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001-es BibID:	BIBFORM077805
Első szerző:	Borsay Beáta Á. (igazságügyi orvosszakértő)
Cím:	Hypophysiotropic Gonadotropin-Releasing Hormone Projections Are Exposed to Dense Plexuses of Kisspeptin, Neurokinin B and Substance P Immunoreactive Fibers in the Human : a Study on Tissues from Postmenopausal Women / Beáta Á. Borsay, Katalin Skrapits, László Herczeg, Philippe Ciofi, Stephen R. Bloom, Mohammad A. Ghatei, Waljit S. Dhillo, Zsolt Liposits, Erik Hrabovszky
Dátum:	2014
ISSN:	0028-3835
Megjegyzések:	Neuronal populations that synthesize kisspeptin (KP), neurokinin B (NKB) and substance P (SP) in the hypothalamic infundibular nucleus of humans are partly overlapping. These cells are important upstream regulators of gonadotropin-releasing hormone (GnRH) neurosecretion. Homologous neurons in laboratory animals are thought to modulate episodic GnRH secretion primarily via influencing KP receptors on the hypophysiotropic fiber projections of GnRH neurons. To explore the structural basis of this putative axo-axonal communication in humans, we analyzed the anatomical relationship of KP-immunoreactive (IR), NKB-IR and SP-IR axon plexuses with hypophysiotropic GnRH fiber projections. Immunohistochemical studies were carried out on histological samples from postmenopausal women. The neuropeptide-IR axons innervated densely the portal capillary network in the postinfundibular eminence. Subsets of the fibers formed descending tracts in the infundibular stalk, some reaching the neurohypophysis. KP-IR, NKB-IR and SP-IR plexuses intermingled, and established occasional contacts, with hypophysiotropic GnRH fibers in the postinfundibular eminence and through their lengthy course while descending within the infundibular stalk. Triple-immunofluorescent studies also revealed considerable overlap between the KP, NKB and SP signals in individual fibers, providing evidence that these peptidergic projections arise from neurons of the mediobasal hypothalamus. These neuroanatomical observations indicate that the hypophysiotropic projections of human GnRH neurons in the postinfundibular eminence and the descending GnRH tract coursing through the infundibular stalk to the neurohypophysis are exposed to neurotransmitters/neuropeptides released by dense KP-IR, NKB-IR and SP-IR fiber plexuses. Localization and characterization of axonal neuropeptide receptors will be required to clarify the putative autocrine and paracrine interactions in these anatomical regions.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Human Hypothalamus Infundibulum Median eminence Pituitary Reproduction Tachykinins
Megjelenés:	Neuroendocrinology. - 100 : 2-3 (2014), p. 141-152. -
További szerzők:	Skrapits Katalin Herczeg László (1954-) (igazságügyi orvosszakértő) Ciofi, Philippe Bloom, Stephen R. Ghatei, Mohammad A. Dhillo, Waljit S. Liposits Zsolt Hrabovszky Erik
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001-es BibID:	BIBFORM077810
035-os BibID:	(PMID)26388735
Első szerző:	Skrapits Katalin
Cím:	Lateral hypothalamic orexin and melanin-concentrating hormone neurons provide direct input to gonadotropin-releasing hormone neurons in the human / Katalin Skrapits, Vivien Kanti, Zsófia Savanyú, Csilla Maurnyi, Ottó Szenci, András Horváth, Beáta Á. Borsay, László Herczeg, Zsolt Liposits, Erik Hrabovszky
Dátum:	2015
ISSN:	1662-5102 1662-5102
Megjegyzések:	Hypophysiotropic projections of gonadotropin-releasing hormone (GnRH)-synthesizing neurons form the final common output way of the hypothalamus in the neuroendocrine control of reproduction. Several peptidergic neuronal systems of the medial hypothalamus innervate human GnRH cells and mediate crucially important hormonal and metabolic signals to the reproductive axis, whereas much less is known about the contribution of the lateral hypothalamic area to the afferent control of human GnRH neurons. Orexin (ORX)- and melanin-concentrating hormone (MCH)-synthesizing neurons of this region have been implicated in diverse behavioral and autonomic processes, including sleep and wakefulness, feeding and other functions. In the present immunohistochemical study, we addressed the anatomical connectivity of these neurons to human GnRH cells in post-mortem hypothalamic samples obtained from autopsies. We found that 38.9 ± 10.3% and 17.7 ± 3.3% of GnRH-immunoreactive (IR) perikarya in the infundibular nucleus of human male subjects received ORX-IR and MCH-IR contacts, respectively. On average, each 1 mm segment of GnRH dendrites received 7.3 ± 1.1 ORX-IR and 3.7 ± 0.5 MCH-IR axo-dendritic appositions. Overall, the axo-dendritic contacts dominated over the axo-somatic contacts and represented 80.5 ± 6.4% of ORX-IR and 76.7 ± 4.6% of MCH-IR inputs to GnRH cells. Based on functional evidence from studies of laboratory animals, the direct axo-somatic and axo-dendritic input from ORX and MCH neurons to the human GnRH neuronal system may convey critical metabolic and other homeostatic signals to the reproducive axis. In this study, we also report the generation and characterization of new antibodies for immunohistochemical detection of GnRH neurons in histological sections.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban human hypothalamus immunohistochemistry melanin-concentrating hormone orexin
Megjelenés:	Frontiers in Cellular Neuroscience. - 9 (2015), p. 1-13. -
További szerzők:	Kanti Vivien Savanyú Zsófia Maurnyi Csilla Szenci Ottó Horváth András Borsay Beáta Á. (1982-) (igazságügyi orvosszakértő) Herczeg László (1954-) (igazságügyi orvosszakértő) Liposits Zsolt Hrabovszky Erik
Pályázati támogatás:	OTKA K83710 OTKA OTKA K112669 OTKA OTKA K100722 OTKA
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001-es BibID:	BIBFORM077808
035-os BibID:	(PMID)25713511
Első szerző:	Skrapits Katalin
Cím:	Neuropeptide co-expression in hypothalamic kisspeptin neurons of laboratory animals and the human / Katalin Skrapits, Beáta Á. Borsay, László Herczeg, Philippe Ciofi, Zsolt Liposits, Erik Hrabovszky
Dátum:	2015
ISSN:	1662-453X
Megjegyzések:	Hypothalamic peptidergic neurons using kisspeptin (KP) and its co-transmitters for communication are critically involved in the regulation of mammalian reproduction and puberty. This article provides an overview of neuropeptides present in KP neurons, with a focus on the human species. Immunohistochemical studies reveal that large subsets of human KP neurons synthesize neurokinin B, as also shown in laboratory animals. In contrast, dynorphin described in KP neurons of rodents and sheep is found rarely in KP cells of human males and postmenopausal females. Similarly, galanin is detectable in mouse, but not human, KP cells, whereas substance P, cocaine- and amphetamine-regulated transcript and proenkephalin-derived opioids are expressed in varying subsets of KP neurons in humans, but not reported in ARC of other species. Human KP neurons do not contain neurotensin, cholecystokinin, proopiomelanocortin-derivatives, agouti-related protein, neuropeptide Y, somatostatin or tyrosine hydroxylase (dopamine). These data identify the possible co-transmitters of human KP cells. Neurochemical properties distinct from those of laboratory species indicate that humans use considerably different neurotransmitter mechanisms to regulate fertility.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban CART dynorphin hypothalamus kisspeptin neurokinin B reproduction substance P
Megjelenés:	Frontiers in Neuroscience. - 9 (2015), p. 1-6. -
További szerzők:	Borsay Beáta Á. (1982-) (igazságügyi orvosszakértő) Herczeg László (1954-) (igazságügyi orvosszakértő) Ciofi, Philippe Liposits Zsolt Hrabovszky Erik
Pályázati támogatás:	OTKA K83710 OTKA OTKA K112669 OTKA OTKA K100722 OTKA
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001-es BibID:	BIBFORM059183
Első szerző:	Skrapits Katalin
Cím:	Colocalization of Cocaine- and Amphetamine-Regulated Transcript with Kisspeptin and Neurokinin B in the Human Infundibular Region / Katalin Skrapits, Beáta Á. Borsay, László Herczeg, Philippe Ciofi, Stephen R. Bloom, Mohammad A. Ghatei, Waljit S. Dhillo, Zsolt Liposits, Erik Hrabovszky
Dátum:	2014
ISSN:	1932-6203
Megjegyzések:	Kisspeptin (KP)- and neurokinin B (NKB)- synthesizing neurons of the hypothalamic arcuate nucleus play a pivotal role in the regulation of pulsatile gonadotropin-releasing hormone (GnRH) secretion. Unlike in rodents and sheep, the homologous KP and NKB neurons in the human infundibular region rarely express dynorphin- but often exhibit Substance P (SP) immunoreactivity, indicating remarkable species differences in the neurochemical phenotype of these neurons. In search for additional neuropeptides in human KP and NKB neurons, we carried out immunofluorescent studies on hypothalamic sections obtained from five postmenopausal women. Colocalization experiments provided evidence for the presence of cocaine- and amphetamine-regulated transcript (CART) in 47.966.6% of KP-immunoreactive (IR) and 30.064.9% of NKB-IR perikarya and in 17.062.3% of KP-IR and 6.262.0% of NKB-IR axon varicosities. All three neuropeptides were present in 33.364.9% of KP-IR and 28.264.6% of NKB-IR somata, respectively, whereas triple-labeling showed lower incidences in KP-IR (14.361.8%) and NKB-IR (5.962.0%) axon varicosities. CART-IR KP and NKB neurons established contacts with other peptidergic cells, including GnRH-IR neurons and also sent projections to the infundibular stalk. KP and NKB fibers with CART often contained SP as well, while being distinct from CART fibers co-containing the orexigenic peptide agouti-related protein. Presence of CART in human, but not rodent, KP and NKB neurons represents a new example of species differences in the neuropeptide repertoire of mediobasal hypothalamic KP and NKB neurons. Target cells, receptor sites and physiological significance of CART in the efferent communication of KP and NKB neurons in primates require clarification.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban neurons axons nerve fibers Neuropeptides Afferent neurons rodents immune serum Immunohistochemistry techniques
Megjelenés:	Plos One. - 9 : 8 (2014), p. e103977-. -
További szerzők:	Borsay Beáta Á. (1982-) (igazságügyi orvosszakértő) Herczeg László (1954-) (igazságügyi orvosszakértő) Ciofi, Philippe Bloom, Stephen R. Ghatei, Mohammad A. Dhillo, Waljit S. Liposits Zsolt Hrabovszky Erik
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