranial arteries and trigeminalsensory neurons. We therefore examined the alterations in PACAP-like immunoreactivity (PACAP-LI) ina time-dependent manner in two rat models of trigeminovascular system (TS) activation. In one groupchemical stimulation (CS) was performed with i.p. nitroglycerol (NTG), and in the other one the trigeminalganglia (TRG) were subjected to electrical stimulation (ES). The two biologically active forms, PACAP-38and PACAP-27, were determined by means of radioimmunoassay (RIA) and mass spectrometry (MS) inthe plasma, the cerebrospinal fluid (CSF), the trigeminal nucleus caudalis (TNC), the spinal cord (SC) andthe TRG. The tissue concentrations of PACAP-27 were 10 times lower than those of PACAP-38 in the TNCand SC, but about half in the TRG. PACAP-38, but not PACAP-27, was present in the plasma. Neither formcould be identified in the CSF. PACAP-38-LI in the plasma, SC and TRG remained unchanged after CS, butit was increased significantly in the TNC 90 and 180 min after NTG injection. In response to ES of the TRG,the level of PACAP-38 in the plasma and the TNC was significantly elevated 90 and 180 min later, but notin the SC or the TRG. The alterations in the levels of PACAP-27 in the tissue homogenates in responseto both forms of stimulation were identical to those of PACAP-38. The selective increases in both formsof PACAP in the TNC suggest its important role in the central sensitization involved in migraine-likeheadache.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Peptides. - 33 : 2 (2012), p. 307-316. -
További szerzők:	Helyes Zsuzsanna Markovics Adrienn Bagoly Teréz Németh József (1954-) (vegyész, analitikus) Márk László Brubel Réka Reglődi Dóra (Idegtudományok) Párdutz Árpád Szolcsányi János (Pécs) Vécsei László (1954-) (neurológus) Tajti János
Pályázati támogatás:	OTKA-75965 OTKA
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: