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001-es BibID:BIBFORM004943
Első szerző:Gadella, Theodorus W. Jr.
Cím:Microspectroscopic imaging of nodulation factor-binding sites on living Vicia sativa roots using a novel bioactive fluorescent nodulation factor / Gadella, T. W. Jr., Vereb, G. Jr., Hadri, A. E., Rohrig, H., Schmidt, J., John, M., Schell, J., Bisseling, T.
Dátum:1997
Megjegyzések:A novel bioactive fluorescent nodulation (Nod) factor, NodRlv-IV(BODIPY FL-C16), has been synthesized by attaching a BODIPY FL-C16 acyl chain to the primary amino group of chitotetraose deacetylated at the nonreducing terminus by recombinant NodB. The binding of the fluorescent Nod factor to root systems of Vicia sativa was investigated with fluorescence spectral imaging microscopy (FSPIM) and fluorescence ratio imaging microscopy (FRIM). Spatially resolved fluorescence spectra of living and labeled Vicia sativa root systems were measured by FSPIM. Strong autofluorescence, inherent to many plant systems when excited at 488 nm, was corrected for by utilizing the difference in fluorescence emission spectra of the autofluorescence and NodRlv- IV(BODIPY FL-C16). A methodology is presented to break down the in situ fluorescence emission spectra into spatially resolved autofluorescence and BODIPY FL fluorescence spectra. Furthermore, an FRIM method was developed for correcting autofluorescence in fluorescence micrographs for this system. After autofluorescence correction it was shown that NodRlv-IV(BODIPY FL-C16) was concentrated in the root hairs, but was also bound to other parts of the root surface.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Binding Sites
chemistry
Fluorescence
methods
Microscopy
Non-U.S.Gov't
Plant Proteins
Spectrometry
Support
Megjelenés:Biophysical Journal 72 : 5 (1997), p. 1986-1996. -
További szerzők:Vereb György (1965-) (biofizikus, orvos) Hadri, Azz-Eddine Röhrig, Horst Schmidt, Jürgen John, Michael Schell, Jeff Bisseling, Ton
Internet cím:elektronikus változat
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