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Dátum csökkenő
1.
001-es BibID:
BIBFORM006036
Első szerző:
Gáspár Rezső (biofizikus)
Cím:
Effects of bretylium tosylate on voltage-gated potassium channels in human T lymphocytes / Gaspar, R., Panyi, G., Ypey, D. L., Krasznai, Z., Vereb, G., Pieri, C., Damjanovich, S.
Dátum:
1994
Megjegyzések:
Using the patch-clamp technique, we determined that bretylium tosylate, a quaternary ammonium compound possessing immunomodulating activity, decreased the whole-cell K+ current in human T lymphocytes, in a dose-dependent manner, in the 0.05-5 mM extracellular concentration range. Bretylium tosylate prolonged the recovery from inactivation and accelerated the inactivation and deactivation of the K+ current but did not influence the kinetics of activation or the voltage dependence of activation and steady state inactivation of the K+ conductance. The percentage of drug-induced block was independent of membrane potential. K+ channel block by bretylium tosylate was partially and slowly removable by washing with drug-free extracellular solution. Bovine serum albumin (10 mg/ml) in the bath lifted the drug-induced block almost instantaneously, although not completely. In control experiments bovine serum albumin increased the inactivation time constant of the K+ channels but left the peak K+ current amplitude unaffected. On the basis of the experimental evidence, a gating-dependent allosteric interaction is suggested for the mechanism of drug action. The effective dose range, time of exposure, and reversibility of bretylium tosylate-induced K+ channel block correlated well with the same parameters of the drug-induced inhibition of T lymphocyte activation. The reported effects of bretylium tosylate on T cell mitogenesis can be regarded partly as a consequence of its blocking effects on voltage-gated K+ channels.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Bretylium Tosylate
Cell Membrane
drug effects
Electrophysiology
Human
Hungary
In Vitro
Ion Channel Gating
Kinetics
Lymphocytes
pharmacology
physiology
Potassium
Potassium Channels
Support,Non-U.S.Gov't
T-Lymphocytes
Megjelenés:
Molecular pharmacology. - 46 : 4 (1994), p. 762-766. -
További szerzők:
Panyi György (1966-) (biofizikus)
Ypey, Dirk L.
Krasznai Zoltán (1950-) (biofizikus)
Vereb György (1965-) (biofizikus, orvos)
Pieri, Carlo
Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:
elektronikus változat
Borító:
Saját polcon:
2.
001-es BibID:
BIBFORM006044
Első szerző:
Matkó János (biológus)
Cím:
Biphasic effect of extracellular ATP on the membrane potential of mouse thymocytes / Matko J., Nagy P., Panyi G., Vereb G. Jr., Bene L., Matyus L., Damjanovich S.
Dátum:
1993
Megjegyzések:
Extracellular ATP induced changes in the membrane potential of thymocytes from BALB/c mice were analyzed. At concentrations below 0.1 mM, ATP hyperpolarizes the cell membrane on the time scale of development of the Ca(2+)-signal. After a longer time hyperpolarization turns to depolarization. ATP concentrations higher than 0.5 mM caused rapid depolarization without previous hyperpolarization. Verapamil, quinine or the absence of extracellular Ca2+ blocked the hyperpolarization by ATP. In Na(+)-free medium the magnitude of depolarization decreased. Our data suggest a contribution of Ca(2+)-activated K+ channels to the hyperpolarizing effect of ATP at lower concentrations. The direction of membrane potential changes is determined presumably by a sensitive balance of ATP-receptor mediated Ca(2+)- and Na(+)-influx and the Ca(2+)-activated K(+)-channel activity.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Adenosine Triphosphate
Animal
Biophysics
Cell Membrane
cytology
drug effects
Hungary
Kinetics
Membrane Potentials
Mice
Mice,Inbred BALB C
pharmacology
physiology
Quinine
Support,Non-U.S.Gov't
Thymus Gland
Verapamil
Megjelenés:
Biochemical and Biophysical Research Communications. - 191 : 2 (1993), p. 378-384. -
További szerzők:
Nagy Péter (1971-) (biofizikus)
Panyi György (1966-) (biofizikus)
Vereb György (1965-) (biofizikus, orvos)
Bene László (1963-) (biofizikus)
Mátyus László (1956-) (biofizikus)
Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:
elektronikus változat
DOI
Intézményi repozitóriumban (DEA) tárolt változa
Borító:
Saját polcon:
3.
001-es BibID:
BIBFORM033278
035-os BibID:
PMID:8588941 WOS:A1995TF18900001
Első szerző:
Vereb György (biofizikus, orvos)
Cím:
Plasma-membrane-bound macromolecules are dynamically aggregated to form non-random codistribution patterns of selected functional elements. Do pattern recognition processes govern antigen presentation and intercellular interactions? / György Vereb, László Matyus, László Bene, György Panyi, Zsolt Bacsó, Margit Balázs, János Matkó, János Szöllősi, Rezső Gáspár, Sándor Damjanovich, Robert E. Dale, Carlo Pieri, Marcel Ameloot
Dátum:
1995
Megjegyzések:
Molecular recognition processes between cell surface elements are discussed with special reference to cell surface pattern formation of membrane-bound integral proteins. The existence, as detected by flow cytometric resonance energy transfer (Appendix), and significance of cell surface patterns involving the interleukin-2 receptor, the T-cell receptor-CD3 system, the intercellular adhesion molecule ICAM-1, and the major histocompatibility complex class I and class II molecules in the plasma membrane of lymphocytes are described. The modulation of antigen presentation by transmembrane potential changes is discussed, and a general role of transmembrane potential changes, and therefore of ion channel activities, adduced as one of the major regulatory mechanisms of cell-cell communication. A general role in the mediation and regulation of intercellular interactions is suggested for cell-surface macromolecular patterns. The dynamic pattern of protein and lipid molecules in the plasma membrane is generated by the genetic code, but has a remarkable flexibility and may be one of the major instruments of accommodation and recognition processes at the cellular level.
Tárgyszavak:
Orvostudományok
Egészségtudományok
idegen nyelvű folyóiratközlemény külföldi lapban
cell surface
molecular pattern
energy transfer
fluorescence
flow cytometry
transmembrane potential
MHC
antigen presentation
intercellular communication
egyetemen (Magyarországon) készült közlemény
Megjelenés:
Journal of Molecular Recognition. - 8 : 4 (1995), p. 237-246. -
További szerzők:
Mátyus László (1956-) (biofizikus)
Bene László (1963-) (biofizikus)
Panyi György (1966-) (biofizikus)
Bacsó Zsolt (1963-) (biofizikus)
Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus)
Matkó János (1952-) (biológus)
Szöllősi János (1953-) (biofizikus)
Gáspár Rezső (1944-) (biofizikus)
Damjanovich Sándor (1936-2017) (biofizikus)
Dale, Robert E.
Pieri, Carlo
Ameloot, Marcel
Internet cím:
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:
4.
001-es BibID:
BIBFORM006057
Első szerző:
Vereb György (biofizikus, orvos)
Cím:
Effect of cyclosporin A on the membrane potential and Ca2+ level of human lymphoid cell lines and mouse thymocytes / György Vereb Jr., György Panyi, Margit Balázs, László Mátyus, János Matkó, Sándor Damjanovich
Dátum:
1990
ISSN:
0005-2728
Megjegyzések:
The effect of the immunosuppressive cyclosporin A (CsA) on the cytosolic free Ca2+ concentration ([Ca2+]i) and membrane potential of human B and T lymphoblastoid cells and mouse thymocytes was studied in order to reveal some features of the early stage of drug-cell interaction. Cytosolic free Ca2+ concentration of the cells was measured by spectrofluorimetry using indo-1 and quin2 fluorescent calcium indicators. Membrane potential was monitored in a flow cytometer with oxonol dye. CsA applied at 2-20 micrograms/ml final concentrations caused a dose-dependent, rapid, transient rise of [Ca2+]i in all cell types. This effect could be blocked by chelating the extracellular Ca2+ with EGTA but was not sensitive to Ca2+ channel blockers verapamil and nifedipine or K+ channel blocker 4-aminopyridine. A possible explanation for the calcium mobilizing effect of CsA is an ionophore-like mode of action at the cell membrane level. Besides directly interfering with mitogenic signals, the elevation of [Ca2+]i could be responsible for an initial hyperpolarization observed in CsA-treated T lymphocytes. This hyperpolarization, however, was not detectable in B lymphoblastoid cells. A further difference between B and T cells was the diverse pattern of depolarization following CsA treatment. This variance in the behaviour of T and B lymphocytes and the diversity of membrane transport systems in its background could account for the different final outcome of the drug-cell interaction.
Tárgyszavak:
Orvostudományok
Elméleti orvostudományok
idegen nyelvű folyóiratközlemény külföldi lapban
Cyclosporin A
Indo-1
Quin2
Membrane potential
Human lymphoid cell lines
Mouse thymocytes
Megjelenés:
Biochimica et Biophysica Acta (BBA). Bioenergetics. - 1019 : 2 (1990), p. 159-165. -
További szerzők:
Panyi György (1966-) (biofizikus)
Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus)
Mátyus László (1956-) (biofizikus)
Matkó János (1952-) (biológus)
Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:
Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változa
Borító:
Saját polcon:
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