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001-es BibID:	BIBFORM029094
Első szerző:	Antal Miklós (orvos, anatómus)
Cím:	Expression of hyperpolarization-activated and cyclic nucleotide-gated cation channel subunit 2 in axon terminals of peptidergic nociceptive primary sensory neurons in the superficial spinal dorsal horn of rats / Antal, M., Papp, I., Bahaerguli, N., Veress, G., Vereb, G.
Dátum:	2004
Megjegyzések:	Hyperpolarization-activated cyclic nucleotide-gated cation channel proteins (HCN1-4), which are potentially able to modulate membrane excitability, are abundantly expressed by neurons in spinal dorsal root ganglia (DRG). In the present experiment, we investigated whether HCN2 protein is confined exclusively to the perikarya of DRG neurons or is transported from the somata to the central axons of DRG neurons that terminate in the spinal dorsal horn. Using immunohistochemical methods, we have demonstrated that laminae I-IIo of the superficial spinal dorsal horn of the adult rat spinal cord show a strong punctate immunoreactivity for HCN2. Dorsal rhizotomy resulted in a complete loss of immunostaining in the dorsal horn, suggesting that HCN2 is confined to axon terminals of primary afferents. In double labelling immunohistochemical studies, we have also shown that HCN2 widely co-localizes with calcitonin gene-related peptide, but is almost completely segregated from isolectin-B4 binding, indicating that HCN2 is primarily expressed in peptidergic nociceptive primary afferents. The expression of HCN2 in central terminals of peptidergic primary afferents was also verified with electron microscopy. Utilizing the pre-embedding nanogold method, we found that HCN2 is largely confined to axon terminals with dense-core vesicles. Within these terminals, some of the silver grains marking the accurate location of HCN2 molecules were associated with the cell membrane, and others were scattered in the axoplasm. Within the cell membrane, HCN2 was found almost exclusively in extrasynaptic locations. The results suggest that HCN2 may contribute to the modulation of membrane excitability of nociceptive primary afferent terminals in the spinal dorsal horn.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	The European Journal of Neuroscience 19 : 5 (2004), p. 1336-1342. -
További szerzők:	Papp Ildikó (1976-) (biológus) Bahaerguli, Niyazi Veress Gábor (1971-) (neurobiológus) Vereb György (1965-) (biofizikus, orvos)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat elektronikus változat DOI
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001-es BibID:	BIBFORM050040
Első szerző:	Bhattacharya, Anirban
Cím:	Potentiation of inhibitory synaptic transmission by extracellular ATP in rat suprachiasmatic nuclei / Anirban Bhattacharya, Vojtech Vavra, Irena Svobodova, Zdena Bendova, Gyorgy Vereb, Hana Zemkova
Dátum:	2013
Megjegyzések:	The hypothalamic suprachiasmatic nuclei (SCN), the circadian master clock in mammals, releases ATP in a rhythm, but the role of extracellular ATP in the SCN is still unknown. In this study, we examined the expression and function of ATP-gated P2X receptors (P2XRs) in the SCN neurons of slices isolated from the brain of 16- to 20-day-old rats. Quantitative RT-PCR showed that the SCN contains mRNA for P2X 1-7 receptors and several G-protein-coupled P2Y receptors. Among the P2XR subunits, the P2X2 > P2X7 > P2X4 mRNAs were the most abundant. Whole-cell patch-clamp recordings from SCN neurons revealed that extracellular ATP application increased the frequency of spontaneous GABAergic IPSCs without changes in their amplitudes. The effect of ATP appears to be mediated by presynaptic P2X2Rs because ATPgammaS and 2MeS-ATP mimics, while the P2XR antagonist PPADS blocks, the observed enhancement of the frequency of GABA currents. There were significant differences between two SCN regions in that the effect of ATP was higher in the ventrolateral subdivision, which is densely innervated from outside the SCN. Little evidence was found for the presence of P2XR channels in somata of SCN neurons as P2X2R immunoreactivity colocalized with synapsin and ATP-induced current was observed in only 7% of cells. In fura-2 AM-loaded slices, BzATP as well as ADP stimulated intracellular Ca(2+) increase, indicating that the SCN cells express functional P2X7 and P2Y receptors. Our data suggest that ATP activates presynaptic P2X2Rs to regulate inhibitory synaptic transmission within the SCN and that this effect varies between regions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Adenosine Adenosine Triphosphate Animals Animals,Newborn article Biophysical Phenomena Brain Calcium Cells Cells,Cultured cytology Dose-Response Relationship,Drug drug effects Excitatory Amino Acid Antagonists EXPRESSION gamma-Aminobutyric Acid Gene Expression Regulation genetics In Vitro Male metabolism Neural Inhibition Neurons Patch-Clamp Techniques pharmacology physiology Platelet Aggregation Platelet Aggregation Inhibitors Purinergic Agents Rats Rats,Wistar Receptors,Purinergic P2X Research Research Support rna RNA,Messenger Sodium Sodium Channel Blockers Support Suprachiasmatic Nucleus Synaptic Potentials Synaptic Transmission Tetrodotoxin
Megjelenés:	The Journal of Neuroscience. - 33 : 18 (2013), p. 8035-8044. -
További szerzők:	Vavra, Vojtech Svobodova, Irena Bendova, Zdena Vereb György (1965-) (biofizikus, orvos) Zemkova, Hana
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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