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1.

001-es BibID:BIBFORM006044
Első szerző:Matkó János (biológus)
Cím:Biphasic effect of extracellular ATP on the membrane potential of mouse thymocytes / Matko J., Nagy P., Panyi G., Vereb G. Jr., Bene L., Matyus L., Damjanovich S.
Dátum:1993
Megjegyzések:Extracellular ATP induced changes in the membrane potential of thymocytes from BALB/c mice were analyzed. At concentrations below 0.1 mM, ATP hyperpolarizes the cell membrane on the time scale of development of the Ca(2+)-signal. After a longer time hyperpolarization turns to depolarization. ATP concentrations higher than 0.5 mM caused rapid depolarization without previous hyperpolarization. Verapamil, quinine or the absence of extracellular Ca2+ blocked the hyperpolarization by ATP. In Na(+)-free medium the magnitude of depolarization decreased. Our data suggest a contribution of Ca(2+)-activated K+ channels to the hyperpolarizing effect of ATP at lower concentrations. The direction of membrane potential changes is determined presumably by a sensitive balance of ATP-receptor mediated Ca(2+)- and Na(+)-influx and the Ca(2+)-activated K(+)-channel activity.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Adenosine Triphosphate
Animal
Biophysics
Cell Membrane
cytology
drug effects
Hungary
Kinetics
Membrane Potentials
Mice
Mice,Inbred BALB C
pharmacology
physiology
Quinine
Support,Non-U.S.Gov't
Thymus Gland
Verapamil
Megjelenés:Biochemical and Biophysical Research Communications. - 191 : 2 (1993), p. 378-384. -
További szerzők:Nagy Péter (1971-) (biofizikus) Panyi György (1966-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Bene László (1963-) (biofizikus) Mátyus László (1956-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
Intézményi repozitóriumban (DEA) tárolt változa
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2.

001-es BibID:BIBFORM059574
Első szerző:Nagy Péter (biofizikus)
Cím:Measuring FRET in flow cytometry and microscopy / Péter Nagy, György Vereb, Sándor Damjanovich, László Mátyus, János Szöllősi
Dátum:2006
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Flow Cytometry
Microscopy
Megjelenés:Current Protocols in Cytometry. - p. 1-13.
További szerzők:Vereb György (1965-) (biofizikus, orvos) Damjanovich Sándor (1936-2017) (biofizikus) Mátyus László (1956-) (biofizikus) Szöllősi János (1953-) (biofizikus)
Pályázati támogatás:OTKA-F049025
OTKA
OTKA-T043509
OTKA
OTKA-T043061
OTKA
OTKA-T037831
OTKA
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM004886
Első szerző:Szentesi Gergely (kémia-fizika tanár)
Cím:Computer program for analyzing donor photobleaching FRET image series / Szentesi, G., Vereb, G., Horvath, G., Bodnar, A., Fabian, A., Matko, J., Gaspar, R., Damjanovich, S., Matyus, L., Jenei, A.
Dátum:2005
ISSN:1552-4922
Megjegyzések:The photobleaching fluorescence resonance energy transfer (pbFRET) technique is a spectroscopic method to measure proximity relations between fluorescently labeled macromolecules using digital imaging microscopy. To calculate the energy transfer values one has to determine the bleaching time constants in pixel-by-pixel fashion from the image series recorded on the donor-only and donor and acceptor double-labeled samples. Because of the large number of pixels and the time-consuming calculations, this procedure should be assisted by powerful image data processing software. There is no commercially available software that is able to fulfill these requirements. METHODS: New evaluation software was developed to analyze pbFRET data for Windows platform in National Instrument LabVIEW 6.1. This development environment contains a mathematical virtual instrument package, in which the Levenberg-Marquardt routine is also included. As a reference experiment, FRET efficiency between the two chains (beta2-microglobulin and heavy chain) of major histocompatibility complex (MHC) class I glycoproteins and FRET between MHC I and MHC II molecules were determined in the plasma membrane of JY, human B lymphoma cells. RESULTS: The bleaching time constants calculated on pixel-by-pixel basis can be displayed as a color-coded map or as a histogram from raw image format. CONCLUSION: In this report we introduce a new version of pbFRET analysis and data processing software that is able to generate a full analysis pattern of donor photobleaching image series under various conditions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Algorithms
analysis
beta 2-Microglobulin
Biophysics
Cell Line,Tumor
Cells
Energy Transfer
Fluorescence
Fluorescence Resonance Energy Transfer
Glycoproteins
Histocompatibility Antigens
Human
Humans
Hungary
Lymphoma
Major Histocompatibility Complex
metabolism
methods
Microscopy
Photobleaching
Research
Software
Support
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cytometry. Part A. - 67 : 2 (2005), p. 119-128. -
További szerzők:Vereb György (1965-) (biofizikus, orvos) Horváth Gábor (1974-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Fábián Ákos István (1982-) (aneszteziológus) Matkó János (1952-) (biológus) Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Mátyus László (1956-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

4.

001-es BibID:BIBFORM073622
Első szerző:Szöllősi János (biofizikus)
Cím:Principles of resonance energy transfer / Szöllősi János, Damjanovich Sándor, Nagy Péter, Vereb György, Mátyus László
Dátum:2006
ISBN:Unit1 12.
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
energy transfer
Megjelenés:Current Protocols in Cytometry / Ed. J. Paul Robinson. - p. 1.12.1-1.12.16.
További szerzők:Damjanovich Sándor (1936-2017) (biofizikus) Nagy Péter (1971-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Mátyus László (1956-) (biofizikus)
Pályázati támogatás:F049025
OTKA
T043509
OTKA
T043061
OTKA
K626h8
OTKA
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM046092
Első szerző:Vámosi György (biofizikus)
Cím:IL-2 and IL-15 receptor [alfa]-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells / Vamosi G., Bodnar A., Vereb G., Jenei A., Goldman C. K., Langowski J., Toth K., Matyus L., Szollosi J., Waldmann T. A., Damjanovich S.
Dátum:2004
ISSN:0027-8424
Megjegyzések:The private alpha-chains of IL-2 and IL-15 receptors (IL-2R and IL-15R) share the signaling beta- and gamma(c)-subunits, resulting in both common and contrasting roles of IL-2 and IL-15 in T cell function. Knowledge of the cytokine-dependent subunit assembly is indispensable for understanding the paradox of distinct signaling capacities. By using fluorescence resonance energy transfer and confocal microscopy, we have shown that IL-2R alpha, IL-15R alpha, IL-2/15R beta and gamma(c)-subunits, as well as MHC class I and II glycoproteins formed supramolecular receptor clusters in lipid rafts of the T lymphoma line Kit 225 FT7.10. Fluorescence crosscorrelation microscopy demonstrated the comobility of IL-15R alpha with IL-2R alpha and MHC class I. A model was generated for subunit switching between IL-2R alpha and IL-15R alpha upon the binding of the appropriate cytokine resulting in the formation of high-affinity heterotrimeric receptors. This model suggests a direct role for the alpha-subunits, to which no definite function has been assigned so far, in tuning cellular responses to IL-2 or IL-15. In addition, both alpha-chains were at least partially homodimerized/oligomerized, which could be the basis of distinct signaling pathways by the two cytokines.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Proceedings Of The National Academy Of Sciences Of The United States Of America. - 101 : 30 (2004), p. 11082-11087. -
További szerzők:Dóczy-Bodnár Andrea (1970-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Jenei Attila (1966-) (biofizikus) Goldman, Caroline K. Langowski, Jörg Tóth Katalin (biofizikus) Mátyus László (1956-) (biofizikus) Szöllősi János (1953-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM033278
035-os BibID:PMID:8588941 WOS:A1995TF18900001
Első szerző:Vereb György (biofizikus, orvos)
Cím:Plasma-membrane-bound macromolecules are dynamically aggregated to form non-random codistribution patterns of selected functional elements. Do pattern recognition processes govern antigen presentation and intercellular interactions? / György Vereb, László Matyus, László Bene, György Panyi, Zsolt Bacsó, Margit Balázs, János Matkó, János Szöllősi, Rezső Gáspár, Sándor Damjanovich, Robert E. Dale, Carlo Pieri, Marcel Ameloot
Dátum:1995
Megjegyzések:Molecular recognition processes between cell surface elements are discussed with special reference to cell surface pattern formation of membrane-bound integral proteins. The existence, as detected by flow cytometric resonance energy transfer (Appendix), and significance of cell surface patterns involving the interleukin-2 receptor, the T-cell receptor-CD3 system, the intercellular adhesion molecule ICAM-1, and the major histocompatibility complex class I and class II molecules in the plasma membrane of lymphocytes are described. The modulation of antigen presentation by transmembrane potential changes is discussed, and a general role of transmembrane potential changes, and therefore of ion channel activities, adduced as one of the major regulatory mechanisms of cell-cell communication. A general role in the mediation and regulation of intercellular interactions is suggested for cell-surface macromolecular patterns. The dynamic pattern of protein and lipid molecules in the plasma membrane is generated by the genetic code, but has a remarkable flexibility and may be one of the major instruments of accommodation and recognition processes at the cellular level.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
cell surface
molecular pattern
energy transfer
fluorescence
flow cytometry
transmembrane potential
MHC
antigen presentation
intercellular communication
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Molecular Recognition. - 8 : 4 (1995), p. 237-246. -
További szerzők:Mátyus László (1956-) (biofizikus) Bene László (1963-) (biofizikus) Panyi György (1966-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Dale, Robert E. Pieri, Carlo Ameloot, Marcel
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

7.

001-es BibID:BIBFORM006057
Első szerző:Vereb György (biofizikus, orvos)
Cím:Effect of cyclosporin A on the membrane potential and Ca2+ level of human lymphoid cell lines and mouse thymocytes / György Vereb Jr., György Panyi, Margit Balázs, László Mátyus, János Matkó, Sándor Damjanovich
Dátum:1990
ISSN:0005-2728
Megjegyzések:The effect of the immunosuppressive cyclosporin A (CsA) on the cytosolic free Ca2+ concentration ([Ca2+]i) and membrane potential of human B and T lymphoblastoid cells and mouse thymocytes was studied in order to reveal some features of the early stage of drug-cell interaction. Cytosolic free Ca2+ concentration of the cells was measured by spectrofluorimetry using indo-1 and quin2 fluorescent calcium indicators. Membrane potential was monitored in a flow cytometer with oxonol dye. CsA applied at 2-20 micrograms/ml final concentrations caused a dose-dependent, rapid, transient rise of [Ca2+]i in all cell types. This effect could be blocked by chelating the extracellular Ca2+ with EGTA but was not sensitive to Ca2+ channel blockers verapamil and nifedipine or K+ channel blocker 4-aminopyridine. A possible explanation for the calcium mobilizing effect of CsA is an ionophore-like mode of action at the cell membrane level. Besides directly interfering with mitogenic signals, the elevation of [Ca2+]i could be responsible for an initial hyperpolarization observed in CsA-treated T lymphocytes. This hyperpolarization, however, was not detectable in B lymphoblastoid cells. A further difference between B and T cells was the diverse pattern of depolarization following CsA treatment. This variance in the behaviour of T and B lymphocytes and the diversity of membrane transport systems in its background could account for the different final outcome of the drug-cell interaction.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Cyclosporin A
Indo-1
Quin2
Membrane potential
Human lymphoid cell lines
Mouse thymocytes
Megjelenés:Biochimica et Biophysica Acta (BBA). Bioenergetics. - 1019 : 2 (1990), p. 159-165. -
További szerzők:Panyi György (1966-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változa
Borító:

8.

001-es BibID:BIBFORM006077
Első szerző:Vereb György (biofizikus, orvos)
Cím:Depletion of intracellular calcium stores facilitates the influx of extracellular calcium in platelet derived growth factor stimulated A172 glioblastoma cells / Vereb, G., Jr., Szollosi, J., Matyus, L., Balazs, M., Hyun, W. C., Feuerstein, B. G.
Dátum:1996
Megjegyzések:Calcium signaling in non-excitable cells is the consequence of calcium release from intracellular stores, at times followed by entry of extracellular calcium through the plasma membrane. To study whether entry of calcium depends upon the level of saturation of intracellular stores, we measured calcium channel opening in the plasma membrane of single confluent A172 glioblastoma cells stimulated with platelet derived growth factor (PDGF) and/or bradykinin (BK). We monitored the entry of extracellular calcium by measuring manganese quenching of Indo-1 fluorescence. PDGF raised intracellular calcium concentration ([Ca2+]i) after a dose-dependent delay (tdel) and then opened calcium channels after a dose-independent delay (tch). At higher doses (> 3 nM), BK increased [Ca2+]i after a tdel approximately 0 s, and tch decreased inversely with both dose and peak [Ca2+]i. Experiments with thapsigargin (TG), BK, and PDGF indicated that BK and PDGF share intracellular Ca2+ pools that are sensitive to TG. When these stores were depleted by treatment with BK and intracellular BAPTA, tdel did not change, but tch fell to almost 0 s in PDGF stimulated cells, indicating that depletion of calcium stores affects calcium channel opening in the plasma membrane. Our data support the capacitative model for calcium channel opening and the steady-state model describing quantal Ca2+ release from intracellular stores.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
antagonists and inhibitors
Biophysics
Bradykinin
Ca(2+)-Transporting ATPase
Calcium
Calcium Channel Blockers
Calcium Channels
Calcium Signaling
Dose-Response Relationship,Drug
drug effects
Fluorescence
Glioblastoma
Human
Hungary
Lanthanum
Manganese
metabolism
Neuroglia
pharmacology
Platelet-Derived Growth Factor
Signal Transduction
Support, Non-U.S. Gov't
Support, U.S.Gov't, Non-P.H.S.
Support, U.S.Gov't, P.H.S.
Terpenes
Thapsigargin
Tumor Cells,Cultured
Megjelenés:Cytometry. - 24 : 1 (1996), p. 64-73. -
További szerzők:Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Hyun, William C. Feuerstein, Burt G.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változa
Borító:

9.

001-es BibID:BIBFORM004743
035-os BibID:(scopus)0038492661 (wos)000184222500005
Első szerző:Vereb György (biofizikus, orvos)
Cím:Dynamic, yet structured : the cell membrane three decades after the Singer-Nicolson model / Vereb, G., Szollosi, J., Matko, J., Nagy, P., Farkas, T., Vigh, L., Matyus, L., Waldmann, T. A., Damjanovich, S.
Dátum:2003
ISSN:027-8424 (Print)
Megjegyzések:The fluid mosaic membrane model proved to be a very useful hypothesis in explaining many, but certainly not all, phenomena taking place in biological membranes. New experimental data show that the compartmentalization of membrane components can be as important for effective signal transduction as is the fluidity of the membrane. In this work, we pay tribute to the Singer-Nicolson model, which is near its 30th anniversary, honoring its basic features, "mosaicism" and "diffusion," which predict the interspersion of proteins and lipids and their ability to undergo dynamic rearrangement via Brownian motion. At the same time, modifications based on quantitative data are proposed, highlighting the often genetically predestined, yet flexible, multilevel structure implementing a vast complexity of cellular functions. This new "dynamically structured mosaic model" bears the following characteristics: emphasis is shifted from fluidity to mosaicism, which, in our interpretation, means nonrandom codistribution patterns of specific kinds of membrane proteins forming small-scale clusters at the molecular level and large-scale clusters (groups of clusters, islands) at the submicrometer level. The cohesive forces, which maintain these assemblies as principal elements of the membranes, originate from within a microdomain structure, where lipid-lipid, protein-protein, and protein-lipid interactions, as well as sub- and supramembrane (cytoskeletal, extracellular matrix, other cell) effectors, many of them genetically predestined, play equally important roles. The concept of fluidity in the original model now is interpreted as permissiveness of the architecture to continuous, dynamic restructuring of the molecular- and higher-level clusters according to the needs of the cell and as evoked by the environment.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Animals
Biophysics
Cell Membrane
chemistry
Chemistry,Physical
Diffusion
Extracellular Matrix
Fluorescence Resonance Energy Transfer
Hungary
Lipid Bilayers
Membrane Fluidity
Membrane Lipids
Membrane Microdomains
Membrane Proteins
Microscopy,Electron
Models,Biological
Motion
physiology
Proteins
Research
Signal Transduction
Support
Megjelenés:Proceedings of the National Academy of Sciences of the United States of America. - 100 : 14 (2003), p. 8053-8058. -
További szerzők:Szöllősi János (1953-) (biofizikus) Matkó János (1952-) (biológus) Nagy Péter (1971-) (biofizikus) Farkas Tamás (1971-) (biológus) Vígh L. Mátyus László (1956-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:DOI
elektronikus változat
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