Találati lista | Tudóstér

001-es BibID:	BIBFORM119518
Első szerző:	Nagy Lőrinc (biológus, gyógyszer-biotechnológus)
Cím:	Universal CAR T cells targeted to HER2 with a biotin-trastuzumab soluble linker penetrate spheroids and large tumor xenografts that are inherently resistant to trastuzumab mediated ADCC / Nagy Lőrinc, Mezősi-Csaplár Marianna, Rebenku István, Vereb György, Szöőr Árpád
Dátum:	2024
ISSN:	1664-3224
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk UniCAR HER2 cell therapy
Megjelenés:	Frontiers in Immunology. - 15 (2024), p. 1-38. -
További szerzők:	Mezősi-Csaplár Marianna (1991-) Rebenku István Vereb György (1965-) (biofizikus, orvos) Szöőr Árpád (1984-) (orvos)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM109070
035-os BibID:	(cikkazonosító)2934 (Scopus)85148394136 (WoS)000955795600021
Első szerző:	Rebenku István
Cím:	Pixel-by-pixel autofluorescence corrected FRET in fluorescence microscopy improves accuracy for samples with spatially varied autofluorescence to signal ratio / Rebenku István, Lloyd Cameron B., Szöllősi János, Vereb György
Dátum:	2023
ISSN:	2045-2322
Megjegyzések:	The actual interaction between signaling species in cellular processes is often more important than their expression levels. Förster resonance energy transfer (FRET) is a popular tool for studying molecular interactions, since it is highly sensitive to proximity in the range of 2-10 nm. Spectral spillover-corrected quantitative (3-cube) FRET is a cost efective and versatile approach, which can be applied in fow cytometry and various modalities of fuorescence microscopy, but may be hampered by varying levels of autofuorescence. Here, we have implemented pixel-by-pixel autofuorescence correction in microscopy FRET measurements, exploiting cell-free calibration standards void of autofuorescence that allow the correct determination of all spectral spillover factors. We also present an ImageJ/Fiji plugin for interactive analysis of single images as well as automatic creation of quantitative FRET efciency maps from large image sets. For validation, we used bead and cell based FRET models covering a range of signal to autofuorescence ratios and FRET efciencies and compared the approach with conventional average autofuorescence/background correction. Pixel-by-pixel autofuorescence correction proved to be superior in the accuracy of results, particularly for samples with spatially varying autofuorescence and low fuorescence to autofuorescence ratios, the latter often being the case for physiological expression levels.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 13 : 1 (2023), p. 1-15. -
További szerzők:	Lloyd, Cameron B. Szöllősi János (1953-) (biofizikus) Vereb György (1965-) (biofizikus, orvos)
Pályázati támogatás:	GINOP-2.2.1-15-2017-00072 GINOP K135938 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM103569
035-os BibID:	(WoS)000851426500001 (Scopus)85146322280
Első szerző:	Rebenku István
Cím:	Taking molecular pathology to the next level : whole slide multicolor confocal imaging with the Pannoramic Confocal digital pathology scanner / István Rebenku, Ferenc A. Bartha, Tamás Katona, Barbara Zsebik, Géza Antalffy, Lili Takács, Béla Molnár, György Vereb
Dátum:	2023
ISSN:	1552-4922 1552-4930
Megjegyzések:	Abstract The emergence and fast advance of digital pathology allows the acquisition, digital storage, interactive recall and analysis of morphology at the tissue level. When applying immunohistochemistry, it also affords the correlation of morphology with the expression of one or two specific molecule of interest. The rise of fluorescence pathology scanners expands the number of detected molecules based on multiplex labelling. The Pannoramic Confocal (created by 3DHistech, Hungary) is a first-of-the-kind digital pathology scanner that affords not only multiplexed fluorescent detection on top of conventional transmission imaging, but also confocality. We have benchmarked this scanner in terms of stability, precision, light efficiency, linearity and sensitivity. X-Y stability and relocalisation precision were well below resolution limit (?50 nm). Light throughput in confocal mode was 4-5 times higher than that of a point scanning confocal microscope, yielding similar calculated confocal intensities but with the potential for improving signal to noise ratio or scan speed. Response was linear with R2 ?0.9996. Calibrated measurements showed that using indirect labeling ? 2000 molecules per cell could be well detected and imaged on the cell surface. Both standard-based and statistical post-acquisition flatfield corrections are implemented. We have also measured the point spread function (PSF) of the instrument. The dimensions of the PSF are somewhat larger and less symmetric than of the theoretical PSF of a conventional CLSM, however, the spatial homogeneity of these parameters allows for obtaining a specific system PSF for each optical path and using it for optional on-the-fly deconvolution. In conclusion, the Pannoramic Confocal provides sensitive, quantitative widefield and confocal detection of multiplexed fluorescence signals, with optical sectioning and 3D reconstruction, in addition to brightfield transmission imaging. High speed scanning of large samples, analysis of tissue heterogeneity, and detection of rare events open up new ways for quantitatively analyzing tissue sections, organoid cultures or large numbers of adherent cells.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk 3-dimensional virtual slide deconvolution digital pathology multispectral fluorescence imaging widefield aperture correlated confocal imaging
Megjelenés:	Cytometry Part A. - 103 : 3 (2023), p. 198-207. -
További szerzők:	Bartha Ferenc Katona Tamás (1991-) (informatikus) Zsebik Barbara (1977-) (biofizikus) Antalffy Géza Takács Lili (1969-) (szemész) Molnár Béla Vereb György (1965-) (biofizikus, orvos)
Pályázati támogatás:	GINOP-2.2.1-15-2017-00072 GINOP OTKA K135938 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: